Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis

Abstract Background: Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide, which seriously affects human health. Gramicidin is a short peptide antibiotic which could be used for treating infection induced by bacteria or fungi. However, the anti-cancer effect of gra...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen,Tingting, Wang,Yong, Yang,Yang, Yu,Kaikai, Cao,Xiangliao, Su,Fang, Xu,Huanbai, Peng,Yongde, Hu,Yudong, Qian,Feng, Wang,Zishu
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2019
Materias:	Gramicidin Proliferation Apoptosis Cell cycle arrest SGC-7901
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100253
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602019000100253
record_format	dspace
spelling	oai:scielo:S0716-976020190001002532019-12-02Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosisChen,TingtingWang,YongYang,YangYu,KaikaiCao,XiangliaoSu,FangXu,HuanbaiPeng,YongdeHu,YudongQian,FengWang,Zishu Gramicidin Proliferation Apoptosis Cell cycle arrest SGC-7901 Abstract Background: Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide, which seriously affects human health. Gramicidin is a short peptide antibiotic which could be used for treating infection induced by bacteria or fungi. However, the anti-cancer effect of gramicidin on gastric cancer cells and its underlying mechanism remains largely unknown. Results: Gastric cancer cells SGC-7901, BGC-823 and normal gastric mucosal cells GES-1 were treated with different concentrations of gramicidin respectively. The results of CCK-8 experiment revealed cellular toxicity of gramicidin to cancer cells while cell colony formation assay showed that gramicidin significantly inhibited the proliferation of gastric cancer cells, but had little effect on normal gastric mucosal cells. In addition, the wound healing assay showed that gramicidin inhibited the migration of SGC-7901 cell. Meanwhile, apoptosis and cell cycle analysis revealed that gramicidin induced cell apoptosis with G2/M cell cycle inhibition. Furthermore, western blot analysis demonstrated that gramicidin down-regulated the expression of cyclinD1 and Bcl-2 as well as the FoxO1 phosphorylation. Conclusions: The current study illustrated the anti-tumor activity of gramicidin on gastric cancer cells, providing a possibility for gramicidin to be applied in clinical practice for the treatment of gastric cancer.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.52 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100253en10.1186/s40659-019-0264-1
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gramicidin Proliferation Apoptosis Cell cycle arrest SGC-7901
spellingShingle	Gramicidin Proliferation Apoptosis Cell cycle arrest SGC-7901 Chen,Tingting Wang,Yong Yang,Yang Yu,Kaikai Cao,Xiangliao Su,Fang Xu,Huanbai Peng,Yongde Hu,Yudong Qian,Feng Wang,Zishu Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis
description	Abstract Background: Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide, which seriously affects human health. Gramicidin is a short peptide antibiotic which could be used for treating infection induced by bacteria or fungi. However, the anti-cancer effect of gramicidin on gastric cancer cells and its underlying mechanism remains largely unknown. Results: Gastric cancer cells SGC-7901, BGC-823 and normal gastric mucosal cells GES-1 were treated with different concentrations of gramicidin respectively. The results of CCK-8 experiment revealed cellular toxicity of gramicidin to cancer cells while cell colony formation assay showed that gramicidin significantly inhibited the proliferation of gastric cancer cells, but had little effect on normal gastric mucosal cells. In addition, the wound healing assay showed that gramicidin inhibited the migration of SGC-7901 cell. Meanwhile, apoptosis and cell cycle analysis revealed that gramicidin induced cell apoptosis with G2/M cell cycle inhibition. Furthermore, western blot analysis demonstrated that gramicidin down-regulated the expression of cyclinD1 and Bcl-2 as well as the FoxO1 phosphorylation. Conclusions: The current study illustrated the anti-tumor activity of gramicidin on gastric cancer cells, providing a possibility for gramicidin to be applied in clinical practice for the treatment of gastric cancer.
author	Chen,Tingting Wang,Yong Yang,Yang Yu,Kaikai Cao,Xiangliao Su,Fang Xu,Huanbai Peng,Yongde Hu,Yudong Qian,Feng Wang,Zishu
author_facet	Chen,Tingting Wang,Yong Yang,Yang Yu,Kaikai Cao,Xiangliao Su,Fang Xu,Huanbai Peng,Yongde Hu,Yudong Qian,Feng Wang,Zishu
author_sort	Chen,Tingting
title	Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis
title_short	Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis
title_full	Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis
title_fullStr	Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis
title_full_unstemmed	Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis
title_sort	gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis
publisher	Sociedad de Biología de Chile
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602019000100253
work_keys_str_mv	AT chentingting gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis AT wangyong gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis AT yangyang gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis AT yukaikai gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis AT caoxiangliao gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis AT sufang gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis AT xuhuanbai gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis AT pengyongde gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis AT huyudong gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis AT qianfeng gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis AT wangzishu gramicidininhibitshumangastriccancercellproliferationcellcycleandinducedapoptosis
_version_	1718441597516906496

Gramicidin inhibits human gastric cancer cell proliferation, cell cycle and induced apoptosis

Ejemplares similares