LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p

Abstract Background: Previous studies have shown that long noncoding RNA (IncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this IncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the eff...

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Autores principales:	Luo,Min, Liang,Chengbai
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	Gastric cancer LINC00483 miR-490-3p MAPK1
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100212
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spelling	oai:scielo:S0716-976020200001002122020-06-25LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3pLuo,MinLiang,Chengbai Gastric cancer LINC00483 miR-490-3p MAPK1 Abstract Background: Previous studies have shown that long noncoding RNA (IncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this IncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the effect of LINC00483 on gastric cancer development and explore the potential regulatory network of LINC00483/microRNA (miR)-490-3p/mitogen-activated protein kinase 1 (MAPK1). Methods: Thirty patients with gastric cancer were recruited for tissues collection. The expression levels of LINC00483, miR-490-3p and MAPK1 were detected by quantitative real-time polymerase chain reaction or western blot. Cell viability, apoptosis, migration and invasion were determined by MTT, flow cytometry, transwell assays and western blot, respectively. The target association between miR-490-3p and LINC00483 or MAPK1 was confirmed by luciferase reporter assay. Xenograft model was established to assess the function of LINC00483 in vivo. Results: LINC00483 and MAPK1 levels were increased in gastric cancer tissues and cells. Knockdown of LINC00483 or MAPK1 inhibited cells viability, migration and invasion but promoted apoptosis in gastric cancer cells. Moreover, MAPK1 overexpression attenuated the effect of LINC00483 knockdown on gastric cancer development. LINC00483 could increase MAPK1 expression by competitively sponging miR-490-3p. miR-490-3p overexpression suppressed gastric cancer development, which was abated by introduction of LINC00483. Besides, inhibition of LINC00483 decreased xenograft tumor growth by regulating miR-490-3p/MAPK1 axis. Conclusion: Knockdown of LINC00483 inhibited gastric cancer development in vitro and in vivo by increasing miR- 490-3p and decreasing MAPK1, elucidating a novel mechanism for understanding the development of gastric cancer.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100212en10.1186/s40659-020-00283-6
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gastric cancer LINC00483 miR-490-3p MAPK1
spellingShingle	Gastric cancer LINC00483 miR-490-3p MAPK1 Luo,Min Liang,Chengbai LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p
description	Abstract Background: Previous studies have shown that long noncoding RNA (IncRNA) LINC00483 was aberrantly expressed in human cancers, including gastric cancer. However, the regulatory mechanism of this IncRNA in gastric cancer remains largely unknown. The present study aimed to investigate the effect of LINC00483 on gastric cancer development and explore the potential regulatory network of LINC00483/microRNA (miR)-490-3p/mitogen-activated protein kinase 1 (MAPK1). Methods: Thirty patients with gastric cancer were recruited for tissues collection. The expression levels of LINC00483, miR-490-3p and MAPK1 were detected by quantitative real-time polymerase chain reaction or western blot. Cell viability, apoptosis, migration and invasion were determined by MTT, flow cytometry, transwell assays and western blot, respectively. The target association between miR-490-3p and LINC00483 or MAPK1 was confirmed by luciferase reporter assay. Xenograft model was established to assess the function of LINC00483 in vivo. Results: LINC00483 and MAPK1 levels were increased in gastric cancer tissues and cells. Knockdown of LINC00483 or MAPK1 inhibited cells viability, migration and invasion but promoted apoptosis in gastric cancer cells. Moreover, MAPK1 overexpression attenuated the effect of LINC00483 knockdown on gastric cancer development. LINC00483 could increase MAPK1 expression by competitively sponging miR-490-3p. miR-490-3p overexpression suppressed gastric cancer development, which was abated by introduction of LINC00483. Besides, inhibition of LINC00483 decreased xenograft tumor growth by regulating miR-490-3p/MAPK1 axis. Conclusion: Knockdown of LINC00483 inhibited gastric cancer development in vitro and in vivo by increasing miR- 490-3p and decreasing MAPK1, elucidating a novel mechanism for understanding the development of gastric cancer.
author	Luo,Min Liang,Chengbai
author_facet	Luo,Min Liang,Chengbai
author_sort	Luo,Min
title	LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p
title_short	LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p
title_full	LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p
title_fullStr	LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p
title_full_unstemmed	LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p
title_sort	lncrna linc00483 promotes gastric cancer development through regulating mapk1 expression by sponging mir-490-3p
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100212
work_keys_str_mv	AT luomin lncrnalinc00483promotesgastriccancerdevelopmentthroughregulatingmapk1expressionbyspongingmir4903p AT liangchengbai lncrnalinc00483promotesgastriccancerdevelopmentthroughregulatingmapk1expressionbyspongingmir4903p
_version_	1718441602400124928

LncRNA LINC00483 promotes gastric cancer development through regulating MAPK1 expression by sponging miR-490-3p

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