Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)
Abstract Background: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to...
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| Lenguaje: | English |
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Sociedad de Biología de Chile
2018
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oai:scielo:S0716-976020180001004022018-12-05Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae)Garcia-Gonzales,RolandoPico-Mendoza,JoséQuiroz,KarlaCarrasco,BasilioCáceres,PabloChong-Perez,BorysPino,HugoSeiltgens,MarjorieGreck,EglisCaligari,Peter D. S. Microsatellite Pyrosequencing Chilean berry Gaultheria pumila Abstract Background: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. Results: Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. Conclusions: From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100402en10.1186/s40659-018-0193-4 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Microsatellite Pyrosequencing Chilean berry Gaultheria pumila |
| spellingShingle |
Microsatellite Pyrosequencing Chilean berry Gaultheria pumila Garcia-Gonzales,Rolando Pico-Mendoza,José Quiroz,Karla Carrasco,Basilio Cáceres,Pablo Chong-Perez,Borys Pino,Hugo Seiltgens,Marjorie Greck,Eglis Caligari,Peter D. S. Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae) |
| description |
Abstract Background: Polymorphic microsatellite markers were developed for Gaultheria pumila (Ericaceae) to evaluate genetic diversity and population structure within its native range in Chile. This is a very important Ericaceae endemic to Chile with a large commercial potential. Its resistance to different abiotic conditions makes it a valuable target for genetic improvement. Results: Ten polymorphic simple sequence repeat (SSR) loci were isolated from Gaultheria pumila using new-generation 454 FLX Titanium pyrosequencing technology. The mean number of alleles per locus ranged from 2 to 4. Observed and expected heterozygosity ranged from 0.00 to 1.0 and 0.00 to 0.64, respectively. Conclusions: From 10 SSR markers developed for G. pumila, 9 markers are promising candidates for analyzing genetic variation within or between natural populations of G. pumila and other species from the same genus. |
| author |
Garcia-Gonzales,Rolando Pico-Mendoza,José Quiroz,Karla Carrasco,Basilio Cáceres,Pablo Chong-Perez,Borys Pino,Hugo Seiltgens,Marjorie Greck,Eglis Caligari,Peter D. S. |
| author_facet |
Garcia-Gonzales,Rolando Pico-Mendoza,José Quiroz,Karla Carrasco,Basilio Cáceres,Pablo Chong-Perez,Borys Pino,Hugo Seiltgens,Marjorie Greck,Eglis Caligari,Peter D. S. |
| author_sort |
Garcia-Gonzales,Rolando |
| title |
Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae) |
| title_short |
Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae) |
| title_full |
Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae) |
| title_fullStr |
Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae) |
| title_full_unstemmed |
Development and characterization of microsatellite markers in Gaultheria pumila Lf. (Ericaceae) |
| title_sort |
development and characterization of microsatellite markers in gaultheria pumila lf. (ericaceae) |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2018 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100402 |
| work_keys_str_mv |
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| _version_ |
1718441580969328640 |