Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts

Abstract Background: Conjunctival filtering bleb scar formation is the main reason for the failure of glaucoma filtration surgery. Cytoglobin (Cygb) has been reported to play an important role in extracellular matrix (ECM) remodeling, fibrosis and tissue damage repairing. This study aimed to invest...

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Autores principales: Wei,Haiying, Lin,Lili, Zhang,Xiaomei, Feng,Zhuolei, Wang,Yeqing, You,Yan, Wang,Xiaodan, Hou,Yongsheng
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2019
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100221
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spelling oai:scielo:S0716-976020190001002212019-10-10Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblastsWei,HaiyingLin,LiliZhang,XiaomeiFeng,ZhuoleiWang,YeqingYou,YanWang,XiaodanHou,Yongsheng Glaucoma Cytoglobin Human tenon fibroblast Extracellular matrix Abstract Background: Conjunctival filtering bleb scar formation is the main reason for the failure of glaucoma filtration surgery. Cytoglobin (Cygb) has been reported to play an important role in extracellular matrix (ECM) remodeling, fibrosis and tissue damage repairing. This study aimed to investigate the role of Cygb in anti-scarring during excessive conjunctival wound healing after glaucoma filtration surgery. Methods: Cygb was overexpressed in human tenon fibroblasts (hTFs) by transfecting hTFs with lentiviral particles encoding pLenti6.2-FLAG-Cygb. Changes in the mRNA and protein levels of fibronectin, collagen I, collagen III, TGF-&#946;1, and HIF1&#945; were determined by RT-PCR and western blotting respectively. Results: After Cygb overexpression, hTFs displayed no significant changes in visual appearance and cell counts compared to controls. Whereas, Cygb overexpression significantly decreased the mRNA and protein expression levels of collagen I, collagen III and fibronectin compared with control (p < 0.01). There was also a statistically significant decrease in the mRNA and protein levels of TGF-&#946;1 and HIF-1&#945; in hTFs with overexpressed Cygb compared with control group (p < 0.05). Conclusion: Our study provided evidence that overexpression of Cygb decreased the expression levels of fibronectin, collagen I, collagen III, TGF-&#946;1 and HIF-1&#945; in hTFs. Therefore, therapies targeting Cygb expression in hTFs may pave a new way for clinicians to solve the problem of post-glaucoma surgery scarring.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100221en10.1186/s40659-019-0229-4
institution Scielo Chile
collection Scielo Chile
language English
topic Glaucoma
Cytoglobin
Human tenon fibroblast
Extracellular matrix
spellingShingle Glaucoma
Cytoglobin
Human tenon fibroblast
Extracellular matrix
Wei,Haiying
Lin,Lili
Zhang,Xiaomei
Feng,Zhuolei
Wang,Yeqing
You,Yan
Wang,Xiaodan
Hou,Yongsheng
Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts
description Abstract Background: Conjunctival filtering bleb scar formation is the main reason for the failure of glaucoma filtration surgery. Cytoglobin (Cygb) has been reported to play an important role in extracellular matrix (ECM) remodeling, fibrosis and tissue damage repairing. This study aimed to investigate the role of Cygb in anti-scarring during excessive conjunctival wound healing after glaucoma filtration surgery. Methods: Cygb was overexpressed in human tenon fibroblasts (hTFs) by transfecting hTFs with lentiviral particles encoding pLenti6.2-FLAG-Cygb. Changes in the mRNA and protein levels of fibronectin, collagen I, collagen III, TGF-&#946;1, and HIF1&#945; were determined by RT-PCR and western blotting respectively. Results: After Cygb overexpression, hTFs displayed no significant changes in visual appearance and cell counts compared to controls. Whereas, Cygb overexpression significantly decreased the mRNA and protein expression levels of collagen I, collagen III and fibronectin compared with control (p < 0.01). There was also a statistically significant decrease in the mRNA and protein levels of TGF-&#946;1 and HIF-1&#945; in hTFs with overexpressed Cygb compared with control group (p < 0.05). Conclusion: Our study provided evidence that overexpression of Cygb decreased the expression levels of fibronectin, collagen I, collagen III, TGF-&#946;1 and HIF-1&#945; in hTFs. Therefore, therapies targeting Cygb expression in hTFs may pave a new way for clinicians to solve the problem of post-glaucoma surgery scarring.
author Wei,Haiying
Lin,Lili
Zhang,Xiaomei
Feng,Zhuolei
Wang,Yeqing
You,Yan
Wang,Xiaodan
Hou,Yongsheng
author_facet Wei,Haiying
Lin,Lili
Zhang,Xiaomei
Feng,Zhuolei
Wang,Yeqing
You,Yan
Wang,Xiaodan
Hou,Yongsheng
author_sort Wei,Haiying
title Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts
title_short Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts
title_full Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts
title_fullStr Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts
title_full_unstemmed Effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts
title_sort effect of cytoglobin overexpression on extracellular matrix component synthesis in human tenon fibroblasts
publisher Sociedad de Biología de Chile
publishDate 2019
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100221
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AT fengzhuolei effectofcytoglobinoverexpressiononextracellularmatrixcomponentsynthesisinhumantenonfibroblasts
AT wangyeqing effectofcytoglobinoverexpressiononextracellularmatrixcomponentsynthesisinhumantenonfibroblasts
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AT houyongsheng effectofcytoglobinoverexpressiononextracellularmatrixcomponentsynthesisinhumantenonfibroblasts
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