Understanding gold toxicity in aerobically-grown Escherichia coli

Abstract Background: There Is an emerging field to put Into practice new strategies for developing molecules with antimicrobial properties. In this line, several metals and metalloids are currently being used for these purposes, although their cellular effect(s) or target(s) in a particular organis...

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Autores principales:	Muñoz-Villagrán,C., Contreras,F., Cornejo,F., Figueroa,M., Valenzuela-Bezanilla,D., Luraschi,R., Reinoso,C., Rivas-Pardo,J., Vásquez,C., Castro,M., Arenas,F.
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	Gold(III) Toxicity Resistance E. coli Aerobic Anaerobic
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100222
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spelling	oai:scielo:S0716-976020200001002222020-07-02Understanding gold toxicity in aerobically-grown Escherichia coliMuñoz-Villagrán,C.Contreras,F.Cornejo,F.Figueroa,M.Valenzuela-Bezanilla,D.Luraschi,R.Reinoso,C.Rivas-Pardo,J.Vásquez,C.Castro,M.Arenas,F. Gold(III) Toxicity Resistance E. coli Aerobic Anaerobic Abstract Background: There Is an emerging field to put Into practice new strategies for developing molecules with antimicrobial properties. In this line, several metals and metalloids are currently being used for these purposes, although their cellular effect(s) or target(s) in a particular organism are still unknown. Here we aimed to investigate and analyze Au3+ toxicity through a combination of biochemical and molecular approaches. Results: We found that Au3+ triggers a major oxidative unbalance in Escherichia coli, characterized by decreased intracellular thiol levels, increased superoxide concentration, as well as by an augmented production of the antioxidant enzymes superoxide dismutase and catalase. Because ROS production is, in some cases, associated with metal reduction and the concomitant generation of gold-containing nanostructures (AuNS), this possibility was evaluated in vivo and in vitro. Conclusions: Au3+ is toxic for E. coli because it triggers an unbalance of the bacterium's oxidative status. This was demonstrated by using oxidative stress dyes and antioxidant chemicals as well as gene reporters, RSH concentrations and AuNS generation.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100222en10.1186/s40659-020-00292-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gold(III) Toxicity Resistance E. coli Aerobic Anaerobic
spellingShingle	Gold(III) Toxicity Resistance E. coli Aerobic Anaerobic Muñoz-Villagrán,C. Contreras,F. Cornejo,F. Figueroa,M. Valenzuela-Bezanilla,D. Luraschi,R. Reinoso,C. Rivas-Pardo,J. Vásquez,C. Castro,M. Arenas,F. Understanding gold toxicity in aerobically-grown Escherichia coli
description	Abstract Background: There Is an emerging field to put Into practice new strategies for developing molecules with antimicrobial properties. In this line, several metals and metalloids are currently being used for these purposes, although their cellular effect(s) or target(s) in a particular organism are still unknown. Here we aimed to investigate and analyze Au3+ toxicity through a combination of biochemical and molecular approaches. Results: We found that Au3+ triggers a major oxidative unbalance in Escherichia coli, characterized by decreased intracellular thiol levels, increased superoxide concentration, as well as by an augmented production of the antioxidant enzymes superoxide dismutase and catalase. Because ROS production is, in some cases, associated with metal reduction and the concomitant generation of gold-containing nanostructures (AuNS), this possibility was evaluated in vivo and in vitro. Conclusions: Au3+ is toxic for E. coli because it triggers an unbalance of the bacterium's oxidative status. This was demonstrated by using oxidative stress dyes and antioxidant chemicals as well as gene reporters, RSH concentrations and AuNS generation.
author	Muñoz-Villagrán,C. Contreras,F. Cornejo,F. Figueroa,M. Valenzuela-Bezanilla,D. Luraschi,R. Reinoso,C. Rivas-Pardo,J. Vásquez,C. Castro,M. Arenas,F.
author_facet	Muñoz-Villagrán,C. Contreras,F. Cornejo,F. Figueroa,M. Valenzuela-Bezanilla,D. Luraschi,R. Reinoso,C. Rivas-Pardo,J. Vásquez,C. Castro,M. Arenas,F.
author_sort	Muñoz-Villagrán,C.
title	Understanding gold toxicity in aerobically-grown Escherichia coli
title_short	Understanding gold toxicity in aerobically-grown Escherichia coli
title_full	Understanding gold toxicity in aerobically-grown Escherichia coli
title_fullStr	Understanding gold toxicity in aerobically-grown Escherichia coli
title_full_unstemmed	Understanding gold toxicity in aerobically-grown Escherichia coli
title_sort	understanding gold toxicity in aerobically-grown escherichia coli
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100222
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Understanding gold toxicity in aerobically-grown Escherichia coli

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