Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine sponges

Abstract Background: Pectinase enzymes catalyze the breakdown of pectin, a key component of the plant cell wall. At industrial level, pectinases are used in diverse applications, especially in food-processing industry. Currently, most of the industrial pectinases have optimal activity at mesophilic...

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Autores principales:	Poveda,Gabriela, Gil-Durán,Carlos, Vaca,Inmaculada, Levicán,Gloria, Chávez,Renato
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	Cold-active pectinases Antarctic marine sponges Filamentous fungi Geomyces sp
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100224
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spelling	oai:scielo:S0716-976020180001002242018-10-18Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine spongesPoveda,GabrielaGil-Durán,CarlosVaca,InmaculadaLevicán,GloriaChávez,Renato Cold-active pectinases Antarctic marine sponges Filamentous fungi Geomyces sp Abstract Background: Pectinase enzymes catalyze the breakdown of pectin, a key component of the plant cell wall. At industrial level, pectinases are used in diverse applications, especially in food-processing industry. Currently, most of the industrial pectinases have optimal activity at mesophilic temperatures. On the contrary, very little is known about the pectinolytic activities from organisms from cold climates such as Antarctica. In this work, 27 filamentous fungi isolated from marine sponges collected in King George Island, Antarctica, were screened as new source of cold-active pectinases. Results: In semi-quantitative plate assays, 8 out 27 of these isolates showed pectinolytic activities at 15 °C and one of them, Geomyces sp. strain F09-T3-2, showed the highest production of pectinases in liquid medium containing pectin as sole carbon source. More interesting, Geomyces sp. F09-T3-2 showed optimal pectinolytic activity at 30 °C, 10 °C under the temperature of currently available commercial mesophilic pectinases. Conclusion: Filamentous fungi associated with Antarctic marine sponges are a promising source of pectinolytic activity. In particular, pectinases from Geomyces sp. F09-T3-2 may be potentially suitable for biotechnological applications needing cold-active pectinases. To the best of our knowledge, this is the first report describing the production of pectinolytic activity from filamentous fungi from any environment in Antarctica.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100224en10.1186/s40659-018-0177-4
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cold-active pectinases Antarctic marine sponges Filamentous fungi Geomyces sp
spellingShingle	Cold-active pectinases Antarctic marine sponges Filamentous fungi Geomyces sp Poveda,Gabriela Gil-Durán,Carlos Vaca,Inmaculada Levicán,Gloria Chávez,Renato Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine sponges
description	Abstract Background: Pectinase enzymes catalyze the breakdown of pectin, a key component of the plant cell wall. At industrial level, pectinases are used in diverse applications, especially in food-processing industry. Currently, most of the industrial pectinases have optimal activity at mesophilic temperatures. On the contrary, very little is known about the pectinolytic activities from organisms from cold climates such as Antarctica. In this work, 27 filamentous fungi isolated from marine sponges collected in King George Island, Antarctica, were screened as new source of cold-active pectinases. Results: In semi-quantitative plate assays, 8 out 27 of these isolates showed pectinolytic activities at 15 °C and one of them, Geomyces sp. strain F09-T3-2, showed the highest production of pectinases in liquid medium containing pectin as sole carbon source. More interesting, Geomyces sp. F09-T3-2 showed optimal pectinolytic activity at 30 °C, 10 °C under the temperature of currently available commercial mesophilic pectinases. Conclusion: Filamentous fungi associated with Antarctic marine sponges are a promising source of pectinolytic activity. In particular, pectinases from Geomyces sp. F09-T3-2 may be potentially suitable for biotechnological applications needing cold-active pectinases. To the best of our knowledge, this is the first report describing the production of pectinolytic activity from filamentous fungi from any environment in Antarctica.
author	Poveda,Gabriela Gil-Durán,Carlos Vaca,Inmaculada Levicán,Gloria Chávez,Renato
author_facet	Poveda,Gabriela Gil-Durán,Carlos Vaca,Inmaculada Levicán,Gloria Chávez,Renato
author_sort	Poveda,Gabriela
title	Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine sponges
title_short	Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine sponges
title_full	Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine sponges
title_fullStr	Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine sponges
title_full_unstemmed	Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine sponges
title_sort	cold-active pectinolytic activity produced by filamentous fungi associated with antarctic marine sponges
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100224
work_keys_str_mv	AT povedagabriela coldactivepectinolyticactivityproducedbyfilamentousfungiassociatedwithantarcticmarinesponges AT gildurancarlos coldactivepectinolyticactivityproducedbyfilamentousfungiassociatedwithantarcticmarinesponges AT vacainmaculada coldactivepectinolyticactivityproducedbyfilamentousfungiassociatedwithantarcticmarinesponges AT levicangloria coldactivepectinolyticactivityproducedbyfilamentousfungiassociatedwithantarcticmarinesponges AT chavezrenato coldactivepectinolyticactivityproducedbyfilamentousfungiassociatedwithantarcticmarinesponges
_version_	1718441573845303296

Cold-active pectinolytic activity produced by filamentous fungi associated with Antarctic marine sponges

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