MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2

Abstract Background: Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncodin...

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Autores principales:	Xing,Feng, Song,Zhijiao, He,Yuanying
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	Ovarian cancer miR-219-5p HMGA2 Growth Metastasis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100240
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spelling	oai:scielo:S0716-976020180001002402019-06-24MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2Xing,FengSong,ZhijiaoHe,Yuanying Ovarian cancer miR-219-5p HMGA2 Growth Metastasis Abstract Background: Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncoding RNAs, negatively regulate gene expression and their dysregulation has been implicated in tumorigenesis. The aim of this study was to investigate the potential involvement of a specific miRNA, miR-219-5p, in HMGA2-induced ovarian cancer. Methods: The ovarian cancer cell line, SKOV3, was employed, and miR-219-5p and HMGA2 overexpression vectors constructed. The CCK-8 kit was used to determine cell proliferation and the Transwell® assay used to measure cell invasion and migration. RT-PCR and western blot analyses were applied to analyze the expression of miR-219-5p and HMGA2, and the luciferase reporter assay used to examine the interactions between miR-219-5p and HMGA2. Nude mice were employed to characterize in vivo tumor growth regulation. Results: Expression of miR-219-5p led to suppression of proliferation, invasion and migration of the ovarian cancer cell line, SKOV3, by targeting HMGA2. The inhibitory effects of miR-219-5p were reversed upon overexpression of HMGA2. Data from the luciferase reporter assay showed that miR-219-5p downregulates HMGA2 via direct integration with its 3’-UTR. Consistent with in vitro findings, expression of miR-219-5p led to significant inhibition of tumor growth in vivo. Conclusion: Our results collectively suggest that miR-219-5p inhibits tumor growth and metastasis by targeting HMGA2.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100240en10.1186/s40659-018-0199-y
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ovarian cancer miR-219-5p HMGA2 Growth Metastasis
spellingShingle	Ovarian cancer miR-219-5p HMGA2 Growth Metastasis Xing,Feng Song,Zhijiao He,Yuanying MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2
description	Abstract Background: Accumulating studies have demonstrated that high-mobility group A2 (HMGA2), an oncofetal protein, plays a role in tumor development and progression. However, the molecular role of HMGA2 in ovarian carcinoma is yet to be established. MicroRNAs (miRNAs), a group of small noncoding RNAs, negatively regulate gene expression and their dysregulation has been implicated in tumorigenesis. The aim of this study was to investigate the potential involvement of a specific miRNA, miR-219-5p, in HMGA2-induced ovarian cancer. Methods: The ovarian cancer cell line, SKOV3, was employed, and miR-219-5p and HMGA2 overexpression vectors constructed. The CCK-8 kit was used to determine cell proliferation and the Transwell® assay used to measure cell invasion and migration. RT-PCR and western blot analyses were applied to analyze the expression of miR-219-5p and HMGA2, and the luciferase reporter assay used to examine the interactions between miR-219-5p and HMGA2. Nude mice were employed to characterize in vivo tumor growth regulation. Results: Expression of miR-219-5p led to suppression of proliferation, invasion and migration of the ovarian cancer cell line, SKOV3, by targeting HMGA2. The inhibitory effects of miR-219-5p were reversed upon overexpression of HMGA2. Data from the luciferase reporter assay showed that miR-219-5p downregulates HMGA2 via direct integration with its 3’-UTR. Consistent with in vitro findings, expression of miR-219-5p led to significant inhibition of tumor growth in vivo. Conclusion: Our results collectively suggest that miR-219-5p inhibits tumor growth and metastasis by targeting HMGA2.
author	Xing,Feng Song,Zhijiao He,Yuanying
author_facet	Xing,Feng Song,Zhijiao He,Yuanying
author_sort	Xing,Feng
title	MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2
title_short	MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2
title_full	MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2
title_fullStr	MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2
title_full_unstemmed	MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2
title_sort	mir-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting hmga2
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100240
work_keys_str_mv	AT xingfeng mir2195pinhibitsgrowthandmetastasisofovariancancercellsbytargetinghmga2 AT songzhijiao mir2195pinhibitsgrowthandmetastasisofovariancancercellsbytargetinghmga2 AT heyuanying mir2195pinhibitsgrowthandmetastasisofovariancancercellsbytargetinghmga2
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MiR-219-5p inhibits growth and metastasis of ovarian cancer cells by targeting HMGA2

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