CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis

Abstract Background: Atherosclerosis (AS) is the most common type in cardiovascular disease. Due to its complex pathogenesis, the exact etiology of AS is unclear. circRNA has been shown to play an essential role in most diseases. However, the underlying mechanism of circRNA in AS has been not under...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang,Xiaohao, Lu,Junying, Zhang,Qinghua, Luo,Qiang, Liu,Bin
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2021
Materias:	CircRNA RSF1 MiR-135b-5p HDAC1 Cell progression Inflammation Atherosclerosis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100211
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602021000100211
record_format	dspace
spelling	oai:scielo:S0716-976020210001002112021-04-28CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosisZhang,XiaohaoLu,JunyingZhang,QinghuaLuo,QiangLiu,Bin CircRNA RSF1 MiR-135b-5p HDAC1 Cell progression Inflammation Atherosclerosis Abstract Background: Atherosclerosis (AS) is the most common type in cardiovascular disease. Due to its complex pathogenesis, the exact etiology of AS is unclear. circRNA has been shown to play an essential role in most diseases. However, the underlying mechanism of circRNA in AS has been not understood clearly. Methods: Quantitative Real-Time PCR assay was used to detect the expression of circRSF1, miR-135b-5p and histone deacetylase 1 (HDAC1). Western blot was applied to the measure of protein expression of HDAC1, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax), cleaved-caspase-3, vascular cell adhesion molecule 1 (VCAM1), intercellular cell adhesion molecule-1 (ICAM1) and E-selectin. MTT assay and flow cytometry were used to detect cell proliferation and apoptosis, respectively. Dual luciferase reporter assay and RIP assay was used to determine the relationship among circRSF1, miR-135b-5p and HDAC1. Besides, an ELISA assay was performed to measure the levels of IL-1β, IL-6, TNF-α and IL-8. Results: In this study, ox-LDL inhibited circRSF1 and HDAC1 expression while upregulated miR-135b-5p expression in Human umbilical vein endothelial cells (HUVECs). Importantly, ox-LDL could inhibit HUVECs growth. Moreover, promotion of circRSF1 or inhibition of miR-135b-5p induced cell proliferation while inhibited apoptosis and inflammation of ox-LDL-treated HUVECs, which was reversed by upregulating miR-135b-5p or downregulating HDCA1 in oxLDL-treated HUVECs. More than that, we verified that circRSF1 directly targeted miR-135b-5p and HDAC1 was a target mRNA of miR-135b-5p in HUVECs. Conclusion: CircRSF1 regulated ox-LDL-induced vascular endothelial cell proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in AS, providing new perspectives and methods for the treatment and diagnosis of AS.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.54 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100211en10.1186/s40659-021-00335-5
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	CircRNA RSF1 MiR-135b-5p HDAC1 Cell progression Inflammation Atherosclerosis
spellingShingle	CircRNA RSF1 MiR-135b-5p HDAC1 Cell progression Inflammation Atherosclerosis Zhang,Xiaohao Lu,Junying Zhang,Qinghua Luo,Qiang Liu,Bin CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis
description	Abstract Background: Atherosclerosis (AS) is the most common type in cardiovascular disease. Due to its complex pathogenesis, the exact etiology of AS is unclear. circRNA has been shown to play an essential role in most diseases. However, the underlying mechanism of circRNA in AS has been not understood clearly. Methods: Quantitative Real-Time PCR assay was used to detect the expression of circRSF1, miR-135b-5p and histone deacetylase 1 (HDAC1). Western blot was applied to the measure of protein expression of HDAC1, B-cell lymphoma-2 (Bcl-2), BCL2-associated X (Bax), cleaved-caspase-3, vascular cell adhesion molecule 1 (VCAM1), intercellular cell adhesion molecule-1 (ICAM1) and E-selectin. MTT assay and flow cytometry were used to detect cell proliferation and apoptosis, respectively. Dual luciferase reporter assay and RIP assay was used to determine the relationship among circRSF1, miR-135b-5p and HDAC1. Besides, an ELISA assay was performed to measure the levels of IL-1β, IL-6, TNF-α and IL-8. Results: In this study, ox-LDL inhibited circRSF1 and HDAC1 expression while upregulated miR-135b-5p expression in Human umbilical vein endothelial cells (HUVECs). Importantly, ox-LDL could inhibit HUVECs growth. Moreover, promotion of circRSF1 or inhibition of miR-135b-5p induced cell proliferation while inhibited apoptosis and inflammation of ox-LDL-treated HUVECs, which was reversed by upregulating miR-135b-5p or downregulating HDCA1 in oxLDL-treated HUVECs. More than that, we verified that circRSF1 directly targeted miR-135b-5p and HDAC1 was a target mRNA of miR-135b-5p in HUVECs. Conclusion: CircRSF1 regulated ox-LDL-induced vascular endothelial cell proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in AS, providing new perspectives and methods for the treatment and diagnosis of AS.
author	Zhang,Xiaohao Lu,Junying Zhang,Qinghua Luo,Qiang Liu,Bin
author_facet	Zhang,Xiaohao Lu,Junying Zhang,Qinghua Luo,Qiang Liu,Bin
author_sort	Zhang,Xiaohao
title	CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis
title_short	CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis
title_full	CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis
title_fullStr	CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis
title_full_unstemmed	CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis
title_sort	circrna rsf1 regulated ox-ldl induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating mir-135b-5p/hdac1 axis in atherosclerosis
publisher	Sociedad de Biología de Chile
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100211
work_keys_str_mv	AT zhangxiaohao circrnarsf1regulatedoxldlinducedvascularendothelialcellsproliferationapoptosisandinflammationthroughmodulatingmir135b5phdac1axisinatherosclerosis AT lujunying circrnarsf1regulatedoxldlinducedvascularendothelialcellsproliferationapoptosisandinflammationthroughmodulatingmir135b5phdac1axisinatherosclerosis AT zhangqinghua circrnarsf1regulatedoxldlinducedvascularendothelialcellsproliferationapoptosisandinflammationthroughmodulatingmir135b5phdac1axisinatherosclerosis AT luoqiang circrnarsf1regulatedoxldlinducedvascularendothelialcellsproliferationapoptosisandinflammationthroughmodulatingmir135b5phdac1axisinatherosclerosis AT liubin circrnarsf1regulatedoxldlinducedvascularendothelialcellsproliferationapoptosisandinflammationthroughmodulatingmir135b5phdac1axisinatherosclerosis
_version_	1718441615754788864

CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis

Ejemplares similares