Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation

Abstract Background: Hyperpigmentation disorders such as post-inflammatory hyperpigmentation are major concerns not only in light-skinned people but also in Asian populations with darker skin. The anti-tyrosinase and immunomodulatory effects of sericin have been known for decades. However, the ther...

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Autores principales:	Aramwit,Pornanong, Luplertlop,Natthanej, Kanjanapruthipong,Tapanee, Ampawong,Sumate
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	Antityrosinase Melanogenesis MITF Post inflammatory hyperpigmentation Sericin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100244
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spelling	oai:scielo:S0716-976020180001002442019-06-24Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentationAramwit,PornanongLuplertlop,NatthanejKanjanapruthipong,TapaneeAmpawong,Sumate Antityrosinase Melanogenesis MITF Post inflammatory hyperpigmentation Sericin Abstract Background: Hyperpigmentation disorders such as post-inflammatory hyperpigmentation are major concerns not only in light-skinned people but also in Asian populations with darker skin. The anti-tyrosinase and immunomodulatory effects of sericin have been known for decades. However, the therapeutic effects of sericin on hyperpigmentation disorders have not been well documented. Methods: In this study, we used an in vitro model to study the anti-tyrosinase, tolerogenic, and anti-melanogenic effects of sericin on Staphylococcus aureus peptidoglycan (PEG)-stimulated melanocytes, dendritic cells (DCs), and artificial skin (MelanoDerm™). Enzyme-linked immunosorbent assay, conventional and immunolabeled electron microscopy, and histopathological studies were performed. Results: The results revealed that urea-extracted sericin has strong anti-tyrosinase properties as shown by a reduction of tyrosinase activity in melanin pigments both 48 h and 10 days after allergic induction with PEG. Anti-inflammatory cytokines including interleukin (IL)-4, IL-10, and transforming growth factor-p were upregulated upon sericin treatment (10, 20, and 50 μg/mL), whereas production of allergic chemokines, CCL8 and CCL18, by DCs was diminished 48 h after allergic induction with PEG. Moreover, sericin lowered the expression of micropthalmia-associated transcription factor (MITF), a marker of melanogenesis regulation, in melanocytes and keratinocytes, which contributed to the reduction of melanin size and the magnitude of melanin deposition. However, sericin had no effect on melanin transport between melanocytes and keratinocytes, as demonstrated by a high retention of cytoskeletal components. Conclusion: In summary, sericin suppresses melanogenesis by inhibition of tyrosinase activity, reduction of inflammation and allergy, and modulation of MITF function.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100244en10.1186/s40659-018-0204-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antityrosinase Melanogenesis MITF Post inflammatory hyperpigmentation Sericin
spellingShingle	Antityrosinase Melanogenesis MITF Post inflammatory hyperpigmentation Sericin Aramwit,Pornanong Luplertlop,Natthanej Kanjanapruthipong,Tapanee Ampawong,Sumate Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation
description	Abstract Background: Hyperpigmentation disorders such as post-inflammatory hyperpigmentation are major concerns not only in light-skinned people but also in Asian populations with darker skin. The anti-tyrosinase and immunomodulatory effects of sericin have been known for decades. However, the therapeutic effects of sericin on hyperpigmentation disorders have not been well documented. Methods: In this study, we used an in vitro model to study the anti-tyrosinase, tolerogenic, and anti-melanogenic effects of sericin on Staphylococcus aureus peptidoglycan (PEG)-stimulated melanocytes, dendritic cells (DCs), and artificial skin (MelanoDerm™). Enzyme-linked immunosorbent assay, conventional and immunolabeled electron microscopy, and histopathological studies were performed. Results: The results revealed that urea-extracted sericin has strong anti-tyrosinase properties as shown by a reduction of tyrosinase activity in melanin pigments both 48 h and 10 days after allergic induction with PEG. Anti-inflammatory cytokines including interleukin (IL)-4, IL-10, and transforming growth factor-p were upregulated upon sericin treatment (10, 20, and 50 μg/mL), whereas production of allergic chemokines, CCL8 and CCL18, by DCs was diminished 48 h after allergic induction with PEG. Moreover, sericin lowered the expression of micropthalmia-associated transcription factor (MITF), a marker of melanogenesis regulation, in melanocytes and keratinocytes, which contributed to the reduction of melanin size and the magnitude of melanin deposition. However, sericin had no effect on melanin transport between melanocytes and keratinocytes, as demonstrated by a high retention of cytoskeletal components. Conclusion: In summary, sericin suppresses melanogenesis by inhibition of tyrosinase activity, reduction of inflammation and allergy, and modulation of MITF function.
author	Aramwit,Pornanong Luplertlop,Natthanej Kanjanapruthipong,Tapanee Ampawong,Sumate
author_facet	Aramwit,Pornanong Luplertlop,Natthanej Kanjanapruthipong,Tapanee Ampawong,Sumate
author_sort	Aramwit,Pornanong
title	Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation
title_short	Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation
title_full	Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation
title_fullStr	Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation
title_full_unstemmed	Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation
title_sort	effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100244
work_keys_str_mv	AT aramwitpornanong effectofureaextractedsericinonmelanogenesispotentialapplicationsinpostinflammatoryhyperpigmentation AT luplertlopnatthanej effectofureaextractedsericinonmelanogenesispotentialapplicationsinpostinflammatoryhyperpigmentation AT kanjanapruthipongtapanee effectofureaextractedsericinonmelanogenesispotentialapplicationsinpostinflammatoryhyperpigmentation AT ampawongsumate effectofureaextractedsericinonmelanogenesispotentialapplicationsinpostinflammatoryhyperpigmentation
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Effect of urea-extracted sericin on melanogenesis: potential applications in post-inflammatory hyperpigmentation

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