Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins

Abstract Background: The pellicle, the acellular organic material deposited on the surface of tooth enamel, has been thought to be derived from saliva. In this study, protein compositions of the pellicle, gingival crevicular fluid, and saliva collected from healthy adults were compared to elucidate...

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Autores principales:	Odanaka,Hibiki, Obama,Takashi, Sawada,Naoko, Sugano,Marika, Itabe,Hiroyuki, Yamamoto,Matsuo
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	Pellicle Saliva Gingival crevicular fluid Proteome Isobaric tag labeling Serotransferrin Cystatin Alpha-amylase
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100202
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spelling	oai:scielo:S0716-976020200001002022020-06-25Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteinsOdanaka,HibikiObama,TakashiSawada,NaokoSugano,MarikaItabe,HiroyukiYamamoto,Matsuo Pellicle Saliva Gingival crevicular fluid Proteome Isobaric tag labeling Serotransferrin Cystatin Alpha-amylase Abstract Background: The pellicle, the acellular organic material deposited on the surface of tooth enamel, has been thought to be derived from saliva. In this study, protein compositions of the pellicle, gingival crevicular fluid, and saliva collected from healthy adults were compared to elucidate the origin of pellicle proteins. Results: The pellicle, gingival crevicular fluid, and saliva from the parotid gland or mixed gland were collected; subsequently, protein expression in samples from the respective individual was compared by SDS-PAGE and mass spectrometry. Following SDS-PAGE, proteins in the major bands were identified by mass spectrometry. The band pattern of pellicle proteins appeared different from those of gingival crevicular fluid, or saliva samples. Using mass spectrometry, 13 proteins in these samples were identified. The relative abundance of the proteins was quantitatively analyzed using mass spectrometry coupled with stable isotope labeling and by western blot. Cystatin S and α-amylase detected in pellicle were enriched in saliva samples, but not in gingival crevicular fluid, by western blot, and their abundance ratios were high in saliva and low in gingival crevicular fluid when analyzed by stable isotope labeling. Serotransferrin, however, was found only in the pellicle and gingival crevicular fluid by western blot and its abundance ratio was low in saliva. Conclusions: Our study revealed that the gingival crevicular fluid appears to contribute to pellicle formation in addition to saliva.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100202en10.1186/s40659-020-0271-2
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Pellicle Saliva Gingival crevicular fluid Proteome Isobaric tag labeling Serotransferrin Cystatin Alpha-amylase
spellingShingle	Pellicle Saliva Gingival crevicular fluid Proteome Isobaric tag labeling Serotransferrin Cystatin Alpha-amylase Odanaka,Hibiki Obama,Takashi Sawada,Naoko Sugano,Marika Itabe,Hiroyuki Yamamoto,Matsuo Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins
description	Abstract Background: The pellicle, the acellular organic material deposited on the surface of tooth enamel, has been thought to be derived from saliva. In this study, protein compositions of the pellicle, gingival crevicular fluid, and saliva collected from healthy adults were compared to elucidate the origin of pellicle proteins. Results: The pellicle, gingival crevicular fluid, and saliva from the parotid gland or mixed gland were collected; subsequently, protein expression in samples from the respective individual was compared by SDS-PAGE and mass spectrometry. Following SDS-PAGE, proteins in the major bands were identified by mass spectrometry. The band pattern of pellicle proteins appeared different from those of gingival crevicular fluid, or saliva samples. Using mass spectrometry, 13 proteins in these samples were identified. The relative abundance of the proteins was quantitatively analyzed using mass spectrometry coupled with stable isotope labeling and by western blot. Cystatin S and α-amylase detected in pellicle were enriched in saliva samples, but not in gingival crevicular fluid, by western blot, and their abundance ratios were high in saliva and low in gingival crevicular fluid when analyzed by stable isotope labeling. Serotransferrin, however, was found only in the pellicle and gingival crevicular fluid by western blot and its abundance ratio was low in saliva. Conclusions: Our study revealed that the gingival crevicular fluid appears to contribute to pellicle formation in addition to saliva.
author	Odanaka,Hibiki Obama,Takashi Sawada,Naoko Sugano,Marika Itabe,Hiroyuki Yamamoto,Matsuo
author_facet	Odanaka,Hibiki Obama,Takashi Sawada,Naoko Sugano,Marika Itabe,Hiroyuki Yamamoto,Matsuo
author_sort	Odanaka,Hibiki
title	Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins
title_short	Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins
title_full	Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins
title_fullStr	Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins
title_full_unstemmed	Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins
title_sort	comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100202
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_version_	1718441599509200896

Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins

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