miR-29b-3p promotes progression of MDA-mB-231 triple-negative breast cancer cells through downregulating TRAF3

Abstract Background: Breast cancer is the second common malignant cancer among females worldwide. Accumulating studies have indicated that deregulation of miRNA expression in breast cancer will contribute to tumorigenesis and form different cancer subtypes. However, the reported studies on miR-29b-...

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Autores principales:	Zhang,Bao, Shetti,Dattatrya, Fan,Conghui, Wei,Kun
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	miR-29b-3p Triple negative breast cancer Cytoskeleton TRAF3 NF-κB
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100235
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spelling	oai:scielo:S0716-976020190001002352019-10-10miR-29b-3p promotes progression of MDA-mB-231 triple-negative breast cancer cells through downregulating TRAF3Zhang,BaoShetti,DattatryaFan,ConghuiWei,Kun miR-29b-3p Triple negative breast cancer Cytoskeleton TRAF3 NF-κB Abstract Background: Breast cancer is the second common malignant cancer among females worldwide. Accumulating studies have indicated that deregulation of miRNA expression in breast cancer will contribute to tumorigenesis and form different cancer subtypes. However, the reported studies on miR-29b-3p-regulated breast cancer are limited so far. Herein, we investigated the role and mechanism of miR-29b-3p in the triple negative breast cancer cell line MDA-MB-231. Methods: The relative miR-29b-3p expression in different breast cancer cell lines were determined by qRT-PCR. CCK8 and colony formation assay were used to determine the influence of miR-29b-3p on cell proliferation. Migration assay and invasion assay were performed for cell migration and invasion respectively. To study the cell integrity immunofluorescence was performed. TUNEL assay, flow cytometry assay, hoechst staining and western blot were conducted to determine the influence of miR-29b-3p inhibitor on cell apoptosis. TRAF3 was found to be the target gene of miR-29b-3p using bioinformatics predictions. Dual-luciferase assay was performed to determine the relative luciferase activity in NC, miR-29b-3p mimic, miR-29b-3p inhibitor with TRAF3 3’-UTR wt or TRAF3 3’-UTR mt reporter plasmids. The proteins expression of NF-κB signaling pathway in MDA-MB-231 after transfection with NC, miR-29b-3p mimic, miR-29b-3p inhibitor were determined by western blot. Results: The miR-29b-3p expression was significantly increased in MDA-MB-231 compare with MCF-10A. miR-29b-3p inhibitor reduced the cell viability of MDA-MB-231 and inhibited cell migration and invasion. Cell cytoskeleton integrity destroyed after miR-29b-3p inhibitor treatment. Furthermore, we identified the mechanism and found miR-29b-3p targets the TRAF3 and activates NF-κB signaling pathway. Conclusions: From the above studies, our results indicated that miR-29b-3p acts as a promoter for the development of MDA-MB-231.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100235en10.1186/s40659-019-0245-4
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	miR-29b-3p Triple negative breast cancer Cytoskeleton TRAF3 NF-κB
spellingShingle	miR-29b-3p Triple negative breast cancer Cytoskeleton TRAF3 NF-κB Zhang,Bao Shetti,Dattatrya Fan,Conghui Wei,Kun miR-29b-3p promotes progression of MDA-mB-231 triple-negative breast cancer cells through downregulating TRAF3
description	Abstract Background: Breast cancer is the second common malignant cancer among females worldwide. Accumulating studies have indicated that deregulation of miRNA expression in breast cancer will contribute to tumorigenesis and form different cancer subtypes. However, the reported studies on miR-29b-3p-regulated breast cancer are limited so far. Herein, we investigated the role and mechanism of miR-29b-3p in the triple negative breast cancer cell line MDA-MB-231. Methods: The relative miR-29b-3p expression in different breast cancer cell lines were determined by qRT-PCR. CCK8 and colony formation assay were used to determine the influence of miR-29b-3p on cell proliferation. Migration assay and invasion assay were performed for cell migration and invasion respectively. To study the cell integrity immunofluorescence was performed. TUNEL assay, flow cytometry assay, hoechst staining and western blot were conducted to determine the influence of miR-29b-3p inhibitor on cell apoptosis. TRAF3 was found to be the target gene of miR-29b-3p using bioinformatics predictions. Dual-luciferase assay was performed to determine the relative luciferase activity in NC, miR-29b-3p mimic, miR-29b-3p inhibitor with TRAF3 3’-UTR wt or TRAF3 3’-UTR mt reporter plasmids. The proteins expression of NF-κB signaling pathway in MDA-MB-231 after transfection with NC, miR-29b-3p mimic, miR-29b-3p inhibitor were determined by western blot. Results: The miR-29b-3p expression was significantly increased in MDA-MB-231 compare with MCF-10A. miR-29b-3p inhibitor reduced the cell viability of MDA-MB-231 and inhibited cell migration and invasion. Cell cytoskeleton integrity destroyed after miR-29b-3p inhibitor treatment. Furthermore, we identified the mechanism and found miR-29b-3p targets the TRAF3 and activates NF-κB signaling pathway. Conclusions: From the above studies, our results indicated that miR-29b-3p acts as a promoter for the development of MDA-MB-231.
author	Zhang,Bao Shetti,Dattatrya Fan,Conghui Wei,Kun
author_facet	Zhang,Bao Shetti,Dattatrya Fan,Conghui Wei,Kun
author_sort	Zhang,Bao
title	miR-29b-3p promotes progression of MDA-mB-231 triple-negative breast cancer cells through downregulating TRAF3
title_short	miR-29b-3p promotes progression of MDA-mB-231 triple-negative breast cancer cells through downregulating TRAF3
title_full	miR-29b-3p promotes progression of MDA-mB-231 triple-negative breast cancer cells through downregulating TRAF3
title_fullStr	miR-29b-3p promotes progression of MDA-mB-231 triple-negative breast cancer cells through downregulating TRAF3
title_full_unstemmed	miR-29b-3p promotes progression of MDA-mB-231 triple-negative breast cancer cells through downregulating TRAF3
title_sort	mir-29b-3p promotes progression of mda-mb-231 triple-negative breast cancer cells through downregulating traf3
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100235
work_keys_str_mv	AT zhangbao mir29b3ppromotesprogressionofmdamb231triplenegativebreastcancercellsthroughdownregulatingtraf3 AT shettidattatrya mir29b3ppromotesprogressionofmdamb231triplenegativebreastcancercellsthroughdownregulatingtraf3 AT fanconghui mir29b3ppromotesprogressionofmdamb231triplenegativebreastcancercellsthroughdownregulatingtraf3 AT weikun mir29b3ppromotesprogressionofmdamb231triplenegativebreastcancercellsthroughdownregulatingtraf3
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miR-29b-3p promotes progression of MDA-mB-231 triple-negative breast cancer cells through downregulating TRAF3

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