PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat

Abstract Background: ADP-glucose pyrophosphorylase (AGPase), the key enzyme in plant starch biosynthesis, is a heterotetramer composed of two identical large subunits and two identical small subunits. AGPase has plastidial and cytosolic isoforms in higher plants, whereas it is mainly detected in th...

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Autores principales:	Dong,Jie, Zheng,Yongxing, Fu,Yihan, Wang,Jinxi, Yuan,Shasha, Wang,Yonghua, Zhu,Qidi, Ou,Xingqi, Li,Gezi, Kang,Guozhang
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	AGPase BSMV-VIGS Protein disulfide isomerase Starch biosynthesis Triticum aestivum L. Yeast one-hybrid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100252
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spelling	oai:scielo:S0716-976020190001002522019-12-02PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheatDong,JieZheng,YongxingFu,YihanWang,JinxiYuan,ShashaWang,YonghuaZhu,QidiOu,XingqiLi,GeziKang,Guozhang AGPase BSMV-VIGS Protein disulfide isomerase Starch biosynthesis Triticum aestivum L. Yeast one-hybrid Abstract Background: ADP-glucose pyrophosphorylase (AGPase), the key enzyme in plant starch biosynthesis, is a heterotetramer composed of two identical large subunits and two identical small subunits. AGPase has plastidial and cytosolic isoforms in higher plants, whereas it is mainly detected in the cytosol of grain endosperms in cereal crops. Our previous results have shown that the expression of the TaAGPL1 gene, encoding the cytosolic large subunit of wheat AGPase, temporally coincides with the rate of starch accumulation and that its overexpression dramatically increases wheat AGPase activity and the rate of starch accumulation, suggesting an important role. Methods: In this study, we performed yeast one-hybrid screening using the promoter of the TaAGPL1 gene as bait and a wheat grain cDNA library as prey to screen out the upstream regulators of TaAGPL1 gene. And the barley stripe mosaic virus-induced gene-silencing (BSMV-VIGS) method was used to verify the functional characterization of the identified regulators in starch biosynthesis. Results: Disulfide isomerase 1-2 protein (TaPDIL1-2) was screened out, and its binding to the TaAGPL1-1D promoter was further verified using another yeast one-hybrid screen. Transiently silenced wheat plants of the TaPDIL1-2 gene were obtained by using BSMV-VIGS method under field conditions. In grains of BSMV-VIGS-TaPDIL1-2-silenced wheat plants, the TaAGPL1 gene transcription levels, grain starch contents, and 1000-kernel weight also significantly increased. Conclusions: As important chaperones involved in oxidative protein folding, PDIL proteins have been reported to form hetero-dimers with some transcription factors, and thus, our results suggested that TaPDIL1-2 protein could indirectly and negatively regulate the expression of the TaAGPL1 gene and function in starch biosynthesis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100252en10.1186/s40659-019-0263-2
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	AGPase BSMV-VIGS Protein disulfide isomerase Starch biosynthesis Triticum aestivum L. Yeast one-hybrid
spellingShingle	AGPase BSMV-VIGS Protein disulfide isomerase Starch biosynthesis Triticum aestivum L. Yeast one-hybrid Dong,Jie Zheng,Yongxing Fu,Yihan Wang,Jinxi Yuan,Shasha Wang,Yonghua Zhu,Qidi Ou,Xingqi Li,Gezi Kang,Guozhang PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat
description	Abstract Background: ADP-glucose pyrophosphorylase (AGPase), the key enzyme in plant starch biosynthesis, is a heterotetramer composed of two identical large subunits and two identical small subunits. AGPase has plastidial and cytosolic isoforms in higher plants, whereas it is mainly detected in the cytosol of grain endosperms in cereal crops. Our previous results have shown that the expression of the TaAGPL1 gene, encoding the cytosolic large subunit of wheat AGPase, temporally coincides with the rate of starch accumulation and that its overexpression dramatically increases wheat AGPase activity and the rate of starch accumulation, suggesting an important role. Methods: In this study, we performed yeast one-hybrid screening using the promoter of the TaAGPL1 gene as bait and a wheat grain cDNA library as prey to screen out the upstream regulators of TaAGPL1 gene. And the barley stripe mosaic virus-induced gene-silencing (BSMV-VIGS) method was used to verify the functional characterization of the identified regulators in starch biosynthesis. Results: Disulfide isomerase 1-2 protein (TaPDIL1-2) was screened out, and its binding to the TaAGPL1-1D promoter was further verified using another yeast one-hybrid screen. Transiently silenced wheat plants of the TaPDIL1-2 gene were obtained by using BSMV-VIGS method under field conditions. In grains of BSMV-VIGS-TaPDIL1-2-silenced wheat plants, the TaAGPL1 gene transcription levels, grain starch contents, and 1000-kernel weight also significantly increased. Conclusions: As important chaperones involved in oxidative protein folding, PDIL proteins have been reported to form hetero-dimers with some transcription factors, and thus, our results suggested that TaPDIL1-2 protein could indirectly and negatively regulate the expression of the TaAGPL1 gene and function in starch biosynthesis.
author	Dong,Jie Zheng,Yongxing Fu,Yihan Wang,Jinxi Yuan,Shasha Wang,Yonghua Zhu,Qidi Ou,Xingqi Li,Gezi Kang,Guozhang
author_facet	Dong,Jie Zheng,Yongxing Fu,Yihan Wang,Jinxi Yuan,Shasha Wang,Yonghua Zhu,Qidi Ou,Xingqi Li,Gezi Kang,Guozhang
author_sort	Dong,Jie
title	PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat
title_short	PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat
title_full	PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat
title_fullStr	PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat
title_full_unstemmed	PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat
title_sort	pdil1-2 can indirectly and negatively regulate expression of the agpl1 gene in bread wheat
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100252
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_version_	1718441597198139392

PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat

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