Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' disease

Abstract Background: Circulating RNA (circRNA) regulates various bioactivities in cells. A better understanding of the exosomal circRNA can provide novel insights into the pathogenesis and treatment of Graves' disease (GD). We aimed to profile the differentially expressed circRNAs (DEcRs) in p...

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Autores principales:	Sun,Ying, Wang,Wei, Tang,Yuxiao, Wang,Daping, Li,Liang, Na,Min, Jiang,Guantong, Li,Qian, Chen,Shulin, Zhou,Jin
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	Circular RNA Graves' disease Exosomes hsa_circRNA_000102 Immune activation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100226
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spelling	oai:scielo:S0716-976020200001002262020-08-07Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' diseaseSun,YingWang,WeiTang,YuxiaoWang,DapingLi,LiangNa,MinJiang,GuantongLi,QianChen,ShulinZhou,Jin Circular RNA Graves' disease Exosomes hsa_circRNA_000102 Immune activation Abstract Background: Circulating RNA (circRNA) regulates various bioactivities in cells. A better understanding of the exosomal circRNA can provide novel insights into the pathogenesis and treatment of Graves' disease (GD). We aimed to profile the differentially expressed circRNAs (DEcRs) in plasma exosomes of patients with GD and speculate and probe the functions of the DEcR by comprehensive bioinformatics analyses. Methods: Serum exosomes were isolated from five primary GD patients and five healthy controls via ultracentrifugation. After verification with transmission electron microscopy, exosome samples were subjected to microarray profiling using human circRNA microarrays. Two up-regulated and two down-regulated DEcRs were selected for validation in plasma exosomes from 20 GD and 20 healthy control participants using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The circRNA/microRNA/mRNA interaction network was then assembled and the analysis of the Gene Ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways was utilized to predict the potential functions of the DEcR associated genes. Results: There were 15 DEcRs revealed in primary GD cases. The intronic circRNA hsa_circRNA_000102 was confirmed as an up-regulated component in plasma exosomes from patients with GD. The circRNA/microRNA/mRNA interaction network unveiled the most potential targeting microRNAs of hsa_circRNA_000102 and its associated genes. The functional analyses predicted involvement of hsa_circRNA_000102 associated genes in pathways of immune system activation, such as viral infection and interferon-beta signaling. Conclusions: hsa_circRNA_000102 is a differentially up-regulated plasma exosomal circRNA in patients with GD. Our study highlights multiple pathways, particularly virus infection and interferon-beta signaling, for mediating immune activation in Graves' disease.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100226en10.1186/s40659-020-00299-y
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Circular RNA Graves' disease Exosomes hsa_circRNA_000102 Immune activation
spellingShingle	Circular RNA Graves' disease Exosomes hsa_circRNA_000102 Immune activation Sun,Ying Wang,Wei Tang,Yuxiao Wang,Daping Li,Liang Na,Min Jiang,Guantong Li,Qian Chen,Shulin Zhou,Jin Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' disease
description	Abstract Background: Circulating RNA (circRNA) regulates various bioactivities in cells. A better understanding of the exosomal circRNA can provide novel insights into the pathogenesis and treatment of Graves' disease (GD). We aimed to profile the differentially expressed circRNAs (DEcRs) in plasma exosomes of patients with GD and speculate and probe the functions of the DEcR by comprehensive bioinformatics analyses. Methods: Serum exosomes were isolated from five primary GD patients and five healthy controls via ultracentrifugation. After verification with transmission electron microscopy, exosome samples were subjected to microarray profiling using human circRNA microarrays. Two up-regulated and two down-regulated DEcRs were selected for validation in plasma exosomes from 20 GD and 20 healthy control participants using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR). The circRNA/microRNA/mRNA interaction network was then assembled and the analysis of the Gene Ontology and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways was utilized to predict the potential functions of the DEcR associated genes. Results: There were 15 DEcRs revealed in primary GD cases. The intronic circRNA hsa_circRNA_000102 was confirmed as an up-regulated component in plasma exosomes from patients with GD. The circRNA/microRNA/mRNA interaction network unveiled the most potential targeting microRNAs of hsa_circRNA_000102 and its associated genes. The functional analyses predicted involvement of hsa_circRNA_000102 associated genes in pathways of immune system activation, such as viral infection and interferon-beta signaling. Conclusions: hsa_circRNA_000102 is a differentially up-regulated plasma exosomal circRNA in patients with GD. Our study highlights multiple pathways, particularly virus infection and interferon-beta signaling, for mediating immune activation in Graves' disease.
author	Sun,Ying Wang,Wei Tang,Yuxiao Wang,Daping Li,Liang Na,Min Jiang,Guantong Li,Qian Chen,Shulin Zhou,Jin
author_facet	Sun,Ying Wang,Wei Tang,Yuxiao Wang,Daping Li,Liang Na,Min Jiang,Guantong Li,Qian Chen,Shulin Zhou,Jin
author_sort	Sun,Ying
title	Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' disease
title_short	Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' disease
title_full	Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' disease
title_fullStr	Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' disease
title_full_unstemmed	Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' disease
title_sort	microarray profiling and functional analysis of differentially expressed plasma exosomal circular rnas in graves' disease
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100226
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Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves' disease

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