Knockdown of lncRNA MIR31HG inhibits cell proliferation in human HaCaT keratinocytes
Abstract Background: Psoriasis is a complex, chronic inflammatory skin disease with substantial negative effects on patient quality of life. Long non-coding RNAs (lncRNAs) are able to be involved in multitudes of cellular processes in diverse human diseases. This study aimed to investigate the pote...
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Sociedad de Biología de Chile
2018
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oai:scielo:S0716-976020180001002262018-10-18Knockdown of lncRNA MIR31HG inhibits cell proliferation in human HaCaT keratinocytesGao,JintaoChen,FangruHua,MingchunGuo,JunfanNong,YuejuanTang,QinyanZhong,FengxiaQin,Linxiu Knockdown LncRNA MIR31HG Proliferation HaCaT keratinocytes Abstract Background: Psoriasis is a complex, chronic inflammatory skin disease with substantial negative effects on patient quality of life. Long non-coding RNAs (lncRNAs) are able to be involved in multitudes of cellular processes in diverse human diseases. This study aimed to investigate the potential involvement of lncRNA MIR31HG in HaCaT keratinocytes proliferation. Results: The study showed that MIR31HG was significantly elevated in the lesional psoriatic skin compared with normal individuals’ skin. Knockdown of MIR31HG inhibited HaCaT keratinocytes proliferation. Flow cytometry analysis showed that siRNA-mediated MIR31HG depletion induced cell cycle arrest in the G2/M phase. In addition, MIR31HG expression was found to be dependent on NF-κB activation. Conclusions: NF-κB activation mediated MIR31HG upregulation plays an important role in the regulation of HaCaT keratinocytes proliferation. It could be a potential diagnostic biomarker and therapeutic target for psoriasis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100226en10.1186/s40659-018-0181-8 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Knockdown LncRNA MIR31HG Proliferation HaCaT keratinocytes |
| spellingShingle |
Knockdown LncRNA MIR31HG Proliferation HaCaT keratinocytes Gao,Jintao Chen,Fangru Hua,Mingchun Guo,Junfan Nong,Yuejuan Tang,Qinyan Zhong,Fengxia Qin,Linxiu Knockdown of lncRNA MIR31HG inhibits cell proliferation in human HaCaT keratinocytes |
| description |
Abstract Background: Psoriasis is a complex, chronic inflammatory skin disease with substantial negative effects on patient quality of life. Long non-coding RNAs (lncRNAs) are able to be involved in multitudes of cellular processes in diverse human diseases. This study aimed to investigate the potential involvement of lncRNA MIR31HG in HaCaT keratinocytes proliferation. Results: The study showed that MIR31HG was significantly elevated in the lesional psoriatic skin compared with normal individuals’ skin. Knockdown of MIR31HG inhibited HaCaT keratinocytes proliferation. Flow cytometry analysis showed that siRNA-mediated MIR31HG depletion induced cell cycle arrest in the G2/M phase. In addition, MIR31HG expression was found to be dependent on NF-κB activation. Conclusions: NF-κB activation mediated MIR31HG upregulation plays an important role in the regulation of HaCaT keratinocytes proliferation. It could be a potential diagnostic biomarker and therapeutic target for psoriasis. |
| author |
Gao,Jintao Chen,Fangru Hua,Mingchun Guo,Junfan Nong,Yuejuan Tang,Qinyan Zhong,Fengxia Qin,Linxiu |
| author_facet |
Gao,Jintao Chen,Fangru Hua,Mingchun Guo,Junfan Nong,Yuejuan Tang,Qinyan Zhong,Fengxia Qin,Linxiu |
| author_sort |
Gao,Jintao |
| title |
Knockdown of lncRNA MIR31HG inhibits cell proliferation in human HaCaT keratinocytes |
| title_short |
Knockdown of lncRNA MIR31HG inhibits cell proliferation in human HaCaT keratinocytes |
| title_full |
Knockdown of lncRNA MIR31HG inhibits cell proliferation in human HaCaT keratinocytes |
| title_fullStr |
Knockdown of lncRNA MIR31HG inhibits cell proliferation in human HaCaT keratinocytes |
| title_full_unstemmed |
Knockdown of lncRNA MIR31HG inhibits cell proliferation in human HaCaT keratinocytes |
| title_sort |
knockdown of lncrna mir31hg inhibits cell proliferation in human hacat keratinocytes |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2018 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100226 |
| work_keys_str_mv |
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| _version_ |
1718441574438797312 |