Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma

Abstract Background: The purpose of the present study was to investigate the role of the methylation status of the DACT1 gene on the invasion and metastasis of nasopharyngeal carcinoma cells. Methods: The levels of methylation and expression of the DACT1 gene in nasopharyngeal carcinoma tissues an...

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Autores principales:	Yang,Ju-Hong, Lin,Lie-Kun, Zhang,Song
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	DACT1 gene Nasopharyngeal carcinoma CNE2 cell line Invasion Metastasis 5-Aza-2-deoxycytidine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100229
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spelling	oai:scielo:S0716-976020190001002292019-10-10Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinomaYang,Ju-HongLin,Lie-KunZhang,Song DACT1 gene Nasopharyngeal carcinoma CNE2 cell line Invasion Metastasis 5-Aza-2-deoxycytidine Abstract Background: The purpose of the present study was to investigate the role of the methylation status of the DACT1 gene on the invasion and metastasis of nasopharyngeal carcinoma cells. Methods: The levels of methylation and expression of the DACT1 gene in nasopharyngeal carcinoma tissues and CNE2 cells were determined by methylation–specific PCR and RT–PCR, respectively. CNE2 cells were treated with 5–aza–2–deoxycytidine, and the variation in the methylation status of the DACT1 gene was detected, as well as the influence of methylation on invasiveness of nasopharyngeal carcinoma cells. Results: The DACT1 gene was hyper–methylated in 44 of 62 cases of nasopharyngeal carcinoma. The DACT1 gene was hyper–methylated in 32 of 38 cases of nasopharyngeal carcinoma with lymph node metastasis, and the DACT1 gene was hyper–methylated in 7 of 24 cases of nasopharyngeal carcinoma without lymph node metastasis. The DACT1 mRNA level was weakly expressed or not expressed in all nasopharyngeal carcinoma tissues with hyper–methylated DACT1 genes; however, the DACT1 mRNA level was highly expressed in nasopharyngeal carcinoma tissues with low expression of the methylated DACT1 gene. The DACT1 gene was hyper–methylated and not expressed in CNE2 cells that did not have 5–aza–2–deoxycytidine treatment. After 5–aza–2–deoxycytidine treatment, the DACT1 gene was demethylated and the expression of DACT1 was restored. Moreover, the invasion ability was inhibited in CNE2 cells treated with 5–aza–2–deoxycytidine. Conclusion: The expression of DACT1 was related to the methylation status. High expression of DACT1 may inhibit the invasion and metastasis of nasopharyngeal carcinoma cells.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100229en10.1186/s40659-019-0238-3
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DACT1 gene Nasopharyngeal carcinoma CNE2 cell line Invasion Metastasis 5-Aza-2-deoxycytidine
spellingShingle	DACT1 gene Nasopharyngeal carcinoma CNE2 cell line Invasion Metastasis 5-Aza-2-deoxycytidine Yang,Ju-Hong Lin,Lie-Kun Zhang,Song Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma
description	Abstract Background: The purpose of the present study was to investigate the role of the methylation status of the DACT1 gene on the invasion and metastasis of nasopharyngeal carcinoma cells. Methods: The levels of methylation and expression of the DACT1 gene in nasopharyngeal carcinoma tissues and CNE2 cells were determined by methylation–specific PCR and RT–PCR, respectively. CNE2 cells were treated with 5–aza–2–deoxycytidine, and the variation in the methylation status of the DACT1 gene was detected, as well as the influence of methylation on invasiveness of nasopharyngeal carcinoma cells. Results: The DACT1 gene was hyper–methylated in 44 of 62 cases of nasopharyngeal carcinoma. The DACT1 gene was hyper–methylated in 32 of 38 cases of nasopharyngeal carcinoma with lymph node metastasis, and the DACT1 gene was hyper–methylated in 7 of 24 cases of nasopharyngeal carcinoma without lymph node metastasis. The DACT1 mRNA level was weakly expressed or not expressed in all nasopharyngeal carcinoma tissues with hyper–methylated DACT1 genes; however, the DACT1 mRNA level was highly expressed in nasopharyngeal carcinoma tissues with low expression of the methylated DACT1 gene. The DACT1 gene was hyper–methylated and not expressed in CNE2 cells that did not have 5–aza–2–deoxycytidine treatment. After 5–aza–2–deoxycytidine treatment, the DACT1 gene was demethylated and the expression of DACT1 was restored. Moreover, the invasion ability was inhibited in CNE2 cells treated with 5–aza–2–deoxycytidine. Conclusion: The expression of DACT1 was related to the methylation status. High expression of DACT1 may inhibit the invasion and metastasis of nasopharyngeal carcinoma cells.
author	Yang,Ju-Hong Lin,Lie-Kun Zhang,Song
author_facet	Yang,Ju-Hong Lin,Lie-Kun Zhang,Song
author_sort	Yang,Ju-Hong
title	Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma
title_short	Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma
title_full	Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma
title_fullStr	Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma
title_full_unstemmed	Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma
title_sort	effects of dact1 methylation status on invasion and metastasis of nasopharyngeal carcinoma
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100229
work_keys_str_mv	AT yangjuhong effectsofdact1methylationstatusoninvasionandmetastasisofnasopharyngealcarcinoma AT linliekun effectsofdact1methylationstatusoninvasionandmetastasisofnasopharyngealcarcinoma AT zhangsong effectsofdact1methylationstatusoninvasionandmetastasisofnasopharyngealcarcinoma
_version_	1718441591108009984

Effects of DACT1 methylation status on invasion and metastasis of nasopharyngeal carcinoma

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