Palmatine ameliorates high fat diet induced impaired glucose tolerance

Abstract Background: The impaired glucose tolerance (IGT) is a representative prediabetes characterized by defective glucose homeostasis, and palmatine (PAL) is a natural isoquinoline alkaloid with multiple pharmacological effects. Our study aims to investigate the therapeutic effect of PAL on the...

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Autores principales:	Tian,Xusheng, Zhang,Yukun, Li,Han, Li,Yunfeng, Wang,Ning, Zhang,Wei, Ma,Boyan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	Palmatine Impaired glucose tolerance Insulin resistance ERK signaling
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100230
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spelling	oai:scielo:S0716-976020200001002302020-09-27Palmatine ameliorates high fat diet induced impaired glucose toleranceTian,XushengZhang,YukunLi,HanLi,YunfengWang,NingZhang,WeiMa,Boyan Palmatine Impaired glucose tolerance Insulin resistance ERK signaling Abstract Background: The impaired glucose tolerance (IGT) is a representative prediabetes characterized by defective glucose homeostasis, and palmatine (PAL) is a natural isoquinoline alkaloid with multiple pharmacological effects. Our study aims to investigate the therapeutic effect of PAL on the impaired glucose tolerance. Methods: Male Sprague-Dawley rats were used to establish an IGT model with high fat diet (HFD). Oral glucose tolerance test (OGTT) and further biochemical analysis were conducted to determine the effect of PAL on glucose intolerance in vivo. Molecular details were clarified in a cellular model of IGT induced by Palmitate (PA) on INS-1 cells. Results: Our study demonstrated a relief of IGT with improved insulin resistance in HFD induced rats after PAL treatment. Besides, promoted pancreas islets function was validated with significantly increased β cell mass after the treatment of PAL. We further found out that PAL could alleviate the β cell apoptosis that accounts for β cell mass loss in IGT model. Moreover, MAPK signaling was investigated in vivo and vitro with the discovery that PAL regulated the MAPK signaling by restricting the ERK and JNK cascades. The insulin secretion assay indicated that PAL significantly promoted the defective insulin secretion in PA-induced INS-1 cells via JNK rather than ERK signaling. Furthermore, PAL treatment was determined to significantly suppress β cell apoptosis in PA-induced cells. We thus thought that PAL promoted the PA-induced impaired insulin release by inhibiting the β cell apoptosis and JNK signaling in vitro. Conclusion: In summary, PAL ameliorates HFD-induced IGT with novel mechanisms.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100230en10.1186/s40659-020-00308-0
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Palmatine Impaired glucose tolerance Insulin resistance ERK signaling
spellingShingle	Palmatine Impaired glucose tolerance Insulin resistance ERK signaling Tian,Xusheng Zhang,Yukun Li,Han Li,Yunfeng Wang,Ning Zhang,Wei Ma,Boyan Palmatine ameliorates high fat diet induced impaired glucose tolerance
description	Abstract Background: The impaired glucose tolerance (IGT) is a representative prediabetes characterized by defective glucose homeostasis, and palmatine (PAL) is a natural isoquinoline alkaloid with multiple pharmacological effects. Our study aims to investigate the therapeutic effect of PAL on the impaired glucose tolerance. Methods: Male Sprague-Dawley rats were used to establish an IGT model with high fat diet (HFD). Oral glucose tolerance test (OGTT) and further biochemical analysis were conducted to determine the effect of PAL on glucose intolerance in vivo. Molecular details were clarified in a cellular model of IGT induced by Palmitate (PA) on INS-1 cells. Results: Our study demonstrated a relief of IGT with improved insulin resistance in HFD induced rats after PAL treatment. Besides, promoted pancreas islets function was validated with significantly increased β cell mass after the treatment of PAL. We further found out that PAL could alleviate the β cell apoptosis that accounts for β cell mass loss in IGT model. Moreover, MAPK signaling was investigated in vivo and vitro with the discovery that PAL regulated the MAPK signaling by restricting the ERK and JNK cascades. The insulin secretion assay indicated that PAL significantly promoted the defective insulin secretion in PA-induced INS-1 cells via JNK rather than ERK signaling. Furthermore, PAL treatment was determined to significantly suppress β cell apoptosis in PA-induced cells. We thus thought that PAL promoted the PA-induced impaired insulin release by inhibiting the β cell apoptosis and JNK signaling in vitro. Conclusion: In summary, PAL ameliorates HFD-induced IGT with novel mechanisms.
author	Tian,Xusheng Zhang,Yukun Li,Han Li,Yunfeng Wang,Ning Zhang,Wei Ma,Boyan
author_facet	Tian,Xusheng Zhang,Yukun Li,Han Li,Yunfeng Wang,Ning Zhang,Wei Ma,Boyan
author_sort	Tian,Xusheng
title	Palmatine ameliorates high fat diet induced impaired glucose tolerance
title_short	Palmatine ameliorates high fat diet induced impaired glucose tolerance
title_full	Palmatine ameliorates high fat diet induced impaired glucose tolerance
title_fullStr	Palmatine ameliorates high fat diet induced impaired glucose tolerance
title_full_unstemmed	Palmatine ameliorates high fat diet induced impaired glucose tolerance
title_sort	palmatine ameliorates high fat diet induced impaired glucose tolerance
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100230
work_keys_str_mv	AT tianxusheng palmatineameliorateshighfatdietinducedimpairedglucosetolerance AT zhangyukun palmatineameliorateshighfatdietinducedimpairedglucosetolerance AT lihan palmatineameliorateshighfatdietinducedimpairedglucosetolerance AT liyunfeng palmatineameliorateshighfatdietinducedimpairedglucosetolerance AT wangning palmatineameliorateshighfatdietinducedimpairedglucosetolerance AT zhangwei palmatineameliorateshighfatdietinducedimpairedglucosetolerance AT maboyan palmatineameliorateshighfatdietinducedimpairedglucosetolerance
_version_	1718441607545487360

Palmatine ameliorates high fat diet induced impaired glucose tolerance

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