The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts

Abstract Background: Circular RNAs (circRNAs) has emerged as vital regulator involved in various diseases. In this study, we identified and investigated the potential circRNAs involved in gestational diabetes mellitus (GDM). Methods: High-throughput sequencing was used to collect the plasma circRN...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Chen,Haitian, Zhang,Shaofeng, Wu,Yanxin, Li,Zhuyu, Wang,Dongyu, Cai,Shiqin, Wang,Zilian
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2021
Materias:	Circular RNA Gestational diabetes mellitus circ_0008285 Functional analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100214
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602021000100214
record_format	dspace
spelling	oai:scielo:S0716-976020210001002142021-05-14The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblastsChen,HaitianZhang,ShaofengWu,YanxinLi,ZhuyuWang,DongyuCai,ShiqinWang,Zilian Circular RNA Gestational diabetes mellitus circ_0008285 Functional analysis Abstract Background: Circular RNAs (circRNAs) has emerged as vital regulator involved in various diseases. In this study, we identified and investigated the potential circRNAs involved in gestational diabetes mellitus (GDM). Methods: High-throughput sequencing was used to collect the plasma circRNAs expression profiles of GDM patients. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expressions of circ_0008285 and circ_0001173 in the plasma specimens. The Pearson's correlation test was employed to assess the correlation between 2 circRNAs expression and the clinicopathologic data. Two circRNAs expression was verified in high glucose (HG)-induced HTR-8/SVneo cells. MTS, transwell assay was used to evaluate the effects of circ_0008285 expression on HG-induced HTR-8/SVneo cells. The network of circ_0008285 was constructed using cytocape. Results: In GDM patients, the expression of circ_0008285 was significantly upregulated, while that of circ_0001173 was decreased. Circ_0008285 was significantly correlated with the total cholesterol and LDL-C levels. Circ_0001173 was significantly correlated with glycated hemoglobin. HG promoted the proliferation, invasion, and migration in HTR-8/SVneo cells, while the knockdown of circ_0008285 exerted reverse effects. In addition, network construction exhibited that circ_0008285 had 45 miRNA binding sites, which correlated with 444 mRNA. Conclusions: circ_0008285 plays an important role and provides a clue for the usage of therapeutic targets in the development of GDM.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.54 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100214en10.1186/s40659-021-00337-3
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Circular RNA Gestational diabetes mellitus circ_0008285 Functional analysis
spellingShingle	Circular RNA Gestational diabetes mellitus circ_0008285 Functional analysis Chen,Haitian Zhang,Shaofeng Wu,Yanxin Li,Zhuyu Wang,Dongyu Cai,Shiqin Wang,Zilian The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts
description	Abstract Background: Circular RNAs (circRNAs) has emerged as vital regulator involved in various diseases. In this study, we identified and investigated the potential circRNAs involved in gestational diabetes mellitus (GDM). Methods: High-throughput sequencing was used to collect the plasma circRNAs expression profiles of GDM patients. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to measure the expressions of circ_0008285 and circ_0001173 in the plasma specimens. The Pearson's correlation test was employed to assess the correlation between 2 circRNAs expression and the clinicopathologic data. Two circRNAs expression was verified in high glucose (HG)-induced HTR-8/SVneo cells. MTS, transwell assay was used to evaluate the effects of circ_0008285 expression on HG-induced HTR-8/SVneo cells. The network of circ_0008285 was constructed using cytocape. Results: In GDM patients, the expression of circ_0008285 was significantly upregulated, while that of circ_0001173 was decreased. Circ_0008285 was significantly correlated with the total cholesterol and LDL-C levels. Circ_0001173 was significantly correlated with glycated hemoglobin. HG promoted the proliferation, invasion, and migration in HTR-8/SVneo cells, while the knockdown of circ_0008285 exerted reverse effects. In addition, network construction exhibited that circ_0008285 had 45 miRNA binding sites, which correlated with 444 mRNA. Conclusions: circ_0008285 plays an important role and provides a clue for the usage of therapeutic targets in the development of GDM.
author	Chen,Haitian Zhang,Shaofeng Wu,Yanxin Li,Zhuyu Wang,Dongyu Cai,Shiqin Wang,Zilian
author_facet	Chen,Haitian Zhang,Shaofeng Wu,Yanxin Li,Zhuyu Wang,Dongyu Cai,Shiqin Wang,Zilian
author_sort	Chen,Haitian
title	The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts
title_short	The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts
title_full	The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts
title_fullStr	The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts
title_full_unstemmed	The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts
title_sort	role of circular rna circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts
publisher	Sociedad de Biología de Chile
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602021000100214
work_keys_str_mv	AT chenhaitian theroleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT zhangshaofeng theroleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT wuyanxin theroleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT lizhuyu theroleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT wangdongyu theroleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT caishiqin theroleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT wangzilian theroleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT chenhaitian roleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT zhangshaofeng roleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT wuyanxin roleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT lizhuyu roleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT wangdongyu roleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT caishiqin roleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts AT wangzilian roleofcircularrnacirc0008285ingestationaldiabetesmellitusbyregulatingthebiologicalfunctionsoftrophoblasts
_version_	1718441616710041600

The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts

Ejemplares similares