Light-induced injury in mouse embryos revealed by single-cell RNA sequencing

Abstract Background: Light exposure is a common stress factor in in vitro manipulation of embryos in the reproductive center. Many studies have shown the deleterious effects of high-intensity light exposure in different animal embryos. However, no transcriptomic studies have explored the light-indu...

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Autores principales:	Lv,Bo, Liu,Chaojie, Chen,Yu, Qi,Lingbin, Wang,Lu, Ji,Yazhong, Xue,Zhigang
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	Light exposure Light-induced response In vitro culture Single-cell RNA-seq
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100244
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spelling	oai:scielo:S0716-976020190001002442019-10-10Light-induced injury in mouse embryos revealed by single-cell RNA sequencingLv,BoLiu,ChaojieChen,YuQi,LingbinWang,LuJi,YazhongXue,Zhigang Light exposure Light-induced response In vitro culture Single-cell RNA-seq Abstract Background: Light exposure is a common stress factor in in vitro manipulation of embryos in the reproductive center. Many studies have shown the deleterious effects of high-intensity light exposure in different animal embryos. However, no transcriptomic studies have explored the light-induced injury and response in preimplantation embryos. Results: Here, we adopt different time-courses and illumination intensities to treat mouse embryos at the 2-cell stage and evaluate their effects on blastulation. Meanwhile, single-cell transcriptomes from the 2-cell to blastocyst stage were analyzed after high-intensity light exposure. These data show that cells at each embryonic stage can be categorized into different light conditions. Further analyses of differentially expressed genes and GO terms revealed the light-induced injury as well as the potential repair response after high-intensity lighting. Maternal-to-zygote transition is also affected by the failure to remove maternal RNAs and deactivate zygotic genome expression. Conclusion: Our work revealed an integrated response to high-intensity lighting, involving morphological changes, long-lasting injury effects, and intracellular damage repair mechanisms.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100244en10.1186/s40659-019-0256-1
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Light exposure Light-induced response In vitro culture Single-cell RNA-seq
spellingShingle	Light exposure Light-induced response In vitro culture Single-cell RNA-seq Lv,Bo Liu,Chaojie Chen,Yu Qi,Lingbin Wang,Lu Ji,Yazhong Xue,Zhigang Light-induced injury in mouse embryos revealed by single-cell RNA sequencing
description	Abstract Background: Light exposure is a common stress factor in in vitro manipulation of embryos in the reproductive center. Many studies have shown the deleterious effects of high-intensity light exposure in different animal embryos. However, no transcriptomic studies have explored the light-induced injury and response in preimplantation embryos. Results: Here, we adopt different time-courses and illumination intensities to treat mouse embryos at the 2-cell stage and evaluate their effects on blastulation. Meanwhile, single-cell transcriptomes from the 2-cell to blastocyst stage were analyzed after high-intensity light exposure. These data show that cells at each embryonic stage can be categorized into different light conditions. Further analyses of differentially expressed genes and GO terms revealed the light-induced injury as well as the potential repair response after high-intensity lighting. Maternal-to-zygote transition is also affected by the failure to remove maternal RNAs and deactivate zygotic genome expression. Conclusion: Our work revealed an integrated response to high-intensity lighting, involving morphological changes, long-lasting injury effects, and intracellular damage repair mechanisms.
author	Lv,Bo Liu,Chaojie Chen,Yu Qi,Lingbin Wang,Lu Ji,Yazhong Xue,Zhigang
author_facet	Lv,Bo Liu,Chaojie Chen,Yu Qi,Lingbin Wang,Lu Ji,Yazhong Xue,Zhigang
author_sort	Lv,Bo
title	Light-induced injury in mouse embryos revealed by single-cell RNA sequencing
title_short	Light-induced injury in mouse embryos revealed by single-cell RNA sequencing
title_full	Light-induced injury in mouse embryos revealed by single-cell RNA sequencing
title_fullStr	Light-induced injury in mouse embryos revealed by single-cell RNA sequencing
title_full_unstemmed	Light-induced injury in mouse embryos revealed by single-cell RNA sequencing
title_sort	light-induced injury in mouse embryos revealed by single-cell rna sequencing
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100244
work_keys_str_mv	AT lvbo lightinducedinjuryinmouseembryosrevealedbysinglecellrnasequencing AT liuchaojie lightinducedinjuryinmouseembryosrevealedbysinglecellrnasequencing AT chenyu lightinducedinjuryinmouseembryosrevealedbysinglecellrnasequencing AT qilingbin lightinducedinjuryinmouseembryosrevealedbysinglecellrnasequencing AT wanglu lightinducedinjuryinmouseembryosrevealedbysinglecellrnasequencing AT jiyazhong lightinducedinjuryinmouseembryosrevealedbysinglecellrnasequencing AT xuezhigang lightinducedinjuryinmouseembryosrevealedbysinglecellrnasequencing
_version_	1718441595311751168

Light-induced injury in mouse embryos revealed by single-cell RNA sequencing

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