Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance

Abstract Background: Drought is a major abiotic stress affecting global wheat (Triticum aestivum L.) production. Exploration of drought-tolerant genes is essential for the genetic improvement of drought tolerance in wheat. Previous studies have shown that some histone encoding genes are involved in...

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Autores principales:	Wang,Xinbo, Ren,Yongzhe, Li,Jingjing, Wang,Zhiqiang, Xin,Zeyu, Lin,Tongbao
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	Drought Triticum aestivum L. TaH2B-7D Knock-down
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100212
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spelling	oai:scielo:S0716-976020190001002122019-10-10Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought toleranceWang,XinboRen,YongzheLi,JingjingWang,ZhiqiangXin,ZeyuLin,Tongbao Drought Triticum aestivum L. TaH2B-7D Knock-down Abstract Background: Drought is a major abiotic stress affecting global wheat (Triticum aestivum L.) production. Exploration of drought-tolerant genes is essential for the genetic improvement of drought tolerance in wheat. Previous studies have shown that some histone encoding genes are involved in plant drought tolerance. However, whether the H2B family genes are involved in drought stress response remains unclear. Methods: Here, we identified a wheat histone H2B family gene, TaH2B-7D, which was significantly up-regulated under drought stress conditions. Virus-induced gene silencing (VIGS) technology was used to further verify the function of TaH2B-7D in wheat drought tolerance. The phenotypic and physiological changes were examined in the TaH2B-7D knock-down plants. Results: In the TaH2B-7D knock-down plants, relative electrolyte leakage rate and malonaldehyde (MDA) content significantly increased, while relative water content (RWC) and proline content significantly decreased compared with those in the non-knocked-down plants under drought stress conditions. TaH2B-7D knock-down plants exhibited severe sagging, wilting and dwarf phenotypes under drought stress conditions, but not in the non-knocked-down plants, suggesting that the former were more sensitive to drought stress. Conclusion: These results indicate that TaH2B-7D potentially plays a vital role in conferring drought tolerance in wheat.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100212en10.1186/s40659-019-0222-y
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Drought Triticum aestivum L. TaH2B-7D Knock-down
spellingShingle	Drought Triticum aestivum L. TaH2B-7D Knock-down Wang,Xinbo Ren,Yongzhe Li,Jingjing Wang,Zhiqiang Xin,Zeyu Lin,Tongbao Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance
description	Abstract Background: Drought is a major abiotic stress affecting global wheat (Triticum aestivum L.) production. Exploration of drought-tolerant genes is essential for the genetic improvement of drought tolerance in wheat. Previous studies have shown that some histone encoding genes are involved in plant drought tolerance. However, whether the H2B family genes are involved in drought stress response remains unclear. Methods: Here, we identified a wheat histone H2B family gene, TaH2B-7D, which was significantly up-regulated under drought stress conditions. Virus-induced gene silencing (VIGS) technology was used to further verify the function of TaH2B-7D in wheat drought tolerance. The phenotypic and physiological changes were examined in the TaH2B-7D knock-down plants. Results: In the TaH2B-7D knock-down plants, relative electrolyte leakage rate and malonaldehyde (MDA) content significantly increased, while relative water content (RWC) and proline content significantly decreased compared with those in the non-knocked-down plants under drought stress conditions. TaH2B-7D knock-down plants exhibited severe sagging, wilting and dwarf phenotypes under drought stress conditions, but not in the non-knocked-down plants, suggesting that the former were more sensitive to drought stress. Conclusion: These results indicate that TaH2B-7D potentially plays a vital role in conferring drought tolerance in wheat.
author	Wang,Xinbo Ren,Yongzhe Li,Jingjing Wang,Zhiqiang Xin,Zeyu Lin,Tongbao
author_facet	Wang,Xinbo Ren,Yongzhe Li,Jingjing Wang,Zhiqiang Xin,Zeyu Lin,Tongbao
author_sort	Wang,Xinbo
title	Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance
title_short	Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance
title_full	Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance
title_fullStr	Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance
title_full_unstemmed	Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance
title_sort	knock-down the expression of tah2b-7d using virus-induced gene silencing reduces wheat drought tolerance
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100212
work_keys_str_mv	AT wangxinbo knockdowntheexpressionoftah2b7dusingvirusinducedgenesilencingreduceswheatdroughttolerance AT renyongzhe knockdowntheexpressionoftah2b7dusingvirusinducedgenesilencingreduceswheatdroughttolerance AT lijingjing knockdowntheexpressionoftah2b7dusingvirusinducedgenesilencingreduceswheatdroughttolerance AT wangzhiqiang knockdowntheexpressionoftah2b7dusingvirusinducedgenesilencingreduceswheatdroughttolerance AT xinzeyu knockdowntheexpressionoftah2b7dusingvirusinducedgenesilencingreduceswheatdroughttolerance AT lintongbao knockdowntheexpressionoftah2b7dusingvirusinducedgenesilencingreduceswheatdroughttolerance
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Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance

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