miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2

Abstract Background: Breast cancer is the most common cancer types among women. Recent researches have focused on determining the efficiency of alternative molecules and miRNAs in breast cancer treatment. The aim of this study was to determine the effect of usnic acid response-miR-185-5p on prolife...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Değerli,Elif, Torun,Vildan, Cansaran-Duman,Demet
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2020
Materias:
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100214
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:scielo:S0716-97602020000100214
record_format dspace
spelling oai:scielo:S0716-976020200001002142020-06-25miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2Değerli,ElifTorun,VildanCansaran-Duman,Demet miR-185-5p Usnic acid Breast cancer Apoptosis BCL2 Abstract Background: Breast cancer is the most common cancer types among women. Recent researches have focused on determining the efficiency of alternative molecules and miRNAs in breast cancer treatment. The aim of this study was to determine the effect of usnic acid response-miR-185-5p on proliferation in the breast cancer cell and to determine its relationship with apoptosis pathway. Methods: The cell proliferation and cell apoptosis rate were significantly increased following the ectopic expression of miR-185-5p in BT-474 cells. Furthermore, the results of cell cycle assay performed by flow cytometry revealed that the transfection with miR-185-5p induced G1/S phase arrest. The apoptosis-related genes expression analysis was performed by qRT-PCR and the direct target of miR-185-5p in BT-474 cells was identified by western blot and luciferase reporter assay. Results: Our data showed that miR-185-5p can cause significant changes in apoptosis-related genes expression levels, suggesting that cell proliferation was suppressed by miR-185-5p via inducing apoptosis in breast cancer cells. According to western blot results, miR-185-5p lead to decrease BCL2 protein level in BT-474 cells and direct target of miR-185-5p was identified as BCL by luciferase reporter assay. Conclusion: This study revealed that miR-185-5p may be an effective agent in the treatment of breast cancer.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100214en10.1186/s40659-020-00285-4
institution Scielo Chile
collection Scielo Chile
language English
topic miR-185-5p
Usnic acid
Breast cancer
Apoptosis
BCL2
spellingShingle miR-185-5p
Usnic acid
Breast cancer
Apoptosis
BCL2
Değerli,Elif
Torun,Vildan
Cansaran-Duman,Demet
miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2
description Abstract Background: Breast cancer is the most common cancer types among women. Recent researches have focused on determining the efficiency of alternative molecules and miRNAs in breast cancer treatment. The aim of this study was to determine the effect of usnic acid response-miR-185-5p on proliferation in the breast cancer cell and to determine its relationship with apoptosis pathway. Methods: The cell proliferation and cell apoptosis rate were significantly increased following the ectopic expression of miR-185-5p in BT-474 cells. Furthermore, the results of cell cycle assay performed by flow cytometry revealed that the transfection with miR-185-5p induced G1/S phase arrest. The apoptosis-related genes expression analysis was performed by qRT-PCR and the direct target of miR-185-5p in BT-474 cells was identified by western blot and luciferase reporter assay. Results: Our data showed that miR-185-5p can cause significant changes in apoptosis-related genes expression levels, suggesting that cell proliferation was suppressed by miR-185-5p via inducing apoptosis in breast cancer cells. According to western blot results, miR-185-5p lead to decrease BCL2 protein level in BT-474 cells and direct target of miR-185-5p was identified as BCL by luciferase reporter assay. Conclusion: This study revealed that miR-185-5p may be an effective agent in the treatment of breast cancer.
author Değerli,Elif
Torun,Vildan
Cansaran-Duman,Demet
author_facet Değerli,Elif
Torun,Vildan
Cansaran-Duman,Demet
author_sort Değerli,Elif
title miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2
title_short miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2
title_full miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2
title_fullStr miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2
title_full_unstemmed miR-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting Bcl2
title_sort mir-185-5p response to usnic acid suppresses proliferation and regulating apoptosis in breast cancer cell by targeting bcl2
publisher Sociedad de Biología de Chile
publishDate 2020
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100214
work_keys_str_mv AT de287erlielif mir1855presponsetousnicacidsuppressesproliferationandregulatingapoptosisinbreastcancercellbytargetingbcl2
AT torunvildan mir1855presponsetousnicacidsuppressesproliferationandregulatingapoptosisinbreastcancercellbytargetingbcl2
AT cansarandumandemet mir1855presponsetousnicacidsuppressesproliferationandregulatingapoptosisinbreastcancercellbytargetingbcl2
_version_ 1718441603096379392