Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis

Abstract Background: The domestic cat serves as an animal model for assisted reproductive studies of endangered felid species. To date, there are no data on the protein alterations following cryopreservation of oocytes in felid family. Methods: Immature (germinal vesicle) domestic cat oocytes were...

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Autores principales:	Turathum,Bongkoch, Roytrakul,Sittiruk, Changsangfa,Chinarat, Sroyraya,Morakot, Tanasawet,Supita, Kitiyanant,Yindee, Saikhun,Kulnasan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	Proteome Cat oocyte Cryopreservation Vitrification
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100223
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spelling	oai:scielo:S0716-976020180001002232018-09-05Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysisTurathum,BongkochRoytrakul,SittirukChangsangfa,ChinaratSroyraya,MorakotTanasawet,SupitaKitiyanant,YindeeSaikhun,Kulnasan Proteome Cat oocyte Cryopreservation Vitrification Abstract Background: The domestic cat serves as an animal model for assisted reproductive studies of endangered felid species. To date, there are no data on the protein alterations following cryopreservation of oocytes in felid family. Methods: Immature (germinal vesicle) domestic cat oocytes were vitrified in the vitrification solution containing 35% ethylene glycol, 20% DMSO and 0.5 mM sucrose. The vitrified-warmed oocytes were matured (metaphase II) in vitro and subjected to proteomic analysis using 1DE SDS-PAGE prefractionation combined with LC-MS/MS. Results: A total of 1712 proteins were identified in in vitro matured oocytes. Of the 1712 proteins, 1454 proteins were found in both groups, whereas, 258 proteins were differentially expressed between control and vitrified-warmed groups. In vitrified-warmed oocytes, the missing proteins were membrane and nuclear proteins; whereas, apoptosis and DNA repair proteins were overrepresented. Conclusions: The identified missing and overexpressed proteins in vitrified-warmed oocytes represent potential markers of cryoinjuries and the developmental pathways of oocytes. The findings of differential expressed proteins may contribute to effective ways of proteome analysis of oocyte/embryo quality in order to assess safety of cryopreservation in felid species.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100223en10.1186/s40659-018-0176-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Proteome Cat oocyte Cryopreservation Vitrification
spellingShingle	Proteome Cat oocyte Cryopreservation Vitrification Turathum,Bongkoch Roytrakul,Sittiruk Changsangfa,Chinarat Sroyraya,Morakot Tanasawet,Supita Kitiyanant,Yindee Saikhun,Kulnasan Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
description	Abstract Background: The domestic cat serves as an animal model for assisted reproductive studies of endangered felid species. To date, there are no data on the protein alterations following cryopreservation of oocytes in felid family. Methods: Immature (germinal vesicle) domestic cat oocytes were vitrified in the vitrification solution containing 35% ethylene glycol, 20% DMSO and 0.5 mM sucrose. The vitrified-warmed oocytes were matured (metaphase II) in vitro and subjected to proteomic analysis using 1DE SDS-PAGE prefractionation combined with LC-MS/MS. Results: A total of 1712 proteins were identified in in vitro matured oocytes. Of the 1712 proteins, 1454 proteins were found in both groups, whereas, 258 proteins were differentially expressed between control and vitrified-warmed groups. In vitrified-warmed oocytes, the missing proteins were membrane and nuclear proteins; whereas, apoptosis and DNA repair proteins were overrepresented. Conclusions: The identified missing and overexpressed proteins in vitrified-warmed oocytes represent potential markers of cryoinjuries and the developmental pathways of oocytes. The findings of differential expressed proteins may contribute to effective ways of proteome analysis of oocyte/embryo quality in order to assess safety of cryopreservation in felid species.
author	Turathum,Bongkoch Roytrakul,Sittiruk Changsangfa,Chinarat Sroyraya,Morakot Tanasawet,Supita Kitiyanant,Yindee Saikhun,Kulnasan
author_facet	Turathum,Bongkoch Roytrakul,Sittiruk Changsangfa,Chinarat Sroyraya,Morakot Tanasawet,Supita Kitiyanant,Yindee Saikhun,Kulnasan
author_sort	Turathum,Bongkoch
title	Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
title_short	Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
title_full	Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
title_fullStr	Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
title_full_unstemmed	Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
title_sort	missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100223
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_version_	1718441573549604864

Missing and overexpressing proteins in domestic cat oocytes following vitrification and in vitro maturation as revealed by proteomic analysis

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