Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice

Abstract Background: Mitochondria play a significant role in plant cytoplasmic male sterility (CMS). In our previous study, mitochondrial complex I genes, nad4, nad5, and nad7 showed polymorphisms between the transgenic CMS line M2BS and its wild type M2B. The sterility mechanism of the M2BS at cyt...

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Autores principales:	Tang,Danfeng, Wei,Fan, Khan,Aziz, Munsif,Fazal, Zhou,Ruiyang
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2021
Materias:	Rice Transgenic CMS line TEM Mitochondrial biochemistry
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100206
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spelling	oai:scielo:S0716-976020210001002062021-03-15Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of riceTang,DanfengWei,FanKhan,AzizMunsif,FazalZhou,Ruiyang Rice Transgenic CMS line TEM Mitochondrial biochemistry Abstract Background: Mitochondria play a significant role in plant cytoplasmic male sterility (CMS). In our previous study, mitochondrial complex I genes, nad4, nad5, and nad7 showed polymorphisms between the transgenic CMS line M2BS and its wild type M2B. The sterility mechanism of the M2BS at cytological, physiological, biochemical, and molecular level is not clear. Results: Cytological observation showed that the anthers were light yellow, fissured, invalid in KI-I2, and full of irregularly typical abortion pollen grains in M2BS. Transmission electron microscopic (TEM) observation revealed no nucleus and degraded mitochondria with obscure cristae in anther cells of M2BS. The results of staining for H2O2 presented a large number of electron dense precipitates (edp) in intercellular space of anther cells of M2BS at anthesis. Moreover, the anther respiration rate and complex I activity of M2BS were significantly lower than those of wild type M2B during pollen development. Furthermore, RNA editing results showed only nad7 presented partially edited at 534th nucleotides. The expression of nad5 and nad7 revealed significant differences between M2B and M2BS. Conclusions: Our data demonstrated that mitochondrial structural degradation and complex I deficiency might be associated with transgenic CMS of rice.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.54 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100206en10.1186/s40659-020-00326-y
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Rice Transgenic CMS line TEM Mitochondrial biochemistry
spellingShingle	Rice Transgenic CMS line TEM Mitochondrial biochemistry Tang,Danfeng Wei,Fan Khan,Aziz Munsif,Fazal Zhou,Ruiyang Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice
description	Abstract Background: Mitochondria play a significant role in plant cytoplasmic male sterility (CMS). In our previous study, mitochondrial complex I genes, nad4, nad5, and nad7 showed polymorphisms between the transgenic CMS line M2BS and its wild type M2B. The sterility mechanism of the M2BS at cytological, physiological, biochemical, and molecular level is not clear. Results: Cytological observation showed that the anthers were light yellow, fissured, invalid in KI-I2, and full of irregularly typical abortion pollen grains in M2BS. Transmission electron microscopic (TEM) observation revealed no nucleus and degraded mitochondria with obscure cristae in anther cells of M2BS. The results of staining for H2O2 presented a large number of electron dense precipitates (edp) in intercellular space of anther cells of M2BS at anthesis. Moreover, the anther respiration rate and complex I activity of M2BS were significantly lower than those of wild type M2B during pollen development. Furthermore, RNA editing results showed only nad7 presented partially edited at 534th nucleotides. The expression of nad5 and nad7 revealed significant differences between M2B and M2BS. Conclusions: Our data demonstrated that mitochondrial structural degradation and complex I deficiency might be associated with transgenic CMS of rice.
author	Tang,Danfeng Wei,Fan Khan,Aziz Munsif,Fazal Zhou,Ruiyang
author_facet	Tang,Danfeng Wei,Fan Khan,Aziz Munsif,Fazal Zhou,Ruiyang
author_sort	Tang,Danfeng
title	Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice
title_short	Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice
title_full	Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice
title_fullStr	Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice
title_full_unstemmed	Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice
title_sort	degradation of mitochondrial structure and deficiency of complex i were associated with the transgenic cms of rice
publisher	Sociedad de Biología de Chile
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100206
work_keys_str_mv	AT tangdanfeng degradationofmitochondrialstructureanddeficiencyofcomplexiwereassociatedwiththetransgeniccmsofrice AT weifan degradationofmitochondrialstructureanddeficiencyofcomplexiwereassociatedwiththetransgeniccmsofrice AT khanaziz degradationofmitochondrialstructureanddeficiencyofcomplexiwereassociatedwiththetransgeniccmsofrice AT munsiffazal degradationofmitochondrialstructureanddeficiencyofcomplexiwereassociatedwiththetransgeniccmsofrice AT zhouruiyang degradationofmitochondrialstructureanddeficiencyofcomplexiwereassociatedwiththetransgeniccmsofrice
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Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice

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