Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells

Abstract Background: Epanorin (EP) is a secondary metabolite of the Acarospora lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cell...

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Autores principales: Palacios-Moreno,Juan, Rubio,Cecilia, Quilhot,Wanda, Cavieres,M. Fernanda, Peña,Eduardo de la, Quiñones,Natalia V., Díaz,Hugo, Carrión,Flavio, Henríquez-Roldán,Carlos F., Weinstein-Oppenheimer,Caroline R.
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2019
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100251
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spelling oai:scielo:S0716-976020190001002512019-12-02Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cellsPalacios-Moreno,JuanRubio,CeciliaQuilhot,WandaCavieres,M. FernandaPeña,Eduardo de laQuiñones,Natalia V.Díaz,HugoCarrión,FlavioHenríquez-Roldán,Carlos F.Weinstein-Oppenheimer,Caroline R. Epanorin Cancer Cytotoxicity Mutagenesis Cell cycle Apoptosis Abstract Background: Epanorin (EP) is a secondary metabolite of the Acarospora lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cells has not yet been explored. It has been hypothesized that EP inhibits cancer cell growth. MCF-7 breast cancer cells, normal fibroblasts, and the non-transformed HEK-293 cell line were exposed to increasing concentrations of EP, and proliferation was assessed by the sulforhodamine-B assay. Results: MCF-7 cells exposed to EP were examined for cell cycle progression using flow cytometry, and DNA fragmentation was examined using the TUNEL assay. In addition, EP's mutagenic activity was assessed using the Salmonella typhimurium reverse mutation assay. The data showed that EP inhibits proliferation of MCF-7 cells, and it induces cell cycle arrest in G0/G1 through a DNA fragmentation-independent mechanism. Furthermore, EP's lack of overt cytotoxicity in the normal cell line HEK-293 and human fibroblasts in cell culture is supported by the absence of mutagenic activity of EP. Conclusion: EP emerges as a suitable molecule for further studies as a potential antineoplastic agent.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100251en10.1186/s40659-019-0261-4
institution Scielo Chile
collection Scielo Chile
language English
topic Epanorin
Cancer
Cytotoxicity
Mutagenesis
Cell cycle
Apoptosis
spellingShingle Epanorin
Cancer
Cytotoxicity
Mutagenesis
Cell cycle
Apoptosis
Palacios-Moreno,Juan
Rubio,Cecilia
Quilhot,Wanda
Cavieres,M. Fernanda
Peña,Eduardo de la
Quiñones,Natalia V.
Díaz,Hugo
Carrión,Flavio
Henríquez-Roldán,Carlos F.
Weinstein-Oppenheimer,Caroline R.
Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells
description Abstract Background: Epanorin (EP) is a secondary metabolite of the Acarospora lichenic species. EP has been found in lichenic extracts with antimicrobial activity, and UV-absorption properties have been described for closely related molecules; however, its antiproliferative activity in cancer cells has not yet been explored. It has been hypothesized that EP inhibits cancer cell growth. MCF-7 breast cancer cells, normal fibroblasts, and the non-transformed HEK-293 cell line were exposed to increasing concentrations of EP, and proliferation was assessed by the sulforhodamine-B assay. Results: MCF-7 cells exposed to EP were examined for cell cycle progression using flow cytometry, and DNA fragmentation was examined using the TUNEL assay. In addition, EP's mutagenic activity was assessed using the Salmonella typhimurium reverse mutation assay. The data showed that EP inhibits proliferation of MCF-7 cells, and it induces cell cycle arrest in G0/G1 through a DNA fragmentation-independent mechanism. Furthermore, EP's lack of overt cytotoxicity in the normal cell line HEK-293 and human fibroblasts in cell culture is supported by the absence of mutagenic activity of EP. Conclusion: EP emerges as a suitable molecule for further studies as a potential antineoplastic agent.
author Palacios-Moreno,Juan
Rubio,Cecilia
Quilhot,Wanda
Cavieres,M. Fernanda
Peña,Eduardo de la
Quiñones,Natalia V.
Díaz,Hugo
Carrión,Flavio
Henríquez-Roldán,Carlos F.
Weinstein-Oppenheimer,Caroline R.
author_facet Palacios-Moreno,Juan
Rubio,Cecilia
Quilhot,Wanda
Cavieres,M. Fernanda
Peña,Eduardo de la
Quiñones,Natalia V.
Díaz,Hugo
Carrión,Flavio
Henríquez-Roldán,Carlos F.
Weinstein-Oppenheimer,Caroline R.
author_sort Palacios-Moreno,Juan
title Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells
title_short Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells
title_full Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells
title_fullStr Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells
title_full_unstemmed Epanorin, a lichen secondary metabolite, inhibits proliferation of MCF-7 breast cancer cells
title_sort epanorin, a lichen secondary metabolite, inhibits proliferation of mcf-7 breast cancer cells
publisher Sociedad de Biología de Chile
publishDate 2019
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100251
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