LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway

Abstract Background: Studies have shown that cancer susceptibility candidate 11 (CASC11), a newly discovered long non-coding RNA (lncRNA), was aberrantly overexpressed in hepatic carcinoma, gastric cancer and colorectal cancer. However, its effects on cervical cancer has been kept unknown up to now...

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Autores principales:	Hsu,Wenchan, Liu,Lifen, Chen,Xin, Zhang,Ying, Zhu,Weipei
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	LncRNA CASC11 Cervical cancer Wnt/β-catenin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100231
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spelling	oai:scielo:S0716-976020190001002312019-10-10LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathwayHsu,WenchanLiu,LifenChen,XinZhang,YingZhu,Weipei LncRNA CASC11 Cervical cancer Wnt/β-catenin Abstract Background: Studies have shown that cancer susceptibility candidate 11 (CASC11), a newly discovered long non-coding RNA (lncRNA), was aberrantly overexpressed in hepatic carcinoma, gastric cancer and colorectal cancer. However, its effects on cervical cancer has been kept unknown up to now. The present study was aimed to investigate the relationship between lncRNA CASC11 and cervical cancer and further explore the mechanism of CASC11 effect on cervical cancer progression. Materials: Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the expressions of CASC11 in cancerous and adjacent normal tissues of patients with cervical cancer as well as in cell lines. The proliferation, migration, invasion and apoptosis were assayed after transfecting the cell with si-CASC11 or pcDNA3.1-CASC11. TOP/FOP-Flash luciferase reporter assay and western blot were used to analysis the activation of Wnt/β-catenin signaling pathway. Si-CASC11-transfected HeLa cells were subcutaneously inoculated into male athymic (nude) mice to investigate the effect of CASC11 on the tumor formation. Results: We discovered that CASC11, the expression of which was positively associated with the tumor size and the FIGO staging and negatively related to the patients' survival rate, was up-regulated in the cervical cancer tissues and cell lines. Silencing CASC11 inhibited the proliferation, migration as well as invasion and promoted the cell apoptosis. Conversely, overexpression of CASC11 facilitated the cancer cell's proliferation, migration and invasion ability and suppressed the apoptosis. Further study showed that CASC11 promoted the migration and invasion of cervical cancer cells by activating Wnt/β-catenin signaling pathway and silencing CASC11 inhibited the tumor growth in vivo. Conclusion: Our study demonstrated that CASC11 promoted the cervical cancer progression by activating Wnt/β-catenin signaling pathway for the first time, which provides a new target or a potential diagnostic biomarker of the treatment for cervical cancer.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100231en10.1186/s40659-019-0240-9
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	LncRNA CASC11 Cervical cancer Wnt/β-catenin
spellingShingle	LncRNA CASC11 Cervical cancer Wnt/β-catenin Hsu,Wenchan Liu,Lifen Chen,Xin Zhang,Ying Zhu,Weipei LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway
description	Abstract Background: Studies have shown that cancer susceptibility candidate 11 (CASC11), a newly discovered long non-coding RNA (lncRNA), was aberrantly overexpressed in hepatic carcinoma, gastric cancer and colorectal cancer. However, its effects on cervical cancer has been kept unknown up to now. The present study was aimed to investigate the relationship between lncRNA CASC11 and cervical cancer and further explore the mechanism of CASC11 effect on cervical cancer progression. Materials: Quantitative real-time polymerase chain reaction (RT-qPCR) was used to detect the expressions of CASC11 in cancerous and adjacent normal tissues of patients with cervical cancer as well as in cell lines. The proliferation, migration, invasion and apoptosis were assayed after transfecting the cell with si-CASC11 or pcDNA3.1-CASC11. TOP/FOP-Flash luciferase reporter assay and western blot were used to analysis the activation of Wnt/β-catenin signaling pathway. Si-CASC11-transfected HeLa cells were subcutaneously inoculated into male athymic (nude) mice to investigate the effect of CASC11 on the tumor formation. Results: We discovered that CASC11, the expression of which was positively associated with the tumor size and the FIGO staging and negatively related to the patients' survival rate, was up-regulated in the cervical cancer tissues and cell lines. Silencing CASC11 inhibited the proliferation, migration as well as invasion and promoted the cell apoptosis. Conversely, overexpression of CASC11 facilitated the cancer cell's proliferation, migration and invasion ability and suppressed the apoptosis. Further study showed that CASC11 promoted the migration and invasion of cervical cancer cells by activating Wnt/β-catenin signaling pathway and silencing CASC11 inhibited the tumor growth in vivo. Conclusion: Our study demonstrated that CASC11 promoted the cervical cancer progression by activating Wnt/β-catenin signaling pathway for the first time, which provides a new target or a potential diagnostic biomarker of the treatment for cervical cancer.
author	Hsu,Wenchan Liu,Lifen Chen,Xin Zhang,Ying Zhu,Weipei
author_facet	Hsu,Wenchan Liu,Lifen Chen,Xin Zhang,Ying Zhu,Weipei
author_sort	Hsu,Wenchan
title	LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway
title_short	LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway
title_full	LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway
title_fullStr	LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway
title_full_unstemmed	LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway
title_sort	lncrna casc11 promotes the cervical cancer progression by activating wnt/beta-catenin signaling pathway
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100231
work_keys_str_mv	AT hsuwenchan lncrnacasc11promotesthecervicalcancerprogressionbyactivatingwntbetacateninsignalingpathway AT liulifen lncrnacasc11promotesthecervicalcancerprogressionbyactivatingwntbetacateninsignalingpathway AT chenxin lncrnacasc11promotesthecervicalcancerprogressionbyactivatingwntbetacateninsignalingpathway AT zhangying lncrnacasc11promotesthecervicalcancerprogressionbyactivatingwntbetacateninsignalingpathway AT zhuweipei lncrnacasc11promotesthecervicalcancerprogressionbyactivatingwntbetacateninsignalingpathway
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LncRNA CASC11 promotes the cervical cancer progression by activating Wnt/beta-catenin signaling pathway

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