Overexpression of PIK3R1 promotes hepatocellular carcinoma progression

Abstract Background: Phosphoinositide-3-kinase, regulatory subunit 1 (PIK3R1) could regulate cancer cell proliferation important for cancer cell proliferation; however, its role in Hepatocellular carcinoma (HCC) remains largely unknown. Here, we investigated the role of PIK3R1 in HCC and examined t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ai,Xuejun, Xiang,Lei, Huang,Zhi, Zhou,Shi, Zhang,Shuai, Zhang,Tao, Jiang,Tianpeng
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	Hepatocellular carcinoma PIK3R1 Apoptosis Targeted therapy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100242
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602018000100242
record_format	dspace
spelling	oai:scielo:S0716-976020180001002422019-06-24Overexpression of PIK3R1 promotes hepatocellular carcinoma progressionAi,XuejunXiang,LeiHuang,ZhiZhou,ShiZhang,ShuaiZhang,TaoJiang,Tianpeng Hepatocellular carcinoma PIK3R1 Apoptosis Targeted therapy Abstract Background: Phosphoinositide-3-kinase, regulatory subunit 1 (PIK3R1) could regulate cancer cell proliferation important for cancer cell proliferation; however, its role in Hepatocellular carcinoma (HCC) remains largely unknown. Here, we investigated the role of PIK3R1 in HCC and examined the underlying molecular mechanisms. Methods: The expression of PIK3R1 was evaluated by immunohistochemistry and qRT-PCR in a series of HCC tissues. The mRNA and protein expression of PIK3R1 was used by qRT-PCR and western blot assays in a series of human HCC cell lines, and then we choose MHCC97H and HCCLM3 cells as a model to investigate the effect of PIK3R1 on HCC progression. The effects of PIK3R1 knowdown on cell proliferation, migration, apoptosis of HCC were assessed by the MTT assay, clonogenic assays, wound healing assay and flow cytometry in vitro. Western blot assay was performed to assess the expression changes of PI3K/AKT/mTOR signaling pathway. Results: Our results found that PIK3R1 was highly expressed in HCC tissues compared with adjacent normal tissues. Knockdown of PIK3R1 inhibited the proliferation, migration and promoted apoptosis of HCC cell lines. In addition, we proved that knockdown of PIK3R1 downregulated p-PI3K, p-AKT, and p-mTOR expressions in MHCC97H and HCCLM3 cells. Conclusions: In conclusion, PIK3R1 providing potential novel targets for the treatment of HCC.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100242en10.1186/s40659-018-0202-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Hepatocellular carcinoma PIK3R1 Apoptosis Targeted therapy
spellingShingle	Hepatocellular carcinoma PIK3R1 Apoptosis Targeted therapy Ai,Xuejun Xiang,Lei Huang,Zhi Zhou,Shi Zhang,Shuai Zhang,Tao Jiang,Tianpeng Overexpression of PIK3R1 promotes hepatocellular carcinoma progression
description	Abstract Background: Phosphoinositide-3-kinase, regulatory subunit 1 (PIK3R1) could regulate cancer cell proliferation important for cancer cell proliferation; however, its role in Hepatocellular carcinoma (HCC) remains largely unknown. Here, we investigated the role of PIK3R1 in HCC and examined the underlying molecular mechanisms. Methods: The expression of PIK3R1 was evaluated by immunohistochemistry and qRT-PCR in a series of HCC tissues. The mRNA and protein expression of PIK3R1 was used by qRT-PCR and western blot assays in a series of human HCC cell lines, and then we choose MHCC97H and HCCLM3 cells as a model to investigate the effect of PIK3R1 on HCC progression. The effects of PIK3R1 knowdown on cell proliferation, migration, apoptosis of HCC were assessed by the MTT assay, clonogenic assays, wound healing assay and flow cytometry in vitro. Western blot assay was performed to assess the expression changes of PI3K/AKT/mTOR signaling pathway. Results: Our results found that PIK3R1 was highly expressed in HCC tissues compared with adjacent normal tissues. Knockdown of PIK3R1 inhibited the proliferation, migration and promoted apoptosis of HCC cell lines. In addition, we proved that knockdown of PIK3R1 downregulated p-PI3K, p-AKT, and p-mTOR expressions in MHCC97H and HCCLM3 cells. Conclusions: In conclusion, PIK3R1 providing potential novel targets for the treatment of HCC.
author	Ai,Xuejun Xiang,Lei Huang,Zhi Zhou,Shi Zhang,Shuai Zhang,Tao Jiang,Tianpeng
author_facet	Ai,Xuejun Xiang,Lei Huang,Zhi Zhou,Shi Zhang,Shuai Zhang,Tao Jiang,Tianpeng
author_sort	Ai,Xuejun
title	Overexpression of PIK3R1 promotes hepatocellular carcinoma progression
title_short	Overexpression of PIK3R1 promotes hepatocellular carcinoma progression
title_full	Overexpression of PIK3R1 promotes hepatocellular carcinoma progression
title_fullStr	Overexpression of PIK3R1 promotes hepatocellular carcinoma progression
title_full_unstemmed	Overexpression of PIK3R1 promotes hepatocellular carcinoma progression
title_sort	overexpression of pik3r1 promotes hepatocellular carcinoma progression
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100242
work_keys_str_mv	AT aixuejun overexpressionofpik3r1promoteshepatocellularcarcinomaprogression AT xianglei overexpressionofpik3r1promoteshepatocellularcarcinomaprogression AT huangzhi overexpressionofpik3r1promoteshepatocellularcarcinomaprogression AT zhoushi overexpressionofpik3r1promoteshepatocellularcarcinomaprogression AT zhangshuai overexpressionofpik3r1promoteshepatocellularcarcinomaprogression AT zhangtao overexpressionofpik3r1promoteshepatocellularcarcinomaprogression AT jiangtianpeng overexpressionofpik3r1promoteshepatocellularcarcinomaprogression
_version_	1718441578763124736

Overexpression of PIK3R1 promotes hepatocellular carcinoma progression

Ejemplares similares