Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)

Abstract Background: Di-N-butyl-phthalate (DBP) is an endocrine disrupting substance. We investigated the adverse effect of DBP on testis of male rat and reveal its potential mechanism of MAPK signaling pathway involved this effect in vivo and in vitro. Gonadal hormone, sperm quality, morphological...

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Autores principales:	Wang,Hongyan, Zhou,Weipeng, Zhang,Jing, Li,Huan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	Di-N-butyl-phthalate Testis Sertoli cells Mitogen-activated protein kinase
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100238
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spelling	oai:scielo:S0716-976020190001002382019-10-10Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)Wang,HongyanZhou,WeipengZhang,JingLi,Huan Di-N-butyl-phthalate Testis Sertoli cells Mitogen-activated protein kinase Abstract Background: Di-N-butyl-phthalate (DBP) is an endocrine disrupting substance. We investigated the adverse effect of DBP on testis of male rat and reveal its potential mechanism of MAPK signaling pathway involved this effect in vivo and in vitro. Gonadal hormone, sperm quality, morphological change and the activation status of JNK, ERK1/2 and p38 was determined in vivo. Primary Sertoli cell was established and cultivated with JNK, ERK1/2 inhibitors, then determine the cell viability, apoptosis and the expression of p-JNK, p-ERK1/2. Data in this study were presented as mean ± SD and determined by one-way analysis of variance (ANOVA) followed by Bonferroni's test. Difference was considered statistically significant at P < 0.05. Results: In vivo experiment, DBP impaired the normal structure of testicular tissue, reduced testosterone levels in blood serum, decreased sperm count and increased sperm abnormality, p-ERK1/2 and p-JNK in rat testicular tissue increased in a dose-dependent manner. In vitro studies, DBP could decrease the viability of Sertoli cells and increase p-ERK1/2 and p-JNK. Cell apoptosis in SP600125 + DBP group was significantly lower than in DBP group (P < 0.05). p-JNK was not significantly decreased in SP600125 + DBP group, while p-ERK1/2 was significantly decreased in U0126 + DBP group. Conclusions: These results suggest that DBP can lead to testicular damage and the activation of ERK1/2 and JNK pathways, the JNK signaling pathway may be primarily associated with its effect.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100238en10.1186/s40659-019-0248-1
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Di-N-butyl-phthalate Testis Sertoli cells Mitogen-activated protein kinase
spellingShingle	Di-N-butyl-phthalate Testis Sertoli cells Mitogen-activated protein kinase Wang,Hongyan Zhou,Weipeng Zhang,Jing Li,Huan Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)
description	Abstract Background: Di-N-butyl-phthalate (DBP) is an endocrine disrupting substance. We investigated the adverse effect of DBP on testis of male rat and reveal its potential mechanism of MAPK signaling pathway involved this effect in vivo and in vitro. Gonadal hormone, sperm quality, morphological change and the activation status of JNK, ERK1/2 and p38 was determined in vivo. Primary Sertoli cell was established and cultivated with JNK, ERK1/2 inhibitors, then determine the cell viability, apoptosis and the expression of p-JNK, p-ERK1/2. Data in this study were presented as mean ± SD and determined by one-way analysis of variance (ANOVA) followed by Bonferroni's test. Difference was considered statistically significant at P < 0.05. Results: In vivo experiment, DBP impaired the normal structure of testicular tissue, reduced testosterone levels in blood serum, decreased sperm count and increased sperm abnormality, p-ERK1/2 and p-JNK in rat testicular tissue increased in a dose-dependent manner. In vitro studies, DBP could decrease the viability of Sertoli cells and increase p-ERK1/2 and p-JNK. Cell apoptosis in SP600125 + DBP group was significantly lower than in DBP group (P < 0.05). p-JNK was not significantly decreased in SP600125 + DBP group, while p-ERK1/2 was significantly decreased in U0126 + DBP group. Conclusions: These results suggest that DBP can lead to testicular damage and the activation of ERK1/2 and JNK pathways, the JNK signaling pathway may be primarily associated with its effect.
author	Wang,Hongyan Zhou,Weipeng Zhang,Jing Li,Huan
author_facet	Wang,Hongyan Zhou,Weipeng Zhang,Jing Li,Huan
author_sort	Wang,Hongyan
title	Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)
title_short	Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)
title_full	Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)
title_fullStr	Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)
title_full_unstemmed	Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)
title_sort	role of jnk and erk1/2 mapk signaling pathway in testicular injury of rats induced by di-n-butyl-phthalate (dbp)
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100238
work_keys_str_mv	AT wanghongyan roleofjnkanderk12mapksignalingpathwayintesticularinjuryofratsinducedbydinbutylphthalatedbp AT zhouweipeng roleofjnkanderk12mapksignalingpathwayintesticularinjuryofratsinducedbydinbutylphthalatedbp AT zhangjing roleofjnkanderk12mapksignalingpathwayintesticularinjuryofratsinducedbydinbutylphthalatedbp AT lihuan roleofjnkanderk12mapksignalingpathwayintesticularinjuryofratsinducedbydinbutylphthalatedbp
_version_	1718441593796558848

Role of JNK and ERK1/2 MAPK signaling pathway in testicular injury of rats induced by di-N-butyl-phthalate (DBP)

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