The comprehensive detection of miRNA, lncRNA, and circRNA in regulation of mouse melanocyte and skin development

Abstract Background: Pigmentation development, is a complex process regulated by many transcription factors during development. With the development of the RNA sequencing (RNA-seq), non-coding RNAs, such as miRNAs, lncRNAs, and circRNAs, are found to play an important role in the function detection...

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Autores principales:	Zhu,Zhiwei, Ma,Yueyue, Li,Yuan, Li,Pengfei, Cheng,Zhixue, Li,Huifeng, Zhang,Lihuan, Tang,Zhongwei
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	miRNA lncRNA circRNA Skin Melanocyte Development
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100203
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spelling	oai:scielo:S0716-976020200001002032020-06-25The comprehensive detection of miRNA, lncRNA, and circRNA in regulation of mouse melanocyte and skin developmentZhu,ZhiweiMa,YueyueLi,YuanLi,PengfeiCheng,ZhixueLi,HuifengZhang,LihuanTang,Zhongwei miRNA lncRNA circRNA Skin Melanocyte Development Abstract Background: Pigmentation development, is a complex process regulated by many transcription factors during development. With the development of the RNA sequencing (RNA-seq), non-coding RNAs, such as miRNAs, lncRNAs, and circRNAs, are found to play an important role in the function detection of related regulation factors. In this study, we provided the expression profiles and development of ncRNAs related to melanocyte and skin development in mice with black coat color skin and mice with white coat color skin during embryonic day 15 (E15) and postnatal day 7 (P7). The expression profiles of different ncRNAs were detected via RNA-seq and also confirmed by the quantitative real-time PCR (qRT-PCR) method. GO and KEGG used to analyze the function the related target genes. Results: We identified an extensive catalogue of 206 and 183 differently expressed miRNAs, 600 and 800 differently expressed lncRNAs, and 50 and 54 differently expressed circRNAs, respectively. GO terms and pathway analysis showed the target genes of differentially expressed miRNA and lncRNA. The host genes of circRNA were mainly enriched in cellular process, single organism process. The target genes of miRNAs were mainly enriched in chromatin binding and calcium ion binding in the nucleus. The function of genes related to lncRNAs are post translation modification. The competing endogenous RNA (ceRNA) network of lncRNAs and circRNAs displays a complex interaction between ncRNA and mRNA related to skin development, such as Tcf4 , Gnas , and Gpnms related to melanocyte development. Conclusions: The ceRNA network of lncRNA and circRNA displays a complex interaction between ncRNA and mRNA related to skin development and melanocyte development. The embryonic and postnatal development of skin provide a reference for further studies on the development mechanisms of ncRNA during pigmentation.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100203en10.1186/s40659-020-0272-1
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	miRNA lncRNA circRNA Skin Melanocyte Development
spellingShingle	miRNA lncRNA circRNA Skin Melanocyte Development Zhu,Zhiwei Ma,Yueyue Li,Yuan Li,Pengfei Cheng,Zhixue Li,Huifeng Zhang,Lihuan Tang,Zhongwei The comprehensive detection of miRNA, lncRNA, and circRNA in regulation of mouse melanocyte and skin development
description	Abstract Background: Pigmentation development, is a complex process regulated by many transcription factors during development. With the development of the RNA sequencing (RNA-seq), non-coding RNAs, such as miRNAs, lncRNAs, and circRNAs, are found to play an important role in the function detection of related regulation factors. In this study, we provided the expression profiles and development of ncRNAs related to melanocyte and skin development in mice with black coat color skin and mice with white coat color skin during embryonic day 15 (E15) and postnatal day 7 (P7). The expression profiles of different ncRNAs were detected via RNA-seq and also confirmed by the quantitative real-time PCR (qRT-PCR) method. GO and KEGG used to analyze the function the related target genes. Results: We identified an extensive catalogue of 206 and 183 differently expressed miRNAs, 600 and 800 differently expressed lncRNAs, and 50 and 54 differently expressed circRNAs, respectively. GO terms and pathway analysis showed the target genes of differentially expressed miRNA and lncRNA. The host genes of circRNA were mainly enriched in cellular process, single organism process. The target genes of miRNAs were mainly enriched in chromatin binding and calcium ion binding in the nucleus. The function of genes related to lncRNAs are post translation modification. The competing endogenous RNA (ceRNA) network of lncRNAs and circRNAs displays a complex interaction between ncRNA and mRNA related to skin development, such as Tcf4 , Gnas , and Gpnms related to melanocyte development. Conclusions: The ceRNA network of lncRNA and circRNA displays a complex interaction between ncRNA and mRNA related to skin development and melanocyte development. The embryonic and postnatal development of skin provide a reference for further studies on the development mechanisms of ncRNA during pigmentation.
author	Zhu,Zhiwei Ma,Yueyue Li,Yuan Li,Pengfei Cheng,Zhixue Li,Huifeng Zhang,Lihuan Tang,Zhongwei
author_facet	Zhu,Zhiwei Ma,Yueyue Li,Yuan Li,Pengfei Cheng,Zhixue Li,Huifeng Zhang,Lihuan Tang,Zhongwei
author_sort	Zhu,Zhiwei
title	The comprehensive detection of miRNA, lncRNA, and circRNA in regulation of mouse melanocyte and skin development
title_short	The comprehensive detection of miRNA, lncRNA, and circRNA in regulation of mouse melanocyte and skin development
title_full	The comprehensive detection of miRNA, lncRNA, and circRNA in regulation of mouse melanocyte and skin development
title_fullStr	The comprehensive detection of miRNA, lncRNA, and circRNA in regulation of mouse melanocyte and skin development
title_full_unstemmed	The comprehensive detection of miRNA, lncRNA, and circRNA in regulation of mouse melanocyte and skin development
title_sort	comprehensive detection of mirna, lncrna, and circrna in regulation of mouse melanocyte and skin development
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100203
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The comprehensive detection of miRNA, lncRNA, and circRNA in regulation of mouse melanocyte and skin development

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