Short-term perinatal oxygen exposure may impair lung development in adult mice

Abstract Background: Hyperoxia at resuscitation increases oxidative stress, and even brief exposure to high oxygen concentrations during stabilization may trigger organ injury with adverse long-term outcomes in premature infants. We studied the long-term effects of short-term perinatal oxygen expos...

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Autores principales:	Kumar,Vasantha H. S., Wang,Huamei, Nielsen,Lori
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	Oxygen Gene expression Cell cycle Lung Resuscitation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100238
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spelling	oai:scielo:S0716-976020200001002382020-11-24Short-term perinatal oxygen exposure may impair lung development in adult miceKumar,Vasantha H. S.Wang,HuameiNielsen,Lori Oxygen Gene expression Cell cycle Lung Resuscitation Abstract Background: Hyperoxia at resuscitation increases oxidative stress, and even brief exposure to high oxygen concentrations during stabilization may trigger organ injury with adverse long-term outcomes in premature infants. We studied the long-term effects of short-term perinatal oxygen exposure on cell cycle gene expression and lung growth in adult mice. Methods: We randomized mice litters at birth to 21,40, or 100%O2 for 30 min and recovered in room air for 4 or 12 weeks. Cell cycle gene expression, protein analysis, and lung morphometry were assessed at 4 and 12 weeks. Results: The principal component analysis demonstrated a high degree of correlation for cell cycle gene expression among the three oxygen groups. Lung elastin was significantly lower in the 100%O2 groups at 4 weeks. On lung morphometry, radial alveolar count, alveolar number, and septal count were similar. However, the mean linear intercept (MLI) and septal length significantly correlated among the oxygen groups. The MLI was markedly higher in the 100%O2 groups at 4 and 12 weeks of age, and the septal length was significantly lower in the 100%O2 groups at 12 weeks. Conclusion: Short-term exposure to high oxygen concentrations lead to subtle changes in lung development that may affect alveolarization. The changes are related explicitly to secondary crest formation that may result in alteration in lung elastin. Resuscitation with high oxygen concentrations may have a significant impact on lung development and long-term outcomes such as BPD in premature infants.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100238en10.1186/s40659-020-00318-y
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Oxygen Gene expression Cell cycle Lung Resuscitation
spellingShingle	Oxygen Gene expression Cell cycle Lung Resuscitation Kumar,Vasantha H. S. Wang,Huamei Nielsen,Lori Short-term perinatal oxygen exposure may impair lung development in adult mice
description	Abstract Background: Hyperoxia at resuscitation increases oxidative stress, and even brief exposure to high oxygen concentrations during stabilization may trigger organ injury with adverse long-term outcomes in premature infants. We studied the long-term effects of short-term perinatal oxygen exposure on cell cycle gene expression and lung growth in adult mice. Methods: We randomized mice litters at birth to 21,40, or 100%O2 for 30 min and recovered in room air for 4 or 12 weeks. Cell cycle gene expression, protein analysis, and lung morphometry were assessed at 4 and 12 weeks. Results: The principal component analysis demonstrated a high degree of correlation for cell cycle gene expression among the three oxygen groups. Lung elastin was significantly lower in the 100%O2 groups at 4 weeks. On lung morphometry, radial alveolar count, alveolar number, and septal count were similar. However, the mean linear intercept (MLI) and septal length significantly correlated among the oxygen groups. The MLI was markedly higher in the 100%O2 groups at 4 and 12 weeks of age, and the septal length was significantly lower in the 100%O2 groups at 12 weeks. Conclusion: Short-term exposure to high oxygen concentrations lead to subtle changes in lung development that may affect alveolarization. The changes are related explicitly to secondary crest formation that may result in alteration in lung elastin. Resuscitation with high oxygen concentrations may have a significant impact on lung development and long-term outcomes such as BPD in premature infants.
author	Kumar,Vasantha H. S. Wang,Huamei Nielsen,Lori
author_facet	Kumar,Vasantha H. S. Wang,Huamei Nielsen,Lori
author_sort	Kumar,Vasantha H. S.
title	Short-term perinatal oxygen exposure may impair lung development in adult mice
title_short	Short-term perinatal oxygen exposure may impair lung development in adult mice
title_full	Short-term perinatal oxygen exposure may impair lung development in adult mice
title_fullStr	Short-term perinatal oxygen exposure may impair lung development in adult mice
title_full_unstemmed	Short-term perinatal oxygen exposure may impair lung development in adult mice
title_sort	short-term perinatal oxygen exposure may impair lung development in adult mice
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100238
work_keys_str_mv	AT kumarvasanthahs shorttermperinataloxygenexposuremayimpairlungdevelopmentinadultmice AT wanghuamei shorttermperinataloxygenexposuremayimpairlungdevelopmentinadultmice AT nielsenlori shorttermperinataloxygenexposuremayimpairlungdevelopmentinadultmice
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Short-term perinatal oxygen exposure may impair lung development in adult mice

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