Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway

Abstract Background: Leaves of the natural plant lotus are used in traditional Chinese medicine and tea production. They are rich in flavonoids. Methods: In this study, lotus leaf flavonoids (LLF) were applied to human lung cancer A549 cells and human small cell lung cancer cells H446 in vitro to...

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Autores principales:	Jia,Xiang-Bo, Zhang,Quan, Xu,Lei, Yao,Wen-Jian, Wei,Li
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2021
Materias:	Human lung cancer A549 cells Flavonoid Lotus leaf C57BL/6J mice mRNA ROS/p38 MAPK pathway
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100207
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spelling	oai:scielo:S0716-976020210001002072021-03-29Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathwayJia,Xiang-BoZhang,QuanXu,LeiYao,Wen-JianWei,Li Human lung cancer A549 cells Flavonoid Lotus leaf C57BL/6J mice mRNA ROS/p38 MAPK pathway Abstract Background: Leaves of the natural plant lotus are used in traditional Chinese medicine and tea production. They are rich in flavonoids. Methods: In this study, lotus leaf flavonoids (LLF) were applied to human lung cancer A549 cells and human small cell lung cancer cells H446 in vitro to verify the effect of LLF on apoptosis in these cells through the ROS/p38 MAPK pathway. Results: LLF had no toxic effect on normal cells at concentrations up to 500 μg/mL, but could significantly inhibit the proliferation of A549 cells and H446 cells. Flow cytometry showed that LLF could induce growth in A549 cells. We also found that LLF could increase ROS and MDA levels, and decrease SOD activity in A549 cells. Furthermore, qRT-PCR and western blot analyses showed that LLF could upregulate the expression of p38 MAPK (p-p38 MAPK), caspase-3, caspase-9, cleaved caspase-3, cleaved caspase-9 and Bax and downregulate the expression of Cu/Zn SOD, CAT, Nrf2, NQO1, HO-1, and Bcl-2 in A549 cells. Results of HPLC showed that LLF mainly contain five active substances: kaemp-feritrin, hyperoside, astragalin, phloridzin, and quercetin. The apoptosis-inducing effect of LLF on A549 cells came from these naturally active compounds. Conclusions: We have shown in this study that LLF is a bioactive substance that can induce apoptosis in A549 cells in vitro, and merits further research and development.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.54 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100207en10.1186/s40659-021-00330-w
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Human lung cancer A549 cells Flavonoid Lotus leaf C57BL/6J mice mRNA ROS/p38 MAPK pathway
spellingShingle	Human lung cancer A549 cells Flavonoid Lotus leaf C57BL/6J mice mRNA ROS/p38 MAPK pathway Jia,Xiang-Bo Zhang,Quan Xu,Lei Yao,Wen-Jian Wei,Li Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway
description	Abstract Background: Leaves of the natural plant lotus are used in traditional Chinese medicine and tea production. They are rich in flavonoids. Methods: In this study, lotus leaf flavonoids (LLF) were applied to human lung cancer A549 cells and human small cell lung cancer cells H446 in vitro to verify the effect of LLF on apoptosis in these cells through the ROS/p38 MAPK pathway. Results: LLF had no toxic effect on normal cells at concentrations up to 500 μg/mL, but could significantly inhibit the proliferation of A549 cells and H446 cells. Flow cytometry showed that LLF could induce growth in A549 cells. We also found that LLF could increase ROS and MDA levels, and decrease SOD activity in A549 cells. Furthermore, qRT-PCR and western blot analyses showed that LLF could upregulate the expression of p38 MAPK (p-p38 MAPK), caspase-3, caspase-9, cleaved caspase-3, cleaved caspase-9 and Bax and downregulate the expression of Cu/Zn SOD, CAT, Nrf2, NQO1, HO-1, and Bcl-2 in A549 cells. Results of HPLC showed that LLF mainly contain five active substances: kaemp-feritrin, hyperoside, astragalin, phloridzin, and quercetin. The apoptosis-inducing effect of LLF on A549 cells came from these naturally active compounds. Conclusions: We have shown in this study that LLF is a bioactive substance that can induce apoptosis in A549 cells in vitro, and merits further research and development.
author	Jia,Xiang-Bo Zhang,Quan Xu,Lei Yao,Wen-Jian Wei,Li
author_facet	Jia,Xiang-Bo Zhang,Quan Xu,Lei Yao,Wen-Jian Wei,Li
author_sort	Jia,Xiang-Bo
title	Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway
title_short	Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway
title_full	Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway
title_fullStr	Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway
title_full_unstemmed	Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway
title_sort	lotus leaf flavonoids induce apoptosis of human lung cancer a549 cells through the ros/p38 mapk pathway
publisher	Sociedad de Biología de Chile
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100207
work_keys_str_mv	AT jiaxiangbo lotusleafflavonoidsinduceapoptosisofhumanlungcancera549cellsthroughtherosp38mapkpathway AT zhangquan lotusleafflavonoidsinduceapoptosisofhumanlungcancera549cellsthroughtherosp38mapkpathway AT xulei lotusleafflavonoidsinduceapoptosisofhumanlungcancera549cellsthroughtherosp38mapkpathway AT yaowenjian lotusleafflavonoidsinduceapoptosisofhumanlungcancera549cellsthroughtherosp38mapkpathway AT weili lotusleafflavonoidsinduceapoptosisofhumanlungcancera549cellsthroughtherosp38mapkpathway
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Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway

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