A novel hydrazide compound exerts anti-metastatic effect against breast cancer

Abstract Background: There are currently a number of barriers hindering the successful treatment of breast cancer, including the metastatic spread of cancer cells. In looking for new anticancer agents, we reported two novel hydrazide derivatives with anti-cancer activity in human breast cancer cell...

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Autores principales: Dehghani,Soudeh, Kooshafar,Zahra, Almasirad,Ali, Tahmasvand,Raheleh, Moayer,Fariborz, Muhammadnejad,Ahad, Shafiee,Samira, Salimi,Mona
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2019
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100237
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spelling oai:scielo:S0716-976020190001002372019-10-10A novel hydrazide compound exerts anti-metastatic effect against breast cancerDehghani,SoudehKooshafar,ZahraAlmasirad,AliTahmasvand,RahelehMoayer,FariborzMuhammadnejad,AhadShafiee,SamiraSalimi,Mona Metastasis Breast cancer Matrigel Immunohistochemistry Soft agar Abstract Background: There are currently a number of barriers hindering the successful treatment of breast cancer, including the metastatic spread of cancer cells. In looking for new anticancer agents, we reported two novel hydrazide derivatives with anti-cancer activity in human breast cancer cells. The current study aims to explore the therapeutic potential of the most effective one, N’-((5-nitrothiophen-2-yl)methylene)-2-(phenylthio)benzohydrazide (compound B), on metastatic breast cancer, which is resistant to available chemotherapeutics. Methods: 4T1 mammary carcinoma cells were inoculated into the fat pad mammary of 5-7-week-old female BALB/c mice and then the effective compound was intraperitoneally administered for 4 weeks. Proliferation index and angiogenesis in tumor and lung tissues were examined with immunohistochemistry. In vitro assessments were also carried out to evaluate the effect of the compound on invasion of MDA-MB-231 cells. Results: Our results demonstrated that this effective derivative significantly inhibited invasion of MDA-MB-231 cells in vitro as shown by Matrigel assay and quantitative real-time method for MMP-9 expression after 48 h of treatment. Daily administration of the compound suppressed the growth of primary tumor and its metastasis to lung, which was confirmed by H&E experiment at a dose of 1 mg/kg in a well-known metastatic model of 4T1 breast cancer in syngeneic BALB/c mice. These outcomes were supported by the immunohistochemical examinations of the tumor and lung tissues of mice. Tumors and lungs in mice treated with the effective compound showed a reduced proliferation index and a smaller microvessel density compared to the control. Conclusion: This study highlights an anti-metastatic role for a novel hydrazide derivative in both in vitro and in vivo models of breast cancer.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100237en10.1186/s40659-019-0247-2
institution Scielo Chile
collection Scielo Chile
language English
topic Metastasis
Breast cancer
Matrigel
Immunohistochemistry
Soft agar
spellingShingle Metastasis
Breast cancer
Matrigel
Immunohistochemistry
Soft agar
Dehghani,Soudeh
Kooshafar,Zahra
Almasirad,Ali
Tahmasvand,Raheleh
Moayer,Fariborz
Muhammadnejad,Ahad
Shafiee,Samira
Salimi,Mona
A novel hydrazide compound exerts anti-metastatic effect against breast cancer
description Abstract Background: There are currently a number of barriers hindering the successful treatment of breast cancer, including the metastatic spread of cancer cells. In looking for new anticancer agents, we reported two novel hydrazide derivatives with anti-cancer activity in human breast cancer cells. The current study aims to explore the therapeutic potential of the most effective one, N’-((5-nitrothiophen-2-yl)methylene)-2-(phenylthio)benzohydrazide (compound B), on metastatic breast cancer, which is resistant to available chemotherapeutics. Methods: 4T1 mammary carcinoma cells were inoculated into the fat pad mammary of 5-7-week-old female BALB/c mice and then the effective compound was intraperitoneally administered for 4 weeks. Proliferation index and angiogenesis in tumor and lung tissues were examined with immunohistochemistry. In vitro assessments were also carried out to evaluate the effect of the compound on invasion of MDA-MB-231 cells. Results: Our results demonstrated that this effective derivative significantly inhibited invasion of MDA-MB-231 cells in vitro as shown by Matrigel assay and quantitative real-time method for MMP-9 expression after 48 h of treatment. Daily administration of the compound suppressed the growth of primary tumor and its metastasis to lung, which was confirmed by H&E experiment at a dose of 1 mg/kg in a well-known metastatic model of 4T1 breast cancer in syngeneic BALB/c mice. These outcomes were supported by the immunohistochemical examinations of the tumor and lung tissues of mice. Tumors and lungs in mice treated with the effective compound showed a reduced proliferation index and a smaller microvessel density compared to the control. Conclusion: This study highlights an anti-metastatic role for a novel hydrazide derivative in both in vitro and in vivo models of breast cancer.
author Dehghani,Soudeh
Kooshafar,Zahra
Almasirad,Ali
Tahmasvand,Raheleh
Moayer,Fariborz
Muhammadnejad,Ahad
Shafiee,Samira
Salimi,Mona
author_facet Dehghani,Soudeh
Kooshafar,Zahra
Almasirad,Ali
Tahmasvand,Raheleh
Moayer,Fariborz
Muhammadnejad,Ahad
Shafiee,Samira
Salimi,Mona
author_sort Dehghani,Soudeh
title A novel hydrazide compound exerts anti-metastatic effect against breast cancer
title_short A novel hydrazide compound exerts anti-metastatic effect against breast cancer
title_full A novel hydrazide compound exerts anti-metastatic effect against breast cancer
title_fullStr A novel hydrazide compound exerts anti-metastatic effect against breast cancer
title_full_unstemmed A novel hydrazide compound exerts anti-metastatic effect against breast cancer
title_sort novel hydrazide compound exerts anti-metastatic effect against breast cancer
publisher Sociedad de Biología de Chile
publishDate 2019
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100237
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