Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic‑ischemic encephalopathy

Abstract Background: Hypoxic-ischemic encephalopathy (HIE) is a common disease that occurs during the perinatal period. The primary cause of neonatal HIE is related to fetal intrauterine anoxia. Carbamylated erythropoietin (CEPO), a derivative of erythropoietin (EPO), does not exert any erythropoie...

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Autores principales:	Diao,Min, Qu,Yi, Liu,Hui, Ma,Yushan, Lin,Xuemei
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	Carbamylated erythropoietin Neuronal apoptosis Intrauterine hypoxic-ischemic encephalopathy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100226
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spelling	oai:scielo:S0716-976020190001002262019-10-10Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic‑ischemic encephalopathyDiao,MinQu,YiLiu,HuiMa,YushanLin,Xuemei Carbamylated erythropoietin Neuronal apoptosis Intrauterine hypoxic-ischemic encephalopathy Abstract Background: Hypoxic-ischemic encephalopathy (HIE) is a common disease that occurs during the perinatal period. The primary cause of neonatal HIE is related to fetal intrauterine anoxia. Carbamylated erythropoietin (CEPO), a derivative of erythropoietin (EPO), does not exert any erythropoietic effect; however, the neuroprotective effects resemble those of EPO. Previous studies have shown the potential benefits of CEPO on the central nervous system. The present study aimed to investigate the role of CEPO in neuronal apoptosis during intrauterine HIE and the underlying mechanisms. Results: To validate our hypothesis, we established an intrauterine HIE model by occluding the bilateral uteroovarian arteries of pregnant Sprague–Dawley rats. Compared to the I/R group, neuronal apoptosis in the CEPO group was significantly lower at 4, 12, 24, and 48 h (P < 0.05). CEPO significantly inhibited CC3 expression (P < 0.05) during the early-stages after ischemia–reperfusion (0.5, 4, 8, 12 and 24 h), upregulated Bcl-2 expression, and downregulated Bax expression at 4, 8, 12, and 24 h (P < 0.05). Conclusions: Carbamylated erythropoietin pretreatment inhibited the expression of proapoptotic protein CC3 in brain and regulated the Bcl-2/Bax ratio, resulting in reduced neuronal apoptosis and thus resulting in a protective effect on intrauterine HIE.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100226en10.1186/s40659-019-0234-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Carbamylated erythropoietin Neuronal apoptosis Intrauterine hypoxic-ischemic encephalopathy
spellingShingle	Carbamylated erythropoietin Neuronal apoptosis Intrauterine hypoxic-ischemic encephalopathy Diao,Min Qu,Yi Liu,Hui Ma,Yushan Lin,Xuemei Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic‑ischemic encephalopathy
description	Abstract Background: Hypoxic-ischemic encephalopathy (HIE) is a common disease that occurs during the perinatal period. The primary cause of neonatal HIE is related to fetal intrauterine anoxia. Carbamylated erythropoietin (CEPO), a derivative of erythropoietin (EPO), does not exert any erythropoietic effect; however, the neuroprotective effects resemble those of EPO. Previous studies have shown the potential benefits of CEPO on the central nervous system. The present study aimed to investigate the role of CEPO in neuronal apoptosis during intrauterine HIE and the underlying mechanisms. Results: To validate our hypothesis, we established an intrauterine HIE model by occluding the bilateral uteroovarian arteries of pregnant Sprague–Dawley rats. Compared to the I/R group, neuronal apoptosis in the CEPO group was significantly lower at 4, 12, 24, and 48 h (P < 0.05). CEPO significantly inhibited CC3 expression (P < 0.05) during the early-stages after ischemia–reperfusion (0.5, 4, 8, 12 and 24 h), upregulated Bcl-2 expression, and downregulated Bax expression at 4, 8, 12, and 24 h (P < 0.05). Conclusions: Carbamylated erythropoietin pretreatment inhibited the expression of proapoptotic protein CC3 in brain and regulated the Bcl-2/Bax ratio, resulting in reduced neuronal apoptosis and thus resulting in a protective effect on intrauterine HIE.
author	Diao,Min Qu,Yi Liu,Hui Ma,Yushan Lin,Xuemei
author_facet	Diao,Min Qu,Yi Liu,Hui Ma,Yushan Lin,Xuemei
author_sort	Diao,Min
title	Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic‑ischemic encephalopathy
title_short	Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic‑ischemic encephalopathy
title_full	Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic‑ischemic encephalopathy
title_fullStr	Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic‑ischemic encephalopathy
title_full_unstemmed	Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic‑ischemic encephalopathy
title_sort	effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic‑ischemic encephalopathy
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100226
work_keys_str_mv	AT diaomin effectofcarbamylatederythropoietinonneuronalapoptosisinfetalratsduringintrauterinehypoxic8209ischemicencephalopathy AT quyi effectofcarbamylatederythropoietinonneuronalapoptosisinfetalratsduringintrauterinehypoxic8209ischemicencephalopathy AT liuhui effectofcarbamylatederythropoietinonneuronalapoptosisinfetalratsduringintrauterinehypoxic8209ischemicencephalopathy AT mayushan effectofcarbamylatederythropoietinonneuronalapoptosisinfetalratsduringintrauterinehypoxic8209ischemicencephalopathy AT linxuemei effectofcarbamylatederythropoietinonneuronalapoptosisinfetalratsduringintrauterinehypoxic8209ischemicencephalopathy
_version_	1718441590327869440

Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic&#8209;ischemic encephalopathy

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Effect of carbamylated erythropoietin on neuronal apoptosis in fetal rats during intrauterine hypoxic‑ischemic encephalopathy