The deubiquitinase USP38 affects cellular functions through interacting with LSD1

Abstract Background: Deubiquitination is a posttranslational protein modification prevalent in mammalian cells. Deubiquitinases regulate the functions of the target protein by removing its ubiquitin chain. In this study, the effects of the deubiquitinase USP38's functions on the LSD1 protein a...

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Autores principales:	Liu,Wenbin, Zhang,Qi, Fang,Yuanyuan, Wang,Yanan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	LSD1 Deubiquitinase USP38 Proliferation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100243
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spelling	oai:scielo:S0716-976020180001002432019-06-24The deubiquitinase USP38 affects cellular functions through interacting with LSD1Liu,WenbinZhang,QiFang,YuanyuanWang,Yanan LSD1 Deubiquitinase USP38 Proliferation Abstract Background: Deubiquitination is a posttranslational protein modification prevalent in mammalian cells. Deubiquitinases regulate the functions of the target protein by removing its ubiquitin chain. In this study, the effects of the deubiquitinase USP38's functions on the LSD1 protein and on cell physiology were investigated. Materials and methods: Western blotting, real-time quantitative PCR, immunoprecipitation, denaturing immunoprecipitation and luciferase reporter assays were used to analyze the protein stability, protein interactions and changes in the ubiquitin chain. Cell proliferation assays, colony formation assays, drug treatments and western blotting were used to explore the functions of USP38 in cells. Results: The deubiquitinase USP38 stabilizes protein LSD1 in cells by binding LSD1 and cleaving its ubiquitin chain to prevent the degradation of LSD1 by the intracellular proteasome. USP38 enhances the ability of LSD1 to activate signaling pathways and hence promotes cellular abilities of proliferation and colony formation through interacting with LSD1. Furthermore, USP38 enhances the drug tolerance of human colon cancer cells. Conclusions: USP38 is an LSD1-specific deubiquitinase that affects cellular physiology through interacting with LSD1.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100243en10.1186/s40659-018-0201-8
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	LSD1 Deubiquitinase USP38 Proliferation
spellingShingle	LSD1 Deubiquitinase USP38 Proliferation Liu,Wenbin Zhang,Qi Fang,Yuanyuan Wang,Yanan The deubiquitinase USP38 affects cellular functions through interacting with LSD1
description	Abstract Background: Deubiquitination is a posttranslational protein modification prevalent in mammalian cells. Deubiquitinases regulate the functions of the target protein by removing its ubiquitin chain. In this study, the effects of the deubiquitinase USP38's functions on the LSD1 protein and on cell physiology were investigated. Materials and methods: Western blotting, real-time quantitative PCR, immunoprecipitation, denaturing immunoprecipitation and luciferase reporter assays were used to analyze the protein stability, protein interactions and changes in the ubiquitin chain. Cell proliferation assays, colony formation assays, drug treatments and western blotting were used to explore the functions of USP38 in cells. Results: The deubiquitinase USP38 stabilizes protein LSD1 in cells by binding LSD1 and cleaving its ubiquitin chain to prevent the degradation of LSD1 by the intracellular proteasome. USP38 enhances the ability of LSD1 to activate signaling pathways and hence promotes cellular abilities of proliferation and colony formation through interacting with LSD1. Furthermore, USP38 enhances the drug tolerance of human colon cancer cells. Conclusions: USP38 is an LSD1-specific deubiquitinase that affects cellular physiology through interacting with LSD1.
author	Liu,Wenbin Zhang,Qi Fang,Yuanyuan Wang,Yanan
author_facet	Liu,Wenbin Zhang,Qi Fang,Yuanyuan Wang,Yanan
author_sort	Liu,Wenbin
title	The deubiquitinase USP38 affects cellular functions through interacting with LSD1
title_short	The deubiquitinase USP38 affects cellular functions through interacting with LSD1
title_full	The deubiquitinase USP38 affects cellular functions through interacting with LSD1
title_fullStr	The deubiquitinase USP38 affects cellular functions through interacting with LSD1
title_full_unstemmed	The deubiquitinase USP38 affects cellular functions through interacting with LSD1
title_sort	deubiquitinase usp38 affects cellular functions through interacting with lsd1
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100243
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The deubiquitinase USP38 affects cellular functions through interacting with LSD1

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