Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species

Abstract Background: We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. Results: Using a genomic...

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Autores principales:	Fernandes-Rocha,Lucas, Ribeiro-Paula,Natália, Gonçalves-Nazareno,Alison, Carvalho,Dulcinéia de
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2020
Materias:	Eremanthus erythropappus Simple sequence repeats Genetic diversity Eremanthus incanus Eremanthus glomerulatus
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100401
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spelling	oai:scielo:S0716-976020200001004012020-07-27Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related speciesFernandes-Rocha,LucasRibeiro-Paula,NatáliaGonçalves-Nazareno,AlisonCarvalho,Dulcinéia de Eremanthus erythropappus Simple sequence repeats Genetic diversity Eremanthus incanus Eremanthus glomerulatus Abstract Background: We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. Results: Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed ( HO ) and expected ( HE ) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively. Conclusions: These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.53 20202020-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100401en10.1186/s40659-020-00298-z
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Eremanthus erythropappus Simple sequence repeats Genetic diversity Eremanthus incanus Eremanthus glomerulatus
spellingShingle	Eremanthus erythropappus Simple sequence repeats Genetic diversity Eremanthus incanus Eremanthus glomerulatus Fernandes-Rocha,Lucas Ribeiro-Paula,Natália Gonçalves-Nazareno,Alison Carvalho,Dulcinéia de Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species
description	Abstract Background: We developed simple sequence repeats (SSR) for Eremanthus erythropappus (DC.) MacLeish, an endangered tree species endemic to the Brazilian Savanna and Atlantic Forest biomes, and tested their transferability to two closely related Eremanthus species. Results: Using a genomic library enriched with tandem repeat motifs, we identified 16 primer pairs, and characterized them in two populations. Nine primers amplified the expected size fragments and seven SSRs were polymorphic, providing a total of 38 alleles and an average of 4.22 alleles per marker. The polymorphic information content (PIC) ranged from 0.44 to 0.94 with an average of 0.65. The average observed heterozygosity across all loci varied from 0.61 to 1.00. The observed ( HO ) and expected ( HE ) heterozygosity within the two populations varied from 0.65 to 1.00 and from 0.31 to 1.00, respectively. Conclusions: These newly developed SSR markers are a powerful tool for population genetic analyses and may be useful in studies on species ecology, evolution, and taxonomy.
author	Fernandes-Rocha,Lucas Ribeiro-Paula,Natália Gonçalves-Nazareno,Alison Carvalho,Dulcinéia de
author_facet	Fernandes-Rocha,Lucas Ribeiro-Paula,Natália Gonçalves-Nazareno,Alison Carvalho,Dulcinéia de
author_sort	Fernandes-Rocha,Lucas
title	Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species
title_short	Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species
title_full	Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species
title_fullStr	Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species
title_full_unstemmed	Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species
title_sort	development and characterization of nuclear microsatellite markers for eremanthus erythropappus and their transferability across related species
publisher	Sociedad de Biología de Chile
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602020000100401
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Development and characterization of nuclear microsatellite markers for Eremanthus erythropappus and their transferability across related species

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