Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene

Abstract Background: The aim of this study was to investigate the effect role and mechanism of miR-30b-3p on ovarian cancer cells biological function. Methods: The expression of miR-30b-3p was detected in ovarian cancer cell lines and normal ovarian epithelial cell line by qRT-PCR. Mir-30b-3p mimi...

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Autores principales:	Li,Yan, Zhou,Jinhua, Wang,Juan, Chen,Xiaoping, Zhu,Yan, Chen,Youguo
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	miR-30b-3p Ovarian cancer OVCAR3 CTHRC1 EMT
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100208
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spelling	oai:scielo:S0716-976020200001002082020-06-25Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 geneLi,YanZhou,JinhuaWang,JuanChen,XiaopingZhu,YanChen,Youguo miR-30b-3p Ovarian cancer OVCAR3 CTHRC1 EMT Abstract Background: The aim of this study was to investigate the effect role and mechanism of miR-30b-3p on ovarian cancer cells biological function. Methods: The expression of miR-30b-3p was detected in ovarian cancer cell lines and normal ovarian epithelial cell line by qRT-PCR. Mir-30b-3p mimic was transfected into OVCAR3 cells. Cell-counting kit-8 (CCK-8) assay was conducted to explore the effect of mir-30b-3p on the OVCAR3 cells’ proliferation. Cell cycle and apoptosis were detected by Flow cytometry. Cell invasion ability was detected by Transwell test. The regulation of putative target of miR-30b-3p was verified by double luciferase reporter assays and Western blot. Result: We found that miR-30b-3p was downregulated in OVCAR3 cells. Overexpression of miR-30b-3p suppressed proliferation, promoted apoptosis, slowed cell cycle and inhibited migration and invasion of OVCAR3 cells. Bioinformatics analysis identified 3′-untranslated region (3′UTR) of Collagen triple helix repeat-containing 1 (CTHRC1) as the presumed binding site for miR-30b-3p. Detection of double luciferase reporter and Western-Blot result confirmed that CTHRC1 was the target gene of miR-30b-3p. Furthermore, E-cadherin, β-cadherin and Vimentin protein expression level were changed after transfection of miR-30b-3p. Conclusion: miR-30b-3p function as an anti-cancer gene. Overexpression of miR-30b-3p can inhibit the biological function of ovarian cancer cells. MiR-30b-3p targets CTHRC1 gene plays an important role in epithelial–mesenchymal transformation (EMT), and supports miR-30b-3p as a potential biological indicator for ovarian cancer in the future.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100208en10.1186/s40659-020-00277-4
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	miR-30b-3p Ovarian cancer OVCAR3 CTHRC1 EMT
spellingShingle	miR-30b-3p Ovarian cancer OVCAR3 CTHRC1 EMT Li,Yan Zhou,Jinhua Wang,Juan Chen,Xiaoping Zhu,Yan Chen,Youguo Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene
description	Abstract Background: The aim of this study was to investigate the effect role and mechanism of miR-30b-3p on ovarian cancer cells biological function. Methods: The expression of miR-30b-3p was detected in ovarian cancer cell lines and normal ovarian epithelial cell line by qRT-PCR. Mir-30b-3p mimic was transfected into OVCAR3 cells. Cell-counting kit-8 (CCK-8) assay was conducted to explore the effect of mir-30b-3p on the OVCAR3 cells’ proliferation. Cell cycle and apoptosis were detected by Flow cytometry. Cell invasion ability was detected by Transwell test. The regulation of putative target of miR-30b-3p was verified by double luciferase reporter assays and Western blot. Result: We found that miR-30b-3p was downregulated in OVCAR3 cells. Overexpression of miR-30b-3p suppressed proliferation, promoted apoptosis, slowed cell cycle and inhibited migration and invasion of OVCAR3 cells. Bioinformatics analysis identified 3′-untranslated region (3′UTR) of Collagen triple helix repeat-containing 1 (CTHRC1) as the presumed binding site for miR-30b-3p. Detection of double luciferase reporter and Western-Blot result confirmed that CTHRC1 was the target gene of miR-30b-3p. Furthermore, E-cadherin, β-cadherin and Vimentin protein expression level were changed after transfection of miR-30b-3p. Conclusion: miR-30b-3p function as an anti-cancer gene. Overexpression of miR-30b-3p can inhibit the biological function of ovarian cancer cells. MiR-30b-3p targets CTHRC1 gene plays an important role in epithelial–mesenchymal transformation (EMT), and supports miR-30b-3p as a potential biological indicator for ovarian cancer in the future.
author	Li,Yan Zhou,Jinhua Wang,Juan Chen,Xiaoping Zhu,Yan Chen,Youguo
author_facet	Li,Yan Zhou,Jinhua Wang,Juan Chen,Xiaoping Zhu,Yan Chen,Youguo
author_sort	Li,Yan
title	Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene
title_short	Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene
title_full	Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene
title_fullStr	Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene
title_full_unstemmed	Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene
title_sort	mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the cthrc1 gene
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100208
work_keys_str_mv	AT liyan mir30b3paffectsthemigrationandinvasionfunctionofovariancancercellsbytargetingthecthrc1gene AT zhoujinhua mir30b3paffectsthemigrationandinvasionfunctionofovariancancercellsbytargetingthecthrc1gene AT wangjuan mir30b3paffectsthemigrationandinvasionfunctionofovariancancercellsbytargetingthecthrc1gene AT chenxiaoping mir30b3paffectsthemigrationandinvasionfunctionofovariancancercellsbytargetingthecthrc1gene AT zhuyan mir30b3paffectsthemigrationandinvasionfunctionofovariancancercellsbytargetingthecthrc1gene AT chenyouguo mir30b3paffectsthemigrationandinvasionfunctionofovariancancercellsbytargetingthecthrc1gene
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Mir-30b-3p affects the migration and invasion function of ovarian cancer cells by targeting the CTHRC1 gene

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