Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor

Abstract Background: Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We descr...

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Autores principales:	García,Patricia, Bizama,Carolina, Rosa,Lorena, Espinoza,Jaime A., Weber,Helga, Cerda-Infante,Javier, Sánchez,Marianela, Montecinos,Viviana P., Lorenzo-Bermejo,Justo, Boekstegers,Felix, Dávila-López,Marcela, Alfaro,Francisca, Leiva-Acevedo,Claudia, Parra,Zasha, Romero,Diego, Kato,Sumie, Leal,Pamela, Lagos,Marcela, Roa,Juan Carlos
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	Gallbladder cancer Cancer cell lines Ascites Native American ancestry Gene expression profile
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100211
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spelling	oai:scielo:S0716-976020200001002112020-06-25Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumorGarcía,PatriciaBizama,CarolinaRosa,LorenaEspinoza,Jaime A.Weber,HelgaCerda-Infante,JavierSánchez,MarianelaMontecinos,Viviana P.Lorenzo-Bermejo,JustoBoekstegers,FelixDávila-López,MarcelaAlfaro,FranciscaLeiva-Acevedo,ClaudiaParra,ZashaRomero,DiegoKato,SumieLeal,PamelaLagos,MarcelaRoa,Juan Carlos Gallbladder cancer Cancer cell lines Ascites Native American ancestry Gene expression profile Abstract Background: Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry. Results: After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice. Conclusions: The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100211en10.1186/s40659-020-00282-7
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gallbladder cancer Cancer cell lines Ascites Native American ancestry Gene expression profile
spellingShingle	Gallbladder cancer Cancer cell lines Ascites Native American ancestry Gene expression profile García,Patricia Bizama,Carolina Rosa,Lorena Espinoza,Jaime A. Weber,Helga Cerda-Infante,Javier Sánchez,Marianela Montecinos,Viviana P. Lorenzo-Bermejo,Justo Boekstegers,Felix Dávila-López,Marcela Alfaro,Francisca Leiva-Acevedo,Claudia Parra,Zasha Romero,Diego Kato,Sumie Leal,Pamela Lagos,Marcela Roa,Juan Carlos Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
description	Abstract Background: Gallbladder cancer (GBC) is the most common tumor of the biliary tract. The incidence of GBC shows a large geographic variability, being particularly frequent in Native American populations. In Chile, GBC represents the second cause of cancer-related death among women. We describe here the establishment of three novel cell lines derived from the ascitic fluid of a Chilean GBC patient, who presented 46% European, 36% Mapuche, 12% Aymara and 6% African ancestry. Results: After immunocytochemical staining of the primary cell culture, we isolated and comprehensively characterized three independent clones (PUC-GBC1, PUC-GBC2 and PUC-GBC3) by short tandem repeat DNA profiling and RNA sequencing as well as karyotype, doubling time, chemosensitivity, in vitro migration capability and in vivo tumorigenicity assay. Primary culture cells showed high expression of CK7, CK19, CA 19-9, MUC1 and MUC16, and negative expression of mesothelial markers. The three isolated clones displayed an epithelial phenotype and an abnormal structure and number of chromosomes. RNA sequencing confirmed the increased expression of cytokeratin and mucin genes, and also of TP53 and ERBB2 with some differences among the three cells lines, and revealed a novel exonic mutation in NF1. The PUC-GBC3 clone was the most aggressive according to histopathological features and the tumorigenic capacity in NSG mice. Conclusions: The first cell lines established from a Chilean GBC patient represent a new model for studying GBC in patients of Native American descent.
author	García,Patricia Bizama,Carolina Rosa,Lorena Espinoza,Jaime A. Weber,Helga Cerda-Infante,Javier Sánchez,Marianela Montecinos,Viviana P. Lorenzo-Bermejo,Justo Boekstegers,Felix Dávila-López,Marcela Alfaro,Francisca Leiva-Acevedo,Claudia Parra,Zasha Romero,Diego Kato,Sumie Leal,Pamela Lagos,Marcela Roa,Juan Carlos
author_facet	García,Patricia Bizama,Carolina Rosa,Lorena Espinoza,Jaime A. Weber,Helga Cerda-Infante,Javier Sánchez,Marianela Montecinos,Viviana P. Lorenzo-Bermejo,Justo Boekstegers,Felix Dávila-López,Marcela Alfaro,Francisca Leiva-Acevedo,Claudia Parra,Zasha Romero,Diego Kato,Sumie Leal,Pamela Lagos,Marcela Roa,Juan Carlos
author_sort	García,Patricia
title	Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
title_short	Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
title_full	Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
title_fullStr	Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
title_full_unstemmed	Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
title_sort	functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100211
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Functional and genomic characterization of three novel cell lines derived from a metastatic gallbladder cancer tumor

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