Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adults
Abstract Background: Defects in DNA methylation have been shown to be associated with metabolic diseases such as obesity, dyslipidemia, and hypercholesterolemia. To analyze the methylation profile of the ADRB3 gene and correlate it with lipid profile, lipid intake, and oxidative stress based on mal...
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Sociedad de Biología de Chile
2019
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oai:scielo:S0716-976020190001002192019-10-10Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adultsLima,Raquel Patrícia AtaídeRibeiro,Marina RamalhoLima,Keylha Querino de FariasSena,Elisama Araújo deCosta,Diego de OliveiraLuna,Rafaella Cristhine PordeusNascimento,Rayner Anderson Ferreira doGonçalves,Maria da Conceição RodriguesVianna,Rodrigo Pinheiro de ToledoMoraes,Ronei Marcos deOliveira,Naila Francis Paulo deAlmeida,Aléssio Tony Cavalcanti deCosta,Maria José de Carvalho Epigenetics DNA methylation Obesity Lipid ADRB3 Abstract Background: Defects in DNA methylation have been shown to be associated with metabolic diseases such as obesity, dyslipidemia, and hypercholesterolemia. To analyze the methylation profile of the ADRB3 gene and correlate it with lipid profile, lipid intake, and oxidative stress based on malondialdehyde (MDA) and total antioxidant capacity (TAC), homocysteine and folic acid levels, nutritional status, lifestyle, and socioeconomic variables in an adult population. A cross-sectional epidemiological study representative of the East and West regions of the municipality of João Pessoa, Paraíba state, Brazil, enrolled 265 adults of both genders. Demographic, lifestyle, and socioeconomic questionnaires and a 24-h recall questionnaire were applied by trained interviewers' home. Nutritional and biochemical evaluation (DNA methylation, lipid profile, MDA, TAC, homocysteine and folic acid levels) was performed. Results: DNA hypermethylation of the ADRB3 gene, analyzed in leukocytes, was present in 50% of subjects and was associated with a higher risk of being overweight (OR 3.28; p = 0.008) or obese (OR 3.06; p = 0.017), a higher waist–hip ratio in males (OR 1.17; p = 0.000), greater intake of trans fats (OR 1.94; p = 0.032), higher LDL (OR 2.64; p = 0.003) and triglycerides (OR 1.81; p = 0.031), and higher folic acid levels (OR 1.85; p = 0.022). Conclusions: These results suggest that epigenetic changes in the ADRB3 gene locus may explain the development of obesity and non-communicable diseases associated with trans-fat intake, altered lipid profile, and elevated folic acid. Because of its persistence, DNA methylation may have an impact in adults, in association with the development of non-communicable diseases. This study is the first population-based study of the ADRB3 gene, and the data further support evaluation of ADRB3 DNA methylation as an effective biomarker.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100219en10.1186/s40659-019-0226-7 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Epigenetics DNA methylation Obesity Lipid ADRB3 |
| spellingShingle |
Epigenetics DNA methylation Obesity Lipid ADRB3 Lima,Raquel Patrícia Ataíde Ribeiro,Marina Ramalho Lima,Keylha Querino de Farias Sena,Elisama Araújo de Costa,Diego de Oliveira Luna,Rafaella Cristhine Pordeus Nascimento,Rayner Anderson Ferreira do Gonçalves,Maria da Conceição Rodrigues Vianna,Rodrigo Pinheiro de Toledo Moraes,Ronei Marcos de Oliveira,Naila Francis Paulo de Almeida,Aléssio Tony Cavalcanti de Costa,Maria José de Carvalho Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adults |
| description |
Abstract Background: Defects in DNA methylation have been shown to be associated with metabolic diseases such as obesity, dyslipidemia, and hypercholesterolemia. To analyze the methylation profile of the ADRB3 gene and correlate it with lipid profile, lipid intake, and oxidative stress based on malondialdehyde (MDA) and total antioxidant capacity (TAC), homocysteine and folic acid levels, nutritional status, lifestyle, and socioeconomic variables in an adult population. A cross-sectional epidemiological study representative of the East and West regions of the municipality of João Pessoa, Paraíba state, Brazil, enrolled 265 adults of both genders. Demographic, lifestyle, and socioeconomic questionnaires and a 24-h recall questionnaire were applied by trained interviewers' home. Nutritional and biochemical evaluation (DNA methylation, lipid profile, MDA, TAC, homocysteine and folic acid levels) was performed. Results: DNA hypermethylation of the ADRB3 gene, analyzed in leukocytes, was present in 50% of subjects and was associated with a higher risk of being overweight (OR 3.28; p = 0.008) or obese (OR 3.06; p = 0.017), a higher waist–hip ratio in males (OR 1.17; p = 0.000), greater intake of trans fats (OR 1.94; p = 0.032), higher LDL (OR 2.64; p = 0.003) and triglycerides (OR 1.81; p = 0.031), and higher folic acid levels (OR 1.85; p = 0.022). Conclusions: These results suggest that epigenetic changes in the ADRB3 gene locus may explain the development of obesity and non-communicable diseases associated with trans-fat intake, altered lipid profile, and elevated folic acid. Because of its persistence, DNA methylation may have an impact in adults, in association with the development of non-communicable diseases. This study is the first population-based study of the ADRB3 gene, and the data further support evaluation of ADRB3 DNA methylation as an effective biomarker. |
| author |
Lima,Raquel Patrícia Ataíde Ribeiro,Marina Ramalho Lima,Keylha Querino de Farias Sena,Elisama Araújo de Costa,Diego de Oliveira Luna,Rafaella Cristhine Pordeus Nascimento,Rayner Anderson Ferreira do Gonçalves,Maria da Conceição Rodrigues Vianna,Rodrigo Pinheiro de Toledo Moraes,Ronei Marcos de Oliveira,Naila Francis Paulo de Almeida,Aléssio Tony Cavalcanti de Costa,Maria José de Carvalho |
| author_facet |
Lima,Raquel Patrícia Ataíde Ribeiro,Marina Ramalho Lima,Keylha Querino de Farias Sena,Elisama Araújo de Costa,Diego de Oliveira Luna,Rafaella Cristhine Pordeus Nascimento,Rayner Anderson Ferreira do Gonçalves,Maria da Conceição Rodrigues Vianna,Rodrigo Pinheiro de Toledo Moraes,Ronei Marcos de Oliveira,Naila Francis Paulo de Almeida,Aléssio Tony Cavalcanti de Costa,Maria José de Carvalho |
| author_sort |
Lima,Raquel Patrícia Ataíde |
| title |
Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adults |
| title_short |
Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adults |
| title_full |
Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adults |
| title_fullStr |
Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adults |
| title_full_unstemmed |
Methylation profile of the ADRB3 gene and its association with lipid profile and nutritional status in adults |
| title_sort |
methylation profile of the adrb3 gene and its association with lipid profile and nutritional status in adults |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2019 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100219 |
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