Low-Temperature Extracts of Purple Blossoms of Basil (Ocimum Basilicum L.) Intervened Mitochondrial Translocation Contributes Prompted Apoptosis in Human Breast Cancer Cells

Abstract Background: The preventive and therapeutic medical utilization of this plant is an age-long practice across the globe. This study aimed to validate the impact of dark purple blossoms of basil (Ocimum basilicum L.) aqueous extract at low temperature (0 °C) mediated mitochondrial fission con...

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Autores principales:	Alkhateeb,Mariam Abdulaziz, Al-Otaibi,Wedad Refaiea, AlGabbani,Qwait, Alsakran,Amena Ali, Alnafjan,Alaa Ahmed, Alotaibi,Amani Mohammed, Al-Qahtani,Wedad Saeed
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2021
Materias:	Basil (ocimum basilicum l.) Purple blossoms Aqueous extract at low temperature (0 °C/6 h) Mitochondrial fission Apoptosis Human breast cancer cells (MCF7) Anticancer
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100202
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spelling	oai:scielo:S0716-976020210001002022021-03-15Low-Temperature Extracts of Purple Blossoms of Basil (Ocimum Basilicum L.) Intervened Mitochondrial Translocation Contributes Prompted Apoptosis in Human Breast Cancer CellsAlkhateeb,Mariam AbdulazizAl-Otaibi,Wedad RefaieaAlGabbani,QwaitAlsakran,Amena AliAlnafjan,Alaa AhmedAlotaibi,Amani MohammedAl-Qahtani,Wedad Saeed Basil (ocimum basilicum l.) Purple blossoms Aqueous extract at low temperature (0 °C/6 h) Mitochondrial fission Apoptosis Human breast cancer cells (MCF7) Anticancer Abstract Background: The preventive and therapeutic medical utilization of this plant is an age-long practice across the globe. This study aimed to validate the impact of dark purple blossoms of basil (Ocimum basilicum L.) aqueous extract at low temperature (0 °C) mediated mitochondrial fission contributed to induced apoptosis in human breast cancer cells. Methods: Fresh blossoms were extracted at low temperature (0 °C) using a watery solvent. Human MCF7 breast cancer cells were then treated with 3 separate fluctuated concentrations of 0, 50, 150 and 250 μg/mL for 24 and 48 h. Results: The outcomes demonstrated the presence of anthocyanins, anthraquinones, tannins, reducing sugars, glycosides, proteins, amino acids, flavonoids and volatile oils and nonappearance of Terpinoids and alkaloids. Contrastingly, frail presence of steroids in basil blossoms aqueous concentrate was noted. In addition, the results from a phytochemical subjective examination of basil (Ocimum basilicum L.) blossoms aqueous extract demonstrated that most of the credited natural impacts containing more remarkable contents of antioxidants and anticancer compounds in basil blossoms aqueous extract. Moreover, the restraint of glucose take-up was alleviated mediated by a dose-dependent manner in MCF7 cells with basil (Ocimum basilicum L.) blossoms aqueous extract inducted for 24 h, resulting in mitochondrial fission. Conclusion: This is the first study that shows the impact of the aqueous extract of basil (Ocimum basilicum L.) blossoms was extracted at low temperature (0°C/6 h) underlined high amounts of flavonoids and phenolic compounds bearing more anticancer and antioxidant activities compared to another aqueous extract (using boiled water solvent) and alcoholic extracts.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.54 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100202en10.1186/s40659-020-00324-0
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Basil (ocimum basilicum l.) Purple blossoms Aqueous extract at low temperature (0 °C/6 h) Mitochondrial fission Apoptosis Human breast cancer cells (MCF7) Anticancer
spellingShingle	Basil (ocimum basilicum l.) Purple blossoms Aqueous extract at low temperature (0 °C/6 h) Mitochondrial fission Apoptosis Human breast cancer cells (MCF7) Anticancer Alkhateeb,Mariam Abdulaziz Al-Otaibi,Wedad Refaiea AlGabbani,Qwait Alsakran,Amena Ali Alnafjan,Alaa Ahmed Alotaibi,Amani Mohammed Al-Qahtani,Wedad Saeed Low-Temperature Extracts of Purple Blossoms of Basil (Ocimum Basilicum L.) Intervened Mitochondrial Translocation Contributes Prompted Apoptosis in Human Breast Cancer Cells
description	Abstract Background: The preventive and therapeutic medical utilization of this plant is an age-long practice across the globe. This study aimed to validate the impact of dark purple blossoms of basil (Ocimum basilicum L.) aqueous extract at low temperature (0 °C) mediated mitochondrial fission contributed to induced apoptosis in human breast cancer cells. Methods: Fresh blossoms were extracted at low temperature (0 °C) using a watery solvent. Human MCF7 breast cancer cells were then treated with 3 separate fluctuated concentrations of 0, 50, 150 and 250 μg/mL for 24 and 48 h. Results: The outcomes demonstrated the presence of anthocyanins, anthraquinones, tannins, reducing sugars, glycosides, proteins, amino acids, flavonoids and volatile oils and nonappearance of Terpinoids and alkaloids. Contrastingly, frail presence of steroids in basil blossoms aqueous concentrate was noted. In addition, the results from a phytochemical subjective examination of basil (Ocimum basilicum L.) blossoms aqueous extract demonstrated that most of the credited natural impacts containing more remarkable contents of antioxidants and anticancer compounds in basil blossoms aqueous extract. Moreover, the restraint of glucose take-up was alleviated mediated by a dose-dependent manner in MCF7 cells with basil (Ocimum basilicum L.) blossoms aqueous extract inducted for 24 h, resulting in mitochondrial fission. Conclusion: This is the first study that shows the impact of the aqueous extract of basil (Ocimum basilicum L.) blossoms was extracted at low temperature (0°C/6 h) underlined high amounts of flavonoids and phenolic compounds bearing more anticancer and antioxidant activities compared to another aqueous extract (using boiled water solvent) and alcoholic extracts.
author	Alkhateeb,Mariam Abdulaziz Al-Otaibi,Wedad Refaiea AlGabbani,Qwait Alsakran,Amena Ali Alnafjan,Alaa Ahmed Alotaibi,Amani Mohammed Al-Qahtani,Wedad Saeed
author_facet	Alkhateeb,Mariam Abdulaziz Al-Otaibi,Wedad Refaiea AlGabbani,Qwait Alsakran,Amena Ali Alnafjan,Alaa Ahmed Alotaibi,Amani Mohammed Al-Qahtani,Wedad Saeed
author_sort	Alkhateeb,Mariam Abdulaziz
title	Low-Temperature Extracts of Purple Blossoms of Basil (Ocimum Basilicum L.) Intervened Mitochondrial Translocation Contributes Prompted Apoptosis in Human Breast Cancer Cells
title_short	Low-Temperature Extracts of Purple Blossoms of Basil (Ocimum Basilicum L.) Intervened Mitochondrial Translocation Contributes Prompted Apoptosis in Human Breast Cancer Cells
title_full	Low-Temperature Extracts of Purple Blossoms of Basil (Ocimum Basilicum L.) Intervened Mitochondrial Translocation Contributes Prompted Apoptosis in Human Breast Cancer Cells
title_fullStr	Low-Temperature Extracts of Purple Blossoms of Basil (Ocimum Basilicum L.) Intervened Mitochondrial Translocation Contributes Prompted Apoptosis in Human Breast Cancer Cells
title_full_unstemmed	Low-Temperature Extracts of Purple Blossoms of Basil (Ocimum Basilicum L.) Intervened Mitochondrial Translocation Contributes Prompted Apoptosis in Human Breast Cancer Cells
title_sort	low-temperature extracts of purple blossoms of basil (ocimum basilicum l.) intervened mitochondrial translocation contributes prompted apoptosis in human breast cancer cells
publisher	Sociedad de Biología de Chile
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100202
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Low-Temperature Extracts of Purple Blossoms of Basil (Ocimum Basilicum L.) Intervened Mitochondrial Translocation Contributes Prompted Apoptosis in Human Breast Cancer Cells

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