Identification and characterization of CircRNAs involved in the regulation of wheat root length

Abstract Background: Recent studies indicate that circular RNAs (circRNAs) may play important roles in the regulation of plant growth and development. Plant roots are the main organs of nutrient and water uptake. However, whether circRNAs involved in the regulation of plant root growth remains to b...

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Autores principales:	Xu,Yanhua, Ren,Yongzhe, Lin,Tongbao, Cui,Dangqun
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	Triticum aestivum L. Root length CircRNAs Regulation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100217
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spelling	oai:scielo:S0716-976020190001002172019-10-10Identification and characterization of CircRNAs involved in the regulation of wheat root lengthXu,YanhuaRen,YongzheLin,TongbaoCui,Dangqun Triticum aestivum L. Root length CircRNAs Regulation Abstract Background: Recent studies indicate that circular RNAs (circRNAs) may play important roles in the regulation of plant growth and development. Plant roots are the main organs of nutrient and water uptake. However, whether circRNAs involved in the regulation of plant root growth remains to be elucidated. Methods: LH9, XN979 and YN29 are three Chinese wheat varieties with contrasting root lengths. Here, the root circRNA expression profiles of LH9, XN979 and YN29 were examined by using high-throughput sequencing technology. Results: Thirty-three and twenty-two differentially expressed circRNAs (DECs) were identified in the YN29-LH9 comparison and YN29-XN979 comparison, respectively. Among them, ten DECs coexisted in both comparisons. As the roots of both LH9 and XN979 were significantly larger and deeper than YN29, the ten DECs coexisting in the two comparisons were highly likely to be involved in the regulation of wheat root length. Moreover, three of the ten DECs have potential miRNA binding sites. Real-time PCR analysis showed that the expression levels of the potential binding miRNAs exhibited significant differences between the long root plants and the short root plants. Conclusions: The expression levels of some circRNAs exhibited significant differences in wheat varieties with contrasting root phenotypes. Ten DECs involved in the regulation of wheat root length were successfully identified in which three of them have potential miRNAs binding sites. The expression levels of putative circRNA-binding miRNAs were correlated with their corresponding circRNAs. Our results provide new clues for studying the potential roles of circRNAs in the regulation of wheat root length.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100217en10.1186/s40659-019-0228-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Triticum aestivum L. Root length CircRNAs Regulation
spellingShingle	Triticum aestivum L. Root length CircRNAs Regulation Xu,Yanhua Ren,Yongzhe Lin,Tongbao Cui,Dangqun Identification and characterization of CircRNAs involved in the regulation of wheat root length
description	Abstract Background: Recent studies indicate that circular RNAs (circRNAs) may play important roles in the regulation of plant growth and development. Plant roots are the main organs of nutrient and water uptake. However, whether circRNAs involved in the regulation of plant root growth remains to be elucidated. Methods: LH9, XN979 and YN29 are three Chinese wheat varieties with contrasting root lengths. Here, the root circRNA expression profiles of LH9, XN979 and YN29 were examined by using high-throughput sequencing technology. Results: Thirty-three and twenty-two differentially expressed circRNAs (DECs) were identified in the YN29-LH9 comparison and YN29-XN979 comparison, respectively. Among them, ten DECs coexisted in both comparisons. As the roots of both LH9 and XN979 were significantly larger and deeper than YN29, the ten DECs coexisting in the two comparisons were highly likely to be involved in the regulation of wheat root length. Moreover, three of the ten DECs have potential miRNA binding sites. Real-time PCR analysis showed that the expression levels of the potential binding miRNAs exhibited significant differences between the long root plants and the short root plants. Conclusions: The expression levels of some circRNAs exhibited significant differences in wheat varieties with contrasting root phenotypes. Ten DECs involved in the regulation of wheat root length were successfully identified in which three of them have potential miRNAs binding sites. The expression levels of putative circRNA-binding miRNAs were correlated with their corresponding circRNAs. Our results provide new clues for studying the potential roles of circRNAs in the regulation of wheat root length.
author	Xu,Yanhua Ren,Yongzhe Lin,Tongbao Cui,Dangqun
author_facet	Xu,Yanhua Ren,Yongzhe Lin,Tongbao Cui,Dangqun
author_sort	Xu,Yanhua
title	Identification and characterization of CircRNAs involved in the regulation of wheat root length
title_short	Identification and characterization of CircRNAs involved in the regulation of wheat root length
title_full	Identification and characterization of CircRNAs involved in the regulation of wheat root length
title_fullStr	Identification and characterization of CircRNAs involved in the regulation of wheat root length
title_full_unstemmed	Identification and characterization of CircRNAs involved in the regulation of wheat root length
title_sort	identification and characterization of circrnas involved in the regulation of wheat root length
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100217
work_keys_str_mv	AT xuyanhua identificationandcharacterizationofcircrnasinvolvedintheregulationofwheatrootlength AT renyongzhe identificationandcharacterizationofcircrnasinvolvedintheregulationofwheatrootlength AT lintongbao identificationandcharacterizationofcircrnasinvolvedintheregulationofwheatrootlength AT cuidangqun identificationandcharacterizationofcircrnasinvolvedintheregulationofwheatrootlength
_version_	1718441588164657152

Identification and characterization of CircRNAs involved in the regulation of wheat root length

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