Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis

Abstract Background: New evidence demonstrates that aging and dyslipidemia are closely associated with oxidative stress, DNA damage and apoptosis in some cells and extravascular tissues. However, in monocytes, which are naturally involved in progression and/or resolution of plaque in atherosclerosi...

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Autores principales:	Jacinto,Thais A., Meireles,Giselle S., Dias,Ananda T., Aires,Rafaela, Porto,Marcella L., Gava,Agata L., Vasquez,Elisardo C., Pereira,Thiago Melo C., Campagnaro,Bianca P., Meyrelles,Silvana S.
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	apoE knockout mice Atherosclerosis Proinflammatory cytokines Apoptosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100228
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spelling	oai:scielo:S0716-976020180001002282018-10-18Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosisJacinto,Thais A.Meireles,Giselle S.Dias,Ananda T.Aires,RafaelaPorto,Marcella L.Gava,Agata L.Vasquez,Elisardo C.Pereira,Thiago Melo C.Campagnaro,Bianca P.Meyrelles,Silvana S. apoE knockout mice Atherosclerosis Proinflammatory cytokines Apoptosis Abstract Background: New evidence demonstrates that aging and dyslipidemia are closely associated with oxidative stress, DNA damage and apoptosis in some cells and extravascular tissues. However, in monocytes, which are naturally involved in progression and/or resolution of plaque in atherosclerosis, this concurrence has not yet been fully investigated. In this study, we evaluated the influence of aging and hypercholesterolemia on serum pro-inflammatory cytokines, oxidative stress, DNA damage and apoptosis in monocytes from apolipoprotein E-deficient (apoE-/-) mice compared with age-matched wild-type C57BL/6 (WT) mice. Experiments were performed in young (2-months) and in old (18-months) male wild-type (WT) and apoE-/- mice. Results: Besides the expected differences in serum lipid profile and plaque formation, we observed that atherosclerotic mice exhibited a significant increase in monocytosis and in serum levels of pro-inflammatory cytokines compared to WT mice. Moreover, it was observed that the overproduction of ROS, led to an increased DNA fragmentation and, consequently, apoptosis in monocytes from normocholesterolemic old mice, which was aggravated in age-matched atherosclerotic mice. Conclusions: In this study, we demonstrate that a pro-inflammatory systemic status is associated with an impairment of functionality of monocytes during aging and that these parameters are fundamental extra-arterial contributors to the aggravation of atherosclerosis. The present data open new avenues for the development of future strategies with the purpose of treating atherosclerosis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100228en10.1186/s40659-018-0182-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	apoE knockout mice Atherosclerosis Proinflammatory cytokines Apoptosis
spellingShingle	apoE knockout mice Atherosclerosis Proinflammatory cytokines Apoptosis Jacinto,Thais A. Meireles,Giselle S. Dias,Ananda T. Aires,Rafaela Porto,Marcella L. Gava,Agata L. Vasquez,Elisardo C. Pereira,Thiago Melo C. Campagnaro,Bianca P. Meyrelles,Silvana S. Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis
description	Abstract Background: New evidence demonstrates that aging and dyslipidemia are closely associated with oxidative stress, DNA damage and apoptosis in some cells and extravascular tissues. However, in monocytes, which are naturally involved in progression and/or resolution of plaque in atherosclerosis, this concurrence has not yet been fully investigated. In this study, we evaluated the influence of aging and hypercholesterolemia on serum pro-inflammatory cytokines, oxidative stress, DNA damage and apoptosis in monocytes from apolipoprotein E-deficient (apoE-/-) mice compared with age-matched wild-type C57BL/6 (WT) mice. Experiments were performed in young (2-months) and in old (18-months) male wild-type (WT) and apoE-/- mice. Results: Besides the expected differences in serum lipid profile and plaque formation, we observed that atherosclerotic mice exhibited a significant increase in monocytosis and in serum levels of pro-inflammatory cytokines compared to WT mice. Moreover, it was observed that the overproduction of ROS, led to an increased DNA fragmentation and, consequently, apoptosis in monocytes from normocholesterolemic old mice, which was aggravated in age-matched atherosclerotic mice. Conclusions: In this study, we demonstrate that a pro-inflammatory systemic status is associated with an impairment of functionality of monocytes during aging and that these parameters are fundamental extra-arterial contributors to the aggravation of atherosclerosis. The present data open new avenues for the development of future strategies with the purpose of treating atherosclerosis.
author	Jacinto,Thais A. Meireles,Giselle S. Dias,Ananda T. Aires,Rafaela Porto,Marcella L. Gava,Agata L. Vasquez,Elisardo C. Pereira,Thiago Melo C. Campagnaro,Bianca P. Meyrelles,Silvana S.
author_facet	Jacinto,Thais A. Meireles,Giselle S. Dias,Ananda T. Aires,Rafaela Porto,Marcella L. Gava,Agata L. Vasquez,Elisardo C. Pereira,Thiago Melo C. Campagnaro,Bianca P. Meyrelles,Silvana S.
author_sort	Jacinto,Thais A.
title	Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis
title_short	Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis
title_full	Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis
title_fullStr	Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis
title_full_unstemmed	Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis
title_sort	increased ros production and dna damage in monocytes are biomarkers of aging and atherosclerosis
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100228
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Increased ROS production and DNA damage in monocytes are biomarkers of aging and atherosclerosis

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