Identification of key genes and pathways involved in response to pain in goat and sheep by transcriptome sequencing

Abstract Purpose: This aim of this study was to investigate the key genes and pathways involved in the response to pain in goat and sheep by transcriptome sequencing. Methods: Chronic pain was induced with the injection of the complete Freund's adjuvant (CFA) in sheep and goats. The animals w...

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Autores principales:	Deng,Xiuling, Wang,Dong, Wang,Shenyuan, Wang,Haisheng, Zhou,Huanmin
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	Chronic pain Transcriptome sequencing Differentially expressed genes Gene ontology Goat Sheep
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100221
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spelling	oai:scielo:S0716-976020180001002212018-09-05Identification of key genes and pathways involved in response to pain in goat and sheep by transcriptome sequencingDeng,XiulingWang,DongWang,ShenyuanWang,HaishengZhou,Huanmin Chronic pain Transcriptome sequencing Differentially expressed genes Gene ontology Goat Sheep Abstract Purpose: This aim of this study was to investigate the key genes and pathways involved in the response to pain in goat and sheep by transcriptome sequencing. Methods: Chronic pain was induced with the injection of the complete Freund's adjuvant (CFA) in sheep and goats. The animals were divided into four groups: CFA-treated sheep, control sheep, CFA-treated goat, and control goat groups (n = 3 in each group). The dorsal root ganglions of these animals were isolated and used for the construction of a cDNA library and transcriptome sequencing. Differentially expressed genes (DEGs) were identified in CFA-induced sheep and goats and gene ontology (GO) enrichment analysis was performed. Results: In total, 1748 and 2441 DEGs were identified in CFA-treated goat and sheep, respectively. The DEGs identified in CFA-treated goats, such as C-C motif chemokine ligand 27 (CCL27), glutamate receptor 2 (GRIA2), and sodium voltage-gated channel alpha subunit 3 (SCN3A), were mainly enriched in GO functions associated with N-methyl-D-aspartate (NMDA) receptor, inflammatory response, and immune response. The DEGs identified in CFA-treated sheep, such as gamma-aminobutyric acid (GABA)-related DEGs (gamma-aminobutyric acid type A receptor gamma 3 subunit [GABRG3], GABRB2, and GABRB1), SCN9A, and transient receptor potential cation channel subfamily V member 1 (TRPV1), were mainly enriched in GO functions related to neuroactive ligand-receptor interaction, NMDA receptor, and defense response. Conclusions: Our data indicate that NMDA receptor, inflammatory response, and immune response as well as key DEGs such as CCL27, GRIA2, and SCN3A may regulate the process of pain response during chronic pain in goats. Neuroactive ligand-receptor interaction and NMDA receptor as well as GABA-related DEGs, SCN9A, and TRPV1 may modulate the process of response to pain in sheep. These DEGs may serve as drug targets for preventing chronic pain.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100221en10.1186/s40659-018-0174-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Chronic pain Transcriptome sequencing Differentially expressed genes Gene ontology Goat Sheep
spellingShingle	Chronic pain Transcriptome sequencing Differentially expressed genes Gene ontology Goat Sheep Deng,Xiuling Wang,Dong Wang,Shenyuan Wang,Haisheng Zhou,Huanmin Identification of key genes and pathways involved in response to pain in goat and sheep by transcriptome sequencing
description	Abstract Purpose: This aim of this study was to investigate the key genes and pathways involved in the response to pain in goat and sheep by transcriptome sequencing. Methods: Chronic pain was induced with the injection of the complete Freund's adjuvant (CFA) in sheep and goats. The animals were divided into four groups: CFA-treated sheep, control sheep, CFA-treated goat, and control goat groups (n = 3 in each group). The dorsal root ganglions of these animals were isolated and used for the construction of a cDNA library and transcriptome sequencing. Differentially expressed genes (DEGs) were identified in CFA-induced sheep and goats and gene ontology (GO) enrichment analysis was performed. Results: In total, 1748 and 2441 DEGs were identified in CFA-treated goat and sheep, respectively. The DEGs identified in CFA-treated goats, such as C-C motif chemokine ligand 27 (CCL27), glutamate receptor 2 (GRIA2), and sodium voltage-gated channel alpha subunit 3 (SCN3A), were mainly enriched in GO functions associated with N-methyl-D-aspartate (NMDA) receptor, inflammatory response, and immune response. The DEGs identified in CFA-treated sheep, such as gamma-aminobutyric acid (GABA)-related DEGs (gamma-aminobutyric acid type A receptor gamma 3 subunit [GABRG3], GABRB2, and GABRB1), SCN9A, and transient receptor potential cation channel subfamily V member 1 (TRPV1), were mainly enriched in GO functions related to neuroactive ligand-receptor interaction, NMDA receptor, and defense response. Conclusions: Our data indicate that NMDA receptor, inflammatory response, and immune response as well as key DEGs such as CCL27, GRIA2, and SCN3A may regulate the process of pain response during chronic pain in goats. Neuroactive ligand-receptor interaction and NMDA receptor as well as GABA-related DEGs, SCN9A, and TRPV1 may modulate the process of response to pain in sheep. These DEGs may serve as drug targets for preventing chronic pain.
author	Deng,Xiuling Wang,Dong Wang,Shenyuan Wang,Haisheng Zhou,Huanmin
author_facet	Deng,Xiuling Wang,Dong Wang,Shenyuan Wang,Haisheng Zhou,Huanmin
author_sort	Deng,Xiuling
title	Identification of key genes and pathways involved in response to pain in goat and sheep by transcriptome sequencing
title_short	Identification of key genes and pathways involved in response to pain in goat and sheep by transcriptome sequencing
title_full	Identification of key genes and pathways involved in response to pain in goat and sheep by transcriptome sequencing
title_fullStr	Identification of key genes and pathways involved in response to pain in goat and sheep by transcriptome sequencing
title_full_unstemmed	Identification of key genes and pathways involved in response to pain in goat and sheep by transcriptome sequencing
title_sort	identification of key genes and pathways involved in response to pain in goat and sheep by transcriptome sequencing
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100221
work_keys_str_mv	AT dengxiuling identificationofkeygenesandpathwaysinvolvedinresponsetopainingoatandsheepbytranscriptomesequencing AT wangdong identificationofkeygenesandpathwaysinvolvedinresponsetopainingoatandsheepbytranscriptomesequencing AT wangshenyuan identificationofkeygenesandpathwaysinvolvedinresponsetopainingoatandsheepbytranscriptomesequencing AT wanghaisheng identificationofkeygenesandpathwaysinvolvedinresponsetopainingoatandsheepbytranscriptomesequencing AT zhouhuanmin identificationofkeygenesandpathwaysinvolvedinresponsetopainingoatandsheepbytranscriptomesequencing
_version_	1718441573070405632

Identification of key genes and pathways involved in response to pain in goat and sheep by transcriptome sequencing

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