Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 mice

Abstract Background: Chronic prostatitis has been supposed to be associated with preneoplastic lesions and cancer development. The objective of this study was to examine how chronic inflammation results in a prostatic microenvironment and gene mutation in C57BL/6 mice. Methods: Immune and bacteria...

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Autores principales:	Gao,Yong, Wei,Lijuan, Wang,Chenbang, Huang,Yuanjie, Li,Weidong, Li,Tianyu, Mo,Chaohua, Qin,Huali, Zhong,Xiaoge, Wang,Yun, Tan,Aihua, Mo,Zengnan, Jiang,Yonghua, Hu,Yanling
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	Chronic prostatitis Prostatitis mouse model Proliferative inflammatory atrophy Prostatic intraepithelial neoplasia Somatic genome mutation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100228
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spelling	oai:scielo:S0716-976020190001002282019-10-10Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 miceGao,YongWei,LijuanWang,ChenbangHuang,YuanjieLi,WeidongLi,TianyuMo,ChaohuaQin,HualiZhong,XiaogeWang,YunTan,AihuaMo,ZengnanJiang,YonghuaHu,Yanling Chronic prostatitis Prostatitis mouse model Proliferative inflammatory atrophy Prostatic intraepithelial neoplasia Somatic genome mutation Abstract Background: Chronic prostatitis has been supposed to be associated with preneoplastic lesions and cancer development. The objective of this study was to examine how chronic inflammation results in a prostatic microenvironment and gene mutation in C57BL/6 mice. Methods: Immune and bacterial prostatitis mouse models were created through abdominal subcutaneous injection of rat prostate extract protein immunization (EAP group) or transurethral instillation of uropathogenic E. coli 1677 (E. coli group). Prostate histology, serum cytokine level, and genome-wide exome (GWE) sequences were examined 1, 3, and 6 months after immunization or injection. Result: In the EAP and E. coli groups, immune cell infiltrations were observed in the first and last months of the entire experiment. After 3 months, obvious proliferative inflammatory atrophy (PIA) and prostatic intraepithelial neoplasia (PIN) were observed accompanied with fibrosis hyperplasia in stroma. The decrease in basal cells (Cytokeratin (CK) 5+/p63+) and the accumulation of luminal epithelial cells (CK8+) in the PIA or PIN area indicated that the basal cells were damaged or transformed into different luminal cells. Hic1, Zfp148, and Mfge8 gene mutations were detected in chronic prostatitis somatic cells. Conclusion: Chronic prostatitis induced by prostate extract protein immunization or E. coli infection caused a reactive prostatic inflammation microenvironment and resulted in tissue damage, aberrant atrophy, hyperplasia, and somatic genome mutation.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100228en10.1186/s40659-019-0237-4
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Chronic prostatitis Prostatitis mouse model Proliferative inflammatory atrophy Prostatic intraepithelial neoplasia Somatic genome mutation
spellingShingle	Chronic prostatitis Prostatitis mouse model Proliferative inflammatory atrophy Prostatic intraepithelial neoplasia Somatic genome mutation Gao,Yong Wei,Lijuan Wang,Chenbang Huang,Yuanjie Li,Weidong Li,Tianyu Mo,Chaohua Qin,Huali Zhong,Xiaoge Wang,Yun Tan,Aihua Mo,Zengnan Jiang,Yonghua Hu,Yanling Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 mice
description	Abstract Background: Chronic prostatitis has been supposed to be associated with preneoplastic lesions and cancer development. The objective of this study was to examine how chronic inflammation results in a prostatic microenvironment and gene mutation in C57BL/6 mice. Methods: Immune and bacterial prostatitis mouse models were created through abdominal subcutaneous injection of rat prostate extract protein immunization (EAP group) or transurethral instillation of uropathogenic E. coli 1677 (E. coli group). Prostate histology, serum cytokine level, and genome-wide exome (GWE) sequences were examined 1, 3, and 6 months after immunization or injection. Result: In the EAP and E. coli groups, immune cell infiltrations were observed in the first and last months of the entire experiment. After 3 months, obvious proliferative inflammatory atrophy (PIA) and prostatic intraepithelial neoplasia (PIN) were observed accompanied with fibrosis hyperplasia in stroma. The decrease in basal cells (Cytokeratin (CK) 5+/p63+) and the accumulation of luminal epithelial cells (CK8+) in the PIA or PIN area indicated that the basal cells were damaged or transformed into different luminal cells. Hic1, Zfp148, and Mfge8 gene mutations were detected in chronic prostatitis somatic cells. Conclusion: Chronic prostatitis induced by prostate extract protein immunization or E. coli infection caused a reactive prostatic inflammation microenvironment and resulted in tissue damage, aberrant atrophy, hyperplasia, and somatic genome mutation.
author	Gao,Yong Wei,Lijuan Wang,Chenbang Huang,Yuanjie Li,Weidong Li,Tianyu Mo,Chaohua Qin,Huali Zhong,Xiaoge Wang,Yun Tan,Aihua Mo,Zengnan Jiang,Yonghua Hu,Yanling
author_facet	Gao,Yong Wei,Lijuan Wang,Chenbang Huang,Yuanjie Li,Weidong Li,Tianyu Mo,Chaohua Qin,Huali Zhong,Xiaoge Wang,Yun Tan,Aihua Mo,Zengnan Jiang,Yonghua Hu,Yanling
author_sort	Gao,Yong
title	Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 mice
title_short	Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 mice
title_full	Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 mice
title_fullStr	Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 mice
title_full_unstemmed	Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 mice
title_sort	chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in c57bl/6 mice
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100228
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Chronic prostatitis alters the prostatic microenvironment and accelerates preneoplastic lesions in C57BL/6 mice

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