MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2

Abstract Background: MicroRNAs (miRNAs) have emerged as the critical modulators of the tumorigenesis and tumor progression. Methods: The levels of miR-663 in ovarian cancer cell lines and clinical tissues were detected using qRT-PCR assays. The Transwell invasion and wound healing assay were condu...

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Autores principales:	Xie,Hui Hui, Huan,Wen Ting, Han,Jiang Qiong, Ren,Wei Ru, Yang,Li Hua
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	MiR-663 Ovarian cancer TUSC2 Growth Migration Invasion
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100216
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spelling	oai:scielo:S0716-976020190001002162019-10-10MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2Xie,Hui HuiHuan,Wen TingHan,Jiang QiongRen,Wei RuYang,Li Hua MiR-663 Ovarian cancer TUSC2 Growth Migration Invasion Abstract Background: MicroRNAs (miRNAs) have emerged as the critical modulators of the tumorigenesis and tumor progression. Methods: The levels of miR-663 in ovarian cancer cell lines and clinical tissues were detected using qRT-PCR assays. The Transwell invasion and wound healing assay were conducted to assess the roles of miR-663 in the migration and invasion of ovarian cancer cell in vitro. Rescue assays were carried out to confirm the contribution of tumor suppressor candidate 2 (TUSC2) in the aggressiveness of cancer cell which was regulated by miR-663. Results: The levels of miR-663 were up-regulated in ovarian cancer tissues in comparison with the corresponding normal tissues. Up-regulation of miR-663 increased the proliferation, colony formation, migration and invasion of ovarian cancer SKOV3 cell. Additional, over-expression of miR-663 increased the tumor growth of SKOV3 in xenograft model. Bioinformatics analysis and luciferase reporter assay identified that miR-663 decreased the level of TUSC2 via binding to the 3'-UTR of TUSC2 gene. Finally, the expression of TUSC2 was inversely associated with the level of miR–663 in ovarian carcinoma tissue and over-expression of TUSC2 inhibited the migration and invasion abilities of SKOV3 that was promoted by miR-663. Conclusion: Altogether, these results indicate that miR-663 acts as a potential tumor-promoting miRNA through targeting TUSC2 in ovarian cancer.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100216en10.1186/s40659-019-0219-6
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	MiR-663 Ovarian cancer TUSC2 Growth Migration Invasion
spellingShingle	MiR-663 Ovarian cancer TUSC2 Growth Migration Invasion Xie,Hui Hui Huan,Wen Ting Han,Jiang Qiong Ren,Wei Ru Yang,Li Hua MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2
description	Abstract Background: MicroRNAs (miRNAs) have emerged as the critical modulators of the tumorigenesis and tumor progression. Methods: The levels of miR-663 in ovarian cancer cell lines and clinical tissues were detected using qRT-PCR assays. The Transwell invasion and wound healing assay were conducted to assess the roles of miR-663 in the migration and invasion of ovarian cancer cell in vitro. Rescue assays were carried out to confirm the contribution of tumor suppressor candidate 2 (TUSC2) in the aggressiveness of cancer cell which was regulated by miR-663. Results: The levels of miR-663 were up-regulated in ovarian cancer tissues in comparison with the corresponding normal tissues. Up-regulation of miR-663 increased the proliferation, colony formation, migration and invasion of ovarian cancer SKOV3 cell. Additional, over-expression of miR-663 increased the tumor growth of SKOV3 in xenograft model. Bioinformatics analysis and luciferase reporter assay identified that miR-663 decreased the level of TUSC2 via binding to the 3'-UTR of TUSC2 gene. Finally, the expression of TUSC2 was inversely associated with the level of miR–663 in ovarian carcinoma tissue and over-expression of TUSC2 inhibited the migration and invasion abilities of SKOV3 that was promoted by miR-663. Conclusion: Altogether, these results indicate that miR-663 acts as a potential tumor-promoting miRNA through targeting TUSC2 in ovarian cancer.
author	Xie,Hui Hui Huan,Wen Ting Han,Jiang Qiong Ren,Wei Ru Yang,Li Hua
author_facet	Xie,Hui Hui Huan,Wen Ting Han,Jiang Qiong Ren,Wei Ru Yang,Li Hua
author_sort	Xie,Hui Hui
title	MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2
title_short	MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2
title_full	MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2
title_fullStr	MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2
title_full_unstemmed	MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2
title_sort	microrna-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting tusc2
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100216
work_keys_str_mv	AT xiehuihui microrna663facilitatesthegrowthmigrationandinvasionofovariancancercellbyinhibitingtusc2 AT huanwenting microrna663facilitatesthegrowthmigrationandinvasionofovariancancercellbyinhibitingtusc2 AT hanjiangqiong microrna663facilitatesthegrowthmigrationandinvasionofovariancancercellbyinhibitingtusc2 AT renweiru microrna663facilitatesthegrowthmigrationandinvasionofovariancancercellbyinhibitingtusc2 AT yanglihua microrna663facilitatesthegrowthmigrationandinvasionofovariancancercellbyinhibitingtusc2
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MicroRNA-663 facilitates the growth, migration and invasion of ovarian cancer cell by inhibiting TUSC2

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