MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1

Abstract Background: Emerging evidence showed that microRNAs (miRs) play critical roles in human cancers by functioning as either tumor suppressor or oncogene. MIR-382 was found to function as tumor suppressor in certain cancers. However, the role of MIR-382 in colorectal cancer (CRC) is largely un...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ren,Yupeng, Zhang,Hao, Jiang,Peng
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	MIR-382 SP1 Colorectal cancer Tumor progression
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100241
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602018000100241
record_format	dspace
spelling	oai:scielo:S0716-976020180001002412019-06-24MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1Ren,YupengZhang,HaoJiang,Peng MIR-382 SP1 Colorectal cancer Tumor progression Abstract Background: Emerging evidence showed that microRNAs (miRs) play critical roles in human cancers by functioning as either tumor suppressor or oncogene. MIR-382 was found to function as tumor suppressor in certain cancers. However, the role of MIR-382 in colorectal cancer (CRC) is largely unknown. Specificity protein 1 (SP1) is highly expressed in several cancers including CRC and is correlated with poor prognosis, but it is unclear whether or not MIR-382 can regulate the expression of SP1. Methods: MIR-382 expression level was measured by reverse transcription-quantitative polymerase chain reaction. The connection between MIR-382 and SP1 was validated by luciferase activity reporter assay and western blot assay. Cell counting kit-8 assay and wound-healing assay were conducted to investigate the biological functions of MIR-382 in CRC. Results: In this study, we found MIR-382 expression was downregulated in CRC tissues and cell lines, and the transfection of MIR-382 mimic decreased cell growth and migration. Furthermore, we identified SP1 was a direct target of MIR-382. Overexpression of MIR-382 decreased the expression of SP1, whereas MIR-382 knockdown promoted SP1 expression. We also observed an inversely correlation between MIR-382 and SP1 in CRC tissues. Additionally, we showed that knockdown of SP1 inhibited cell growth and migration and attenuated the effect of MIR-382 inhibitor on cell behaviors. Conclusions: In conclusion, the present study describes a potential mechanism underlying a MIR-382/SP1 link contributing to CRC development. Thus, MIR-382 may be able to be developed as a novel treatment target for CRC.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100241en10.1186/s40659-018-0200-9
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	MIR-382 SP1 Colorectal cancer Tumor progression
spellingShingle	MIR-382 SP1 Colorectal cancer Tumor progression Ren,Yupeng Zhang,Hao Jiang,Peng MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1
description	Abstract Background: Emerging evidence showed that microRNAs (miRs) play critical roles in human cancers by functioning as either tumor suppressor or oncogene. MIR-382 was found to function as tumor suppressor in certain cancers. However, the role of MIR-382 in colorectal cancer (CRC) is largely unknown. Specificity protein 1 (SP1) is highly expressed in several cancers including CRC and is correlated with poor prognosis, but it is unclear whether or not MIR-382 can regulate the expression of SP1. Methods: MIR-382 expression level was measured by reverse transcription-quantitative polymerase chain reaction. The connection between MIR-382 and SP1 was validated by luciferase activity reporter assay and western blot assay. Cell counting kit-8 assay and wound-healing assay were conducted to investigate the biological functions of MIR-382 in CRC. Results: In this study, we found MIR-382 expression was downregulated in CRC tissues and cell lines, and the transfection of MIR-382 mimic decreased cell growth and migration. Furthermore, we identified SP1 was a direct target of MIR-382. Overexpression of MIR-382 decreased the expression of SP1, whereas MIR-382 knockdown promoted SP1 expression. We also observed an inversely correlation between MIR-382 and SP1 in CRC tissues. Additionally, we showed that knockdown of SP1 inhibited cell growth and migration and attenuated the effect of MIR-382 inhibitor on cell behaviors. Conclusions: In conclusion, the present study describes a potential mechanism underlying a MIR-382/SP1 link contributing to CRC development. Thus, MIR-382 may be able to be developed as a novel treatment target for CRC.
author	Ren,Yupeng Zhang,Hao Jiang,Peng
author_facet	Ren,Yupeng Zhang,Hao Jiang,Peng
author_sort	Ren,Yupeng
title	MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1
title_short	MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1
title_full	MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1
title_fullStr	MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1
title_full_unstemmed	MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1
title_sort	microrna-382 inhibits cell growth and migration in colorectal cancer by targeting sp1
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100241
work_keys_str_mv	AT renyupeng microrna382inhibitscellgrowthandmigrationincolorectalcancerbytargetingsp1 AT zhanghao microrna382inhibitscellgrowthandmigrationincolorectalcancerbytargetingsp1 AT jiangpeng microrna382inhibitscellgrowthandmigrationincolorectalcancerbytargetingsp1
_version_	1718441578462183424

MicroRNA-382 inhibits cell growth and migration in colorectal cancer by targeting SP1

Ejemplares similares