ADAR1-mediated RNA-editing of 3′UTRs in breast cancer

Abstract Background: Whole transcriptome RNA variant analyses have shown that adenosine deaminases acting on RNA ( ADAR ) enzymes modify a large proportion of cellular RNAs, contributing to transcriptome diversity and cancer evolution. Despite the advances in the understanding of ADAR function in b...

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Autores principales: Sagredo,Eduardo A., Blanco,Alejandro, Sagredo,Alfredo I., Pérez,Paola, Sepúlveda-Hermosilla,Gonzalo, Morales,Fernanda, Müller,Bettina, Verdugo,Ricardo, Marcelain,Katherine, Harismendy,Olivier, Armisén,Ricardo
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2018
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100231
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spelling oai:scielo:S0716-976020180001002312018-12-05ADAR1-mediated RNA-editing of 3′UTRs in breast cancerSagredo,Eduardo A.Blanco,AlejandroSagredo,Alfredo I.Pérez,PaolaSepúlveda-Hermosilla,GonzaloMorales,FernandaMüller,BettinaVerdugo,RicardoMarcelain,KatherineHarismendy,OlivierArmisén,Ricardo Breast cancer ADAR1 3′UTR Editing Abstract Background: Whole transcriptome RNA variant analyses have shown that adenosine deaminases acting on RNA ( ADAR ) enzymes modify a large proportion of cellular RNAs, contributing to transcriptome diversity and cancer evolution. Despite the advances in the understanding of ADAR function in breast cancer, ADAR RNA editing functional consequences are not fully addressed. Results: We characterized A to G(I) mRNA editing in 81 breast cell lines, showing increased editing at 3′UTR and exonic regions in breast cancer cells compared to immortalized non-malignant cell lines. In addition, tumors from the BRCA TCGA cohort show a 24% increase in editing over normal breast samples when looking at 571 well-characterized UTRs targeted by ADAR1. Basal-like subtype breast cancer patients with high level of ADAR1 mRNA expression shows a worse clinical outcome and increased editing in their 3′UTRs. Interestingly, editing was particularly increased in the 3′UTRs of ATM, GINS4 and POLH transcripts in tumors, which correlated with their mRNA expression. We confirmed the role of ADAR1 in this regulation using a shRNA in a breast cancer cell line (ZR-75-1). Conclusions: Altogether, these results revealed a significant association between the mRNA editing in genes related to cancer-relevant pathways and clinical outcomes, suggesting an important role of ADAR1 expression and function in breast cancer.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100231en10.1186/s40659-018-0185-4
institution Scielo Chile
collection Scielo Chile
language English
topic Breast cancer
ADAR1
3′UTR
Editing
spellingShingle Breast cancer
ADAR1
3′UTR
Editing
Sagredo,Eduardo A.
Blanco,Alejandro
Sagredo,Alfredo I.
Pérez,Paola
Sepúlveda-Hermosilla,Gonzalo
Morales,Fernanda
Müller,Bettina
Verdugo,Ricardo
Marcelain,Katherine
Harismendy,Olivier
Armisén,Ricardo
ADAR1-mediated RNA-editing of 3′UTRs in breast cancer
description Abstract Background: Whole transcriptome RNA variant analyses have shown that adenosine deaminases acting on RNA ( ADAR ) enzymes modify a large proportion of cellular RNAs, contributing to transcriptome diversity and cancer evolution. Despite the advances in the understanding of ADAR function in breast cancer, ADAR RNA editing functional consequences are not fully addressed. Results: We characterized A to G(I) mRNA editing in 81 breast cell lines, showing increased editing at 3′UTR and exonic regions in breast cancer cells compared to immortalized non-malignant cell lines. In addition, tumors from the BRCA TCGA cohort show a 24% increase in editing over normal breast samples when looking at 571 well-characterized UTRs targeted by ADAR1. Basal-like subtype breast cancer patients with high level of ADAR1 mRNA expression shows a worse clinical outcome and increased editing in their 3′UTRs. Interestingly, editing was particularly increased in the 3′UTRs of ATM, GINS4 and POLH transcripts in tumors, which correlated with their mRNA expression. We confirmed the role of ADAR1 in this regulation using a shRNA in a breast cancer cell line (ZR-75-1). Conclusions: Altogether, these results revealed a significant association between the mRNA editing in genes related to cancer-relevant pathways and clinical outcomes, suggesting an important role of ADAR1 expression and function in breast cancer.
author Sagredo,Eduardo A.
Blanco,Alejandro
Sagredo,Alfredo I.
Pérez,Paola
Sepúlveda-Hermosilla,Gonzalo
Morales,Fernanda
Müller,Bettina
Verdugo,Ricardo
Marcelain,Katherine
Harismendy,Olivier
Armisén,Ricardo
author_facet Sagredo,Eduardo A.
Blanco,Alejandro
Sagredo,Alfredo I.
Pérez,Paola
Sepúlveda-Hermosilla,Gonzalo
Morales,Fernanda
Müller,Bettina
Verdugo,Ricardo
Marcelain,Katherine
Harismendy,Olivier
Armisén,Ricardo
author_sort Sagredo,Eduardo A.
title ADAR1-mediated RNA-editing of 3′UTRs in breast cancer
title_short ADAR1-mediated RNA-editing of 3′UTRs in breast cancer
title_full ADAR1-mediated RNA-editing of 3′UTRs in breast cancer
title_fullStr ADAR1-mediated RNA-editing of 3′UTRs in breast cancer
title_full_unstemmed ADAR1-mediated RNA-editing of 3′UTRs in breast cancer
title_sort adar1-mediated rna-editing of 3′utrs in breast cancer
publisher Sociedad de Biología de Chile
publishDate 2018
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100231
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