Tumor suppressive function of Matrin 3 in the basal-like breast cancer

Abstract Background: Basal-like breast cancer (BLBC) or triple-negative breast cancer (TNBC) is an aggressive and highly metastatic subtype of human breast cancer. The present study aimed to elucidate the potential tumor-suppressive function of MATR3, an abundant nuclear protein, in BLBC/TNBC, whos...

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Autores principales:	Yang,Jaehyuk, Lee,Seung Jun, Kwon,Yongseok, Ma,Li, Kim,Jongchan
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	MATR3 Basal-like breast cancer Triple-negative breast cancer Apoptosis Tumor suppressor Epithelial-mesenchymal transition YAP/TAZ Biomarker
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100232
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spelling	oai:scielo:S0716-976020200001002322020-10-20Tumor suppressive function of Matrin 3 in the basal-like breast cancerYang,JaehyukLee,Seung JunKwon,YongseokMa,LiKim,Jongchan MATR3 Basal-like breast cancer Triple-negative breast cancer Apoptosis Tumor suppressor Epithelial-mesenchymal transition YAP/TAZ Biomarker Abstract Background: Basal-like breast cancer (BLBC) or triple-negative breast cancer (TNBC) is an aggressive and highly metastatic subtype of human breast cancer. The present study aimed to elucidate the potential tumor-suppressive function of MATR3, an abundant nuclear protein, in BLBC/TNBC, whose cancer-relevance has not been characterized. Methods: We analyzed in vitro tumorigenecity by cell proliferation and soft agar colony formation assays, apoptotic cell death by flow cytometry and Poly (ADP-ribose) polymerase (PARP) cleavage, epithelial-mesenchymal transition (EMT) by checking specific EMT markers with real-time quantitative PCR and in vitro migration and invasion by Boyden Chamber assays. To elucidate the underlying mechanism by which MATR3 functions as a tumor suppressor, we performed Tandem affinity purification followed by mass spectrometry (TAP-MS) and pathway analysis. We also scrutinized MATR3 expression levels in the different subtypes of human breast cancer and the correlation between MATR3 expression and patient survival by bioinformatic analyses of publicly available transcriptome datasets. Results: MATR3 suppressed in vitro tumorigenecity, promoted apoptotic cell death and inhibited EMT, migration, and invasion in BLBC/TNBC cells. Various proteins regulating apoptosis were identified as MATR3-binding proteins, and YAP/TAZ pathway was suppressed by MATR3. MATR3 expression was inversely correlated with the aggressive and metastatic nature of breast cancer. Moreover, high expression levels of MATR3 were associated with a good prognosis of breast cancer patients. Conclusions: Our data demonstrate that MATR3 functions as a putative tumor suppressor in BLBC/TNBC cells. Also, MATR3 potentially plays a role as a biomarker in predicting chemotherapy-sensitivity and patient survival in breast cancer patients.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100232en10.1186/s40659-020-00310-6
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	MATR3 Basal-like breast cancer Triple-negative breast cancer Apoptosis Tumor suppressor Epithelial-mesenchymal transition YAP/TAZ Biomarker
spellingShingle	MATR3 Basal-like breast cancer Triple-negative breast cancer Apoptosis Tumor suppressor Epithelial-mesenchymal transition YAP/TAZ Biomarker Yang,Jaehyuk Lee,Seung Jun Kwon,Yongseok Ma,Li Kim,Jongchan Tumor suppressive function of Matrin 3 in the basal-like breast cancer
description	Abstract Background: Basal-like breast cancer (BLBC) or triple-negative breast cancer (TNBC) is an aggressive and highly metastatic subtype of human breast cancer. The present study aimed to elucidate the potential tumor-suppressive function of MATR3, an abundant nuclear protein, in BLBC/TNBC, whose cancer-relevance has not been characterized. Methods: We analyzed in vitro tumorigenecity by cell proliferation and soft agar colony formation assays, apoptotic cell death by flow cytometry and Poly (ADP-ribose) polymerase (PARP) cleavage, epithelial-mesenchymal transition (EMT) by checking specific EMT markers with real-time quantitative PCR and in vitro migration and invasion by Boyden Chamber assays. To elucidate the underlying mechanism by which MATR3 functions as a tumor suppressor, we performed Tandem affinity purification followed by mass spectrometry (TAP-MS) and pathway analysis. We also scrutinized MATR3 expression levels in the different subtypes of human breast cancer and the correlation between MATR3 expression and patient survival by bioinformatic analyses of publicly available transcriptome datasets. Results: MATR3 suppressed in vitro tumorigenecity, promoted apoptotic cell death and inhibited EMT, migration, and invasion in BLBC/TNBC cells. Various proteins regulating apoptosis were identified as MATR3-binding proteins, and YAP/TAZ pathway was suppressed by MATR3. MATR3 expression was inversely correlated with the aggressive and metastatic nature of breast cancer. Moreover, high expression levels of MATR3 were associated with a good prognosis of breast cancer patients. Conclusions: Our data demonstrate that MATR3 functions as a putative tumor suppressor in BLBC/TNBC cells. Also, MATR3 potentially plays a role as a biomarker in predicting chemotherapy-sensitivity and patient survival in breast cancer patients.
author	Yang,Jaehyuk Lee,Seung Jun Kwon,Yongseok Ma,Li Kim,Jongchan
author_facet	Yang,Jaehyuk Lee,Seung Jun Kwon,Yongseok Ma,Li Kim,Jongchan
author_sort	Yang,Jaehyuk
title	Tumor suppressive function of Matrin 3 in the basal-like breast cancer
title_short	Tumor suppressive function of Matrin 3 in the basal-like breast cancer
title_full	Tumor suppressive function of Matrin 3 in the basal-like breast cancer
title_fullStr	Tumor suppressive function of Matrin 3 in the basal-like breast cancer
title_full_unstemmed	Tumor suppressive function of Matrin 3 in the basal-like breast cancer
title_sort	tumor suppressive function of matrin 3 in the basal-like breast cancer
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100232
work_keys_str_mv	AT yangjaehyuk tumorsuppressivefunctionofmatrin3inthebasallikebreastcancer AT leeseungjun tumorsuppressivefunctionofmatrin3inthebasallikebreastcancer AT kwonyongseok tumorsuppressivefunctionofmatrin3inthebasallikebreastcancer AT mali tumorsuppressivefunctionofmatrin3inthebasallikebreastcancer AT kimjongchan tumorsuppressivefunctionofmatrin3inthebasallikebreastcancer
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Tumor suppressive function of Matrin 3 in the basal-like breast cancer

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