Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells

Abstract Background: Psoralen is a coumarin-like and coumarin-related benzofuran glycoside, which is a commonly used traditional Chinese medicine to treat patients with kidney and spleen-yang deficiency symptom. Psoralen has been reported to show estrogen-like activity, antioxidant activity, osteob...

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Autores principales:	Wang,Xiaomin, Peng,Peike, Pan,Zhiqiang, Fang,Zhaoqin, Lu,Wenli, Liu,Xiaomei
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	Psoralen Hepatocellular carcinoma SMMC7721 cell Endoplasmic reticulum stress Apoptosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100232
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spelling	oai:scielo:S0716-976020190001002322019-10-10Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cellsWang,XiaominPeng,PeikePan,ZhiqiangFang,ZhaoqinLu,WenliLiu,Xiaomei Psoralen Hepatocellular carcinoma SMMC7721 cell Endoplasmic reticulum stress Apoptosis Abstract Background: Psoralen is a coumarin-like and coumarin-related benzofuran glycoside, which is a commonly used traditional Chinese medicine to treat patients with kidney and spleen-yang deficiency symptom. Psoralen has been reported to show estrogen-like activity, antioxidant activity, osteoblastic proliferation accelerating activity, antitumor effects and antibacterial activity. However, the antitumor mechanism of psoralen is not fully understood. This study aimed to investigate the therapeutic efficacy of psoralen in human hepatoma cell line SMMC7721 and the mechanism of antitumor effects. Results: Psoralen inhibited proliferation of SMMC7721 in a dose- and time-dependent manner, and promoted apoptosis. Further, psoralen activated the ER stress signal pathway, including the expansion of endoplasmic reticulum, increasing the mRNA levels of GRP78, DDIT3, ATF4, XBP1, GADD34 and the protein levels of GDF15, GRP78, IRE1α, XBP-1s in a time-dependent manner. Psoralen induces cell cycle arrest at G1 phase by enhancing CyclinD1 and reducing CyclinE1 expression. Moreover, TUDC couldn't inhibit the psoralen-induced ER stress in SMMC7721 cells. Conclusions: Psoralen can inhibit the proliferation of SMMC7721 cells and induce ER stress response to induce cell apoptosis, suggesting that psoralen may represent a novel therapeutic option for the prevention and treatment hepatocellular carcinoma.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100232en10.1186/s40659-019-0241-8
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Psoralen Hepatocellular carcinoma SMMC7721 cell Endoplasmic reticulum stress Apoptosis
spellingShingle	Psoralen Hepatocellular carcinoma SMMC7721 cell Endoplasmic reticulum stress Apoptosis Wang,Xiaomin Peng,Peike Pan,Zhiqiang Fang,Zhaoqin Lu,Wenli Liu,Xiaomei Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells
description	Abstract Background: Psoralen is a coumarin-like and coumarin-related benzofuran glycoside, which is a commonly used traditional Chinese medicine to treat patients with kidney and spleen-yang deficiency symptom. Psoralen has been reported to show estrogen-like activity, antioxidant activity, osteoblastic proliferation accelerating activity, antitumor effects and antibacterial activity. However, the antitumor mechanism of psoralen is not fully understood. This study aimed to investigate the therapeutic efficacy of psoralen in human hepatoma cell line SMMC7721 and the mechanism of antitumor effects. Results: Psoralen inhibited proliferation of SMMC7721 in a dose- and time-dependent manner, and promoted apoptosis. Further, psoralen activated the ER stress signal pathway, including the expansion of endoplasmic reticulum, increasing the mRNA levels of GRP78, DDIT3, ATF4, XBP1, GADD34 and the protein levels of GDF15, GRP78, IRE1α, XBP-1s in a time-dependent manner. Psoralen induces cell cycle arrest at G1 phase by enhancing CyclinD1 and reducing CyclinE1 expression. Moreover, TUDC couldn't inhibit the psoralen-induced ER stress in SMMC7721 cells. Conclusions: Psoralen can inhibit the proliferation of SMMC7721 cells and induce ER stress response to induce cell apoptosis, suggesting that psoralen may represent a novel therapeutic option for the prevention and treatment hepatocellular carcinoma.
author	Wang,Xiaomin Peng,Peike Pan,Zhiqiang Fang,Zhaoqin Lu,Wenli Liu,Xiaomei
author_facet	Wang,Xiaomin Peng,Peike Pan,Zhiqiang Fang,Zhaoqin Lu,Wenli Liu,Xiaomei
author_sort	Wang,Xiaomin
title	Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells
title_short	Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells
title_full	Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells
title_fullStr	Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells
title_full_unstemmed	Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells
title_sort	psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human smmc7721 hepatoma cells
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100232
work_keys_str_mv	AT wangxiaomin psoraleninhibitsmalignantproliferationandinducesapoptosisthroughtriggeringendoplasmicreticulumstressinhumansmmc7721hepatomacells AT pengpeike psoraleninhibitsmalignantproliferationandinducesapoptosisthroughtriggeringendoplasmicreticulumstressinhumansmmc7721hepatomacells AT panzhiqiang psoraleninhibitsmalignantproliferationandinducesapoptosisthroughtriggeringendoplasmicreticulumstressinhumansmmc7721hepatomacells AT fangzhaoqin psoraleninhibitsmalignantproliferationandinducesapoptosisthroughtriggeringendoplasmicreticulumstressinhumansmmc7721hepatomacells AT luwenli psoraleninhibitsmalignantproliferationandinducesapoptosisthroughtriggeringendoplasmicreticulumstressinhumansmmc7721hepatomacells AT liuxiaomei psoraleninhibitsmalignantproliferationandinducesapoptosisthroughtriggeringendoplasmicreticulumstressinhumansmmc7721hepatomacells
_version_	1718441592030756864

Psoralen inhibits malignant proliferation and induces apoptosis through triggering endoplasmic reticulum stress in human SMMC7721 hepatoma cells

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