Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723

Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723

Abstract Background: Spinal cord injury (SCI) is a severe central nervous system trauma. The present study aimed to evaluate the effect of HIF-1α on inflammation in spinal cord injury (SCI) to uncover the molecular mechanisms of anti-inflammation. Results: HIF-1α was reduced in S...

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Autores principales: Li,Xigong, Lou,Xianfeng, Xu,Sanzhong, Du,Junhua, Wu,Junsong
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2020
Materias:
HIF-1α
Circ 0001723
miR-380-3p
NLRP3
Spinal cord injury
Inflammation
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100227
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spelling oai:scielo:S0716-976020200001002272020-09-27Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723Li,XigongLou,XianfengXu,SanzhongDu,JunhuaWu,Junsong HIF-1α Circ 0001723 miR-380-3p NLRP3 Spinal cord injury Inflammation Abstract Background: Spinal cord injury (SCI) is a severe central nervous system trauma. The present study aimed to evaluate the effect of HIF-1α on inflammation in spinal cord injury (SCI) to uncover the molecular mechanisms of anti-inflammation. Results: HIF-1α was reduced in SCI model rats and HIF-1α activation reduced TNF-α, IL-1β, IL-6 and IL-18 levels in SCI model rats. Meanwhile, Circ 0001723 expression was down-regulated and miR-380-3p expression was up-regulated in SCI model rats. In vitro model, down-regulation of Circ 0001723 promoted TNF-α, IL-1β, IL-6 and IL-18 levels, compared with control negative group. However, over-expression of Circ 0001723 reduced TNF-α, IL-1β, IL-6 and IL-18 levels in vitro model. Down-regulation of Circ 0001723 suppressed HIF-1α protein expressions and induced NLRP3 and Caspase-1 protein expressions in vitro model by up-regulation of miR-380-3p. Next, inactivation of HIF-1α reduced the pro-inflammation effects of Circ 0001723 in vitro model. Then, si-NLRP3 also inhibited the pro-inflammation effects of Circ 0001723 in vitro model via promotion of autophagy. Conclusions: We concluded that HIF-Ια reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100227en10.1186/s40659-020-00302-6
institution Scielo Chile
collection Scielo Chile
language English
topic HIF-1α
Circ 0001723
miR-380-3p
NLRP3
Spinal cord injury
Inflammation
spellingShingle HIF-1α
Circ 0001723
miR-380-3p
NLRP3
Spinal cord injury
Inflammation
Li,Xigong
Lou,Xianfeng
Xu,Sanzhong
Du,Junhua
Wu,Junsong
Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723
description Abstract Background: Spinal cord injury (SCI) is a severe central nervous system trauma. The present study aimed to evaluate the effect of HIF-1α on inflammation in spinal cord injury (SCI) to uncover the molecular mechanisms of anti-inflammation. Results: HIF-1α was reduced in SCI model rats and HIF-1α activation reduced TNF-α, IL-1β, IL-6 and IL-18 levels in SCI model rats. Meanwhile, Circ 0001723 expression was down-regulated and miR-380-3p expression was up-regulated in SCI model rats. In vitro model, down-regulation of Circ 0001723 promoted TNF-α, IL-1β, IL-6 and IL-18 levels, compared with control negative group. However, over-expression of Circ 0001723 reduced TNF-α, IL-1β, IL-6 and IL-18 levels in vitro model. Down-regulation of Circ 0001723 suppressed HIF-1α protein expressions and induced NLRP3 and Caspase-1 protein expressions in vitro model by up-regulation of miR-380-3p. Next, inactivation of HIF-1α reduced the pro-inflammation effects of Circ 0001723 in vitro model. Then, si-NLRP3 also inhibited the pro-inflammation effects of Circ 0001723 in vitro model via promotion of autophagy. Conclusions: We concluded that HIF-Ια reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723.
author Li,Xigong
Lou,Xianfeng
Xu,Sanzhong
Du,Junhua
Wu,Junsong
author_facet Li,Xigong
Lou,Xianfeng
Xu,Sanzhong
Du,Junhua
Wu,Junsong
author_sort Li,Xigong
title Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723
title_short Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723
title_full Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723
title_fullStr Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723
title_full_unstemmed Hypoxia inducible factor-1 (HIF-1α) reduced inflammation in spinal cord injury via miR-380-3p/ NLRP3 by Circ 0001723
title_sort hypoxia inducible factor-1 (hif-1α) reduced inflammation in spinal cord injury via mir-380-3p/ nlrp3 by circ 0001723
publisher Sociedad de Biología de Chile
publishDate 2020
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100227
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AT louxianfeng hypoxiainduciblefactor1hif1945reducedinflammationinspinalcordinjuryviamir3803pnlrp3bycirc0001723
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AT dujunhua hypoxiainduciblefactor1hif1945reducedinflammationinspinalcordinjuryviamir3803pnlrp3bycirc0001723
AT wujunsong hypoxiainduciblefactor1hif1945reducedinflammationinspinalcordinjuryviamir3803pnlrp3bycirc0001723
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