MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma

Abstract Background Neuroblastoma (NB) represents the most common extracranial solid tumor in children. Accumulating evidence shows that microRNAs (miRs) play an important role in the carcinogenesis of NB. Here, we investigated the biological function of miR-1247 in NB in vitro. Methods/results We...

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Autores principales:	Wu,Tingting, Lin,Yun, Xie,Zhongguo
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	Neuroblastoma miR-1247 Proliferation Apoptosis ZNF346
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100212
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spelling	oai:scielo:S0716-976020180001002122018-06-25MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastomaWu,TingtingLin,YunXie,Zhongguo Neuroblastoma miR-1247 Proliferation Apoptosis ZNF346 Abstract Background Neuroblastoma (NB) represents the most common extracranial solid tumor in children. Accumulating evidence shows that microRNAs (miRs) play an important role in the carcinogenesis of NB. Here, we investigated the biological function of miR-1247 in NB in vitro. Methods/results We found miR-1247 was downregulated in NB tissues and cells using quantitative PCR analysis. Gain- and loss-of-function studies demonstrated that miR-1247 significantly suppressed cell proliferation and induced cell cycle G0/G1 phase arrest and cell apoptosis of NB cells in vitro by using MTT, colony formation assay and Flow cytometry analysis. Luciferase assay suggested ZNF346 was the target of miR-1247 and its expression could be down-regulated by miR-1247 overexpression using Western blotting. Furthermore, downregulation of ZNF346 by siRNA performed similar effects with overexpression of miR-1247 in NB cells. Conclusions Our findings suggested miR-1247 directly targeted to repress ZNF346 expression, thus suppressing the progression of NB, which might be a novel therapeutic target against NB.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100212en10.1186/s40659-018-0162-y
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Neuroblastoma miR-1247 Proliferation Apoptosis ZNF346
spellingShingle	Neuroblastoma miR-1247 Proliferation Apoptosis ZNF346 Wu,Tingting Lin,Yun Xie,Zhongguo MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma
description	Abstract Background Neuroblastoma (NB) represents the most common extracranial solid tumor in children. Accumulating evidence shows that microRNAs (miRs) play an important role in the carcinogenesis of NB. Here, we investigated the biological function of miR-1247 in NB in vitro. Methods/results We found miR-1247 was downregulated in NB tissues and cells using quantitative PCR analysis. Gain- and loss-of-function studies demonstrated that miR-1247 significantly suppressed cell proliferation and induced cell cycle G0/G1 phase arrest and cell apoptosis of NB cells in vitro by using MTT, colony formation assay and Flow cytometry analysis. Luciferase assay suggested ZNF346 was the target of miR-1247 and its expression could be down-regulated by miR-1247 overexpression using Western blotting. Furthermore, downregulation of ZNF346 by siRNA performed similar effects with overexpression of miR-1247 in NB cells. Conclusions Our findings suggested miR-1247 directly targeted to repress ZNF346 expression, thus suppressing the progression of NB, which might be a novel therapeutic target against NB.
author	Wu,Tingting Lin,Yun Xie,Zhongguo
author_facet	Wu,Tingting Lin,Yun Xie,Zhongguo
author_sort	Wu,Tingting
title	MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma
title_short	MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma
title_full	MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma
title_fullStr	MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma
title_full_unstemmed	MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma
title_sort	microrna-1247 inhibits cell proliferation by directly targeting znf346 in childhood neuroblastoma
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100212
work_keys_str_mv	AT wutingting microrna1247inhibitscellproliferationbydirectlytargetingznf346inchildhoodneuroblastoma AT linyun microrna1247inhibitscellproliferationbydirectlytargetingznf346inchildhoodneuroblastoma AT xiezhongguo microrna1247inhibitscellproliferationbydirectlytargetingznf346inchildhoodneuroblastoma
_version_	1718441570668118016

MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma

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