Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment

Abstract Objectives: Our previous study indicated that aerobic exercise relieves cognitive impairment in patients with vascular cognitive impairment (VCI) via regulating brain-derived neurotrophic factor (BDNF), but the mechanism is not yet clear. This study aimed to explore whether lncRNA taurine...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang,Jing, Niu,Yali, Tao,Huaying, Xue,Mina, Wan,Chunxiao
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	Vascular cognitive impairment lncRNA TUG1 BDNF Aerobic exercise
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100240
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602020000100240
record_format	dspace
spelling	oai:scielo:S0716-976020200001002402021-01-14Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairmentWang,JingNiu,YaliTao,HuayingXue,MinaWan,Chunxiao Vascular cognitive impairment lncRNA TUG1 BDNF Aerobic exercise Abstract Objectives: Our previous study indicated that aerobic exercise relieves cognitive impairment in patients with vascular cognitive impairment (VCI) via regulating brain-derived neurotrophic factor (BDNF), but the mechanism is not yet clear. This study aimed to explore whether lncRNA taurine upregulated gene 1 (TUG1) participates in the process of VCI by regulating BDNF. Methods: The expressions of TUG1 and BDNF in the serum of VCI patients were detected. The potential molecular mechanisms of TUG1 in regulating hippocampal neuronal apoptosis were explored in oxygen and glucose deprivation-induced (OGD-induced) hippocampal cell line HT22. The VCI mouse model was established, and TUG1 and BDNF were overexpressed via lentivirus injection. The cognitive impairment of mice was detected by the Morris water maze experiment after the aerobic exercise. Results: The level of TUG1 was elevated in the serum of VCI patients compared with the control group. The knockdown of TUG1 in OGD-induced HT22 cells increased BDNF level and decreased cell apoptosis, and the downregulation of BDNF restored the decreased cell apoptosis. RNA immunoprecipitation and RNA pull-down assays showed that TUG1 could bind to BDNF protein. The aerobic exercise alleviated cognitive impairment and inhibited hippocampal apoptosis in VCI mice. Meanwhile, the overexpression of TUG1 reversed the therapeutic effects of aerobic exercise on cognitive impairment. Conclusions: The knockdown of TUG1 reduced hippocampal neuronal apoptosis and participates in the aerobic exercise-alleviated VCI, which was partly through regulating BDNF.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100240en10.1186/s40659-020-00320-4
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Vascular cognitive impairment lncRNA TUG1 BDNF Aerobic exercise
spellingShingle	Vascular cognitive impairment lncRNA TUG1 BDNF Aerobic exercise Wang,Jing Niu,Yali Tao,Huaying Xue,Mina Wan,Chunxiao Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment
description	Abstract Objectives: Our previous study indicated that aerobic exercise relieves cognitive impairment in patients with vascular cognitive impairment (VCI) via regulating brain-derived neurotrophic factor (BDNF), but the mechanism is not yet clear. This study aimed to explore whether lncRNA taurine upregulated gene 1 (TUG1) participates in the process of VCI by regulating BDNF. Methods: The expressions of TUG1 and BDNF in the serum of VCI patients were detected. The potential molecular mechanisms of TUG1 in regulating hippocampal neuronal apoptosis were explored in oxygen and glucose deprivation-induced (OGD-induced) hippocampal cell line HT22. The VCI mouse model was established, and TUG1 and BDNF were overexpressed via lentivirus injection. The cognitive impairment of mice was detected by the Morris water maze experiment after the aerobic exercise. Results: The level of TUG1 was elevated in the serum of VCI patients compared with the control group. The knockdown of TUG1 in OGD-induced HT22 cells increased BDNF level and decreased cell apoptosis, and the downregulation of BDNF restored the decreased cell apoptosis. RNA immunoprecipitation and RNA pull-down assays showed that TUG1 could bind to BDNF protein. The aerobic exercise alleviated cognitive impairment and inhibited hippocampal apoptosis in VCI mice. Meanwhile, the overexpression of TUG1 reversed the therapeutic effects of aerobic exercise on cognitive impairment. Conclusions: The knockdown of TUG1 reduced hippocampal neuronal apoptosis and participates in the aerobic exercise-alleviated VCI, which was partly through regulating BDNF.
author	Wang,Jing Niu,Yali Tao,Huaying Xue,Mina Wan,Chunxiao
author_facet	Wang,Jing Niu,Yali Tao,Huaying Xue,Mina Wan,Chunxiao
author_sort	Wang,Jing
title	Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment
title_short	Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment
title_full	Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment
title_fullStr	Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment
title_full_unstemmed	Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment
title_sort	knockdown of lncrna tug1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100240
work_keys_str_mv	AT wangjing knockdownoflncrnatug1inhibitshippocampalneuronalapoptosisandparticipatesinaerobicexercisealleviatedvascularcognitiveimpairment AT niuyali knockdownoflncrnatug1inhibitshippocampalneuronalapoptosisandparticipatesinaerobicexercisealleviatedvascularcognitiveimpairment AT taohuaying knockdownoflncrnatug1inhibitshippocampalneuronalapoptosisandparticipatesinaerobicexercisealleviatedvascularcognitiveimpairment AT xuemina knockdownoflncrnatug1inhibitshippocampalneuronalapoptosisandparticipatesinaerobicexercisealleviatedvascularcognitiveimpairment AT wanchunxiao knockdownoflncrnatug1inhibitshippocampalneuronalapoptosisandparticipatesinaerobicexercisealleviatedvascularcognitiveimpairment
_version_	1718441610248716288

Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment

Ejemplares similares