PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer

Abstract Background: To analyze the relative expression of PELI3 and its mechanistic involvement in the non-small cell lung cancer (NSCLC). Methods: PELI3 expression in NSCLC tissue samples was determined by the immunohistochemistry. The transcripts abundance of PELI3 was measured with real-time P...

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Autores principales:	He,Yuzheng, Shi,Yantao, Liu,Ruilin, Wang,Zhichao, Wang,Baohua, Li,Shujun, Zhang,Helin
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	PELI3 miR-365a-5p Non-small cell lung cancer Cell viability
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100222
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spelling	oai:scielo:S0716-976020190001002222019-10-10PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancerHe,YuzhengShi,YantaoLiu,RuilinWang,ZhichaoWang,BaohuaLi,ShujunZhang,Helin PELI3 miR-365a-5p Non-small cell lung cancer Cell viability Abstract Background: To analyze the relative expression of PELI3 and its mechanistic involvement in the non-small cell lung cancer (NSCLC). Methods: PELI3 expression in NSCLC tissue samples was determined by the immunohistochemistry. The transcripts abundance of PELI3 was measured with real-time PCR. The protein intensity was analyzed by western blot. The overall survival in respect to PELI3 or miR-365a-5p expression was plotted by the Kaplan–Meier's analysis. Cell growth was determined by colony formation assay. Cell viability was measured by MTT assay. The migration and invasion were evaluated by wound healing and transwell assay respectively. The regulatory effect of miR-365a-5p on PELI3 was interrogated with luciferase reporter assay. The direct binding between miR-365a-5p and PELI3 was analyzed by pulldown assay. Results: PELI3 was aberrantly up-regulated in NSCLC both in vivo and in vitro. High level of PELI3 associated with poor prognosis. PELI3-deficiency significantly inhibited cell viability, colony formation, migration and invasion. We further identified that miR-365a-5p negatively regulated PELI3 in this disease. Ectopic expression of miR-365a-5p in both A549 and H1299 phenocopied PELI3-deficiency. Meanwhile, PELI3-silencing significantly abolished the pro-tumoral effect elicited by miR-365a-5p inhibition. Conclusion: Our results highlighted the importance of dysregulated miR-365a-5p-PELI3 signaling axis in NSCLC.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100222en10.1186/s40659-019-0230-y
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	PELI3 miR-365a-5p Non-small cell lung cancer Cell viability
spellingShingle	PELI3 miR-365a-5p Non-small cell lung cancer Cell viability He,Yuzheng Shi,Yantao Liu,Ruilin Wang,Zhichao Wang,Baohua Li,Shujun Zhang,Helin PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer
description	Abstract Background: To analyze the relative expression of PELI3 and its mechanistic involvement in the non-small cell lung cancer (NSCLC). Methods: PELI3 expression in NSCLC tissue samples was determined by the immunohistochemistry. The transcripts abundance of PELI3 was measured with real-time PCR. The protein intensity was analyzed by western blot. The overall survival in respect to PELI3 or miR-365a-5p expression was plotted by the Kaplan–Meier's analysis. Cell growth was determined by colony formation assay. Cell viability was measured by MTT assay. The migration and invasion were evaluated by wound healing and transwell assay respectively. The regulatory effect of miR-365a-5p on PELI3 was interrogated with luciferase reporter assay. The direct binding between miR-365a-5p and PELI3 was analyzed by pulldown assay. Results: PELI3 was aberrantly up-regulated in NSCLC both in vivo and in vitro. High level of PELI3 associated with poor prognosis. PELI3-deficiency significantly inhibited cell viability, colony formation, migration and invasion. We further identified that miR-365a-5p negatively regulated PELI3 in this disease. Ectopic expression of miR-365a-5p in both A549 and H1299 phenocopied PELI3-deficiency. Meanwhile, PELI3-silencing significantly abolished the pro-tumoral effect elicited by miR-365a-5p inhibition. Conclusion: Our results highlighted the importance of dysregulated miR-365a-5p-PELI3 signaling axis in NSCLC.
author	He,Yuzheng Shi,Yantao Liu,Ruilin Wang,Zhichao Wang,Baohua Li,Shujun Zhang,Helin
author_facet	He,Yuzheng Shi,Yantao Liu,Ruilin Wang,Zhichao Wang,Baohua Li,Shujun Zhang,Helin
author_sort	He,Yuzheng
title	PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer
title_short	PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer
title_full	PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer
title_fullStr	PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer
title_full_unstemmed	PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer
title_sort	peli3 mediates pro-tumor actions of down-regulated mir-365a-5p in non-small cell lung cancer
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100222
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PELI3 mediates pro-tumor actions of down-regulated miR-365a-5p in non-small cell lung cancer

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